LOCUS Exported 11748 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Lentiviral transfer vector encoding Mars1Cy-tagged AVPR2. ACCESSION . VERSION . KEYWORDS pLenCMV-PMMars1CyAVPR2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11748) AUTHORS Zhang M, Chakraborty SK, Sampath P, Rojas JJ, Hou W, Saurabh S, Thorne SH, Bruchez MP, Waggoner AS TITLE Fluoromodule-based reporter/probes designed for in vivo fluorescence imaging. JOURNAL J Clin Invest. 2015 Oct 1;125(10):3915-27. doi: 10.1172/JCI81086. Epub 2015 Sep 8. PUBMED 26348895 REFERENCE 2 (bases 1 to 11748) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11748 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(47..66) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 238..617 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 619..817 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" LTR 835..1015 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 1062..1187 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1680..1913 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 2098..2142 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2440..2557 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" enhancer 2662..2965 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 2966..3169 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" regulatory 3208..3217 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" sig_peptide 3214..3276 /product="leader sequence from mouse immunoglobulin kappa light chain" /label=Ig-kappa leader CDS 3277..3303 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" misc_feature 5319..5780 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(5375..5392) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 5602..5621 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 5813..6412 /codon_start=1 /gene="pac from Streptomyces" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA" primer_bind complement(5813..5832) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 6309..6329 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 6440..7028 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(6493..6513) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(6911..6922) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(7031..7047) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7032..7048) /label=pBluescriptKS /note="For pBluescript vector" LTR 7553..7733 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(7759..7776) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 7765..7989 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 8035..8463 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(8122..8141) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 8332..8353 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(8472..8492) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 8477..8806 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 8657..8792 /label=SV40 ori /note="SV40 origin of replication" primer_bind 8719..8738 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 8854..8901 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 8920..9294 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 9424..9545 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(9461..9480) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 9515..9534 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(9594..9610) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(9594..9610) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(9607..9629) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 9618..9634 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(9642..9672) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9687..9708 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(9825..9842) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(9996..10584) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(10076..10095) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(10755..11615) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 11378..11397 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(11616..11720) /gene="bla" /label=AmpR promoter ORIGIN 1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 2581 agatccagtt tggttaatta atagttatta atagtaatca attacggggt cattagttca 2641 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc 2701 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat 2761 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt 2821 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc 2881 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta 2941 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg 3001 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt 3061 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac 3121 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa 3181 ccgtcagatc cgctagcgct accggtcgcc accatggaga cagacacact cctgctatgg 3241 gtactgctgc tctgggttcc aggttccact ggtgactatc catatgatgt tccagattat 3301 gcttctagct cttacgaatt aacacaacct ccatctgttt ctgtttctcc aggtcaaact 3361 gctagaatta cttgttctgg tgatgctttg ccaaaacaat acacttactg gtatcaacaa 3421 aaagctggtc aagctccagt tttggttatt tataaagata ctgaaagacc atctggtatt 3481 ccagaaagat tctcaggtac ttcttctggt actactgcta ctttgactat ttctggtgtt 3541 caagctgaag atgaagctga ttattattgt caatctgctg attcttctgg ttctcatgta 3601 tttttcggtg gtggtactaa agttactgtt ttgtctggtg gaggaggccc aggtggcggt 3661 tcaggtggag gttctggcgg aggttctggt ggaggttcct atgagttgac tcagccaccc 3721 tcggtgtcag tgtccccagg acagacggcc aggatcacct gctctggaga tgcattgcca 3781 aagcaatata cttattggta ccagcagaag gcaggccagg cccctgtctt ggtgatatat 3841 aaagacactg agaggccctc agggatccct gagcgattct ctggtaccag ttcagggaca 3901 acagccacat tgaccatcag tggagtccag gcagaagacg aggctgacta ttactgtcaa 3961 tcagcagaca gcagtggttc ccacgttttc ttcggcggag ggaccaaggt gaccgtacta 4021 tccggcaccg ccgcagctgg cgctggagca gcaggtggag cagctgcagg aatgctcatg 4081 gcgtccacca cttccgctgt gcctgggcat ccctctctgc ccagcctgcc cagcaacagc 4141 agccaggaga ggccactgga cacccgggac ccgctgctag cccgggcgga gctggcgctg 4201 ctctccatag tctttgtggc tgtggccctg agcaatggcc tggtgctggc ggccctagct 4261 cggcggggcc ggcggggcca ctgggcaccc atacacgtct tcattggcca cttgtgcctg 4321 gccgacctgg ccgtggctct gttccaagtg ctgccccagc tggcctggaa ggccaccgac 4381 cgcttccgtg ggccagatgc cctgtgtcgg gccgtgaagt atctgcagat ggtgggcatg 4441 tatgcctcct cctacatgat cctggccatg acgctggacc gccaccgtgc catctgccgt 4501 cccatgctgg cgtaccgcca tggaagtggg gctcactgga accggccggt gctagtggct 4561 tgggccttct cgctccttct cagcctgccc cagctcttca tcttcgccca gcgcaacgtg 4621 gaaggtggca gcggggtcac tgactgctgg gcctgctttg cggagccctg gggccgtcgc 4681 acctatgtca cctggattgc cctgatggtg ttcgtggcac ctaccctggg tatcgccgcc 4741 tgccaggtgc tcatcttccg ggagattcat gccagtctgg tgccagggcc atcagagagg 4801 cctggggggc gccgcagggg acgccggaca ggcagccccg gtgagggagc ccacgtgtca 4861 gcagctgtgg ccaagactgt gaggatgacg ctagtgattg tggtcgtcta tgtgctgtgc 4921 tgggcaccct tcttcctggt gcagctgtgg gccgcgtggg acccggaggc acctctggaa 4981 ggggcgccct ttgtgctact catgttgctg gccagcctca acagctgcac caacccctgg 5041 atctatgcat ctttcagcag cagcgtgtcc tcagagctgc gaagcttgct ctgctgtgcc 5101 cggggacgca ccccacccag cctgggtccc caagatgagt cctgcaccac cgccagctcc 5161 tccctggcca aggacacttc atcgtgatgt acatagtaat gaacggatcc gtaattccgc 5221 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat 5281 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct 5341 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg 5401 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta 5461 gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag 5521 ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg 5581 atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat 5641 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca 5701 tgtgtttagt cgaggttaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc 5761 tttgaaaaac acgatgataa gcttgccaca acccacaagg agacgacctt ccatgaccga 5821 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg tacgcaccct 5881 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgacccgg accgccacat 5941 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa 6001 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga 6061 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct 6121 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg 6181 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt 6241 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc 6301 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga 6361 gtgcccgaag gaccgcgcga cctggtgcat gacccgcaag cccggtgcct gataggaatt 6421 cgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg 6481 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta 6541 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct 6601 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt 6661 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac 6721 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg 6781 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc 6841 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg 6901 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct 6961 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc 7021 ctccccgcat cgataccgtc gacctcgaga cctagaaaaa catggagcaa tcacaagtag 7081 caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt 7141 gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga 7201 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag 7261 acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg attggcagaa 7321 ctacacacca gggccaggga tcagatatcc actgaccttt ggatggtgct acaagctagt 7381 accagttgag caagagaagg tagaagaagc caatgaagga gagaacaccc gcttgttaca 7441 ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtattagagt ggaggtttga 7501 cagccgccta gcatttcatc acatggcccg agagctgcat ccggactgta ctgggtctct 7561 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 7621 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 7681 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagggcccg 7741 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc 7801 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa 7861 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg 7921 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg 7981 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc 8041 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac 8101 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg 8161 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt 8221 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc 8281 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct 8341 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga 8401 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga 8461 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg 8521 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg 8581 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc 8641 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca 8701 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt 8761 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc 8821 ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc ggcatagtat 8881 atcggcatag tataatacga caaggtgagg aactaaacca tggccaagtt gaccagtgcc 8941 gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac cgaccggctc 9001 gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga cgacgtgacc 9061 ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg 9121 gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac gaacttccgg 9181 gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc 9241 ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg 9301 ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc 9361 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac 9421 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 9481 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 9541 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag 9601 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc 9661 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc 9721 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa 9781 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg 9841 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 9901 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 9961 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 10021 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 10081 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 10141 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 10201 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 10261 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 10321 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 10381 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca 10441 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 10501 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 10561 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 10621 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 10681 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 10741 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta 10801 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc 10861 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat 10921 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta 10981 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt 11041 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt 11101 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg 11161 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc 11221 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc 11281 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg 11341 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga 11401 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta 11461 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct 11521 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag 11581 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga 11641 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat 11701 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgac //