LOCUS       Exported                1063 bp ds-DNA     linear   SYN 23-MAY-2021
DEFINITION  Mammalian intracellular expression of fluorogen activating protein 
            Mars1Cy-eGFP fusion.
ACCESSION   .
VERSION     .
KEYWORDS    pMars1CyeGFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Zhang M, Chakraborty SK, Sampath P, Rojas JJ, Hou W, Saurabh S, 
            Thorne SH, Bruchez MP, Waggoner AS
  TITLE     Fluoromodule-based reporter/probes designed for in vivo fluorescence
            imaging.
  JOURNAL   J Clin Invest. 2015 Oct 1;125(10):3915-27. doi: 10.1172/JCI81086. 
            Epub 2015 Sep 8.
  PUBMED    26348895
REFERENCE   2  (bases 1 to 1063)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 23, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     polyA_signal    158..279
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(195..214)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     249..268
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(286..741)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(423..444)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     635..654
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        768..872
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(897..917)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 ggcatggacg agctgtacaa gtaacccggg atccaccgga tctagataac tgatcataat
       61 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
      121 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
      181 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
      241 ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa cgcgtaaatt gtaagcgtta
      301 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
      361 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
      421 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
      481 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
      541 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
      601 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
      661 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
      721 atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
      781 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
      841 aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa
      901 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
      961 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     1021 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtc
//
