LOCUS Exported 14952 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION recipient plasmid [single nuclease]; p35S:Cas9, BASTA. ACCESSION . VERSION . KEYWORDS pDGE63 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 14952) AUTHORS Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, Boch J, Stuttmann J TITLE Generation of chromosomal deletions in dicotyledonous plants employing a user-friendly genome editing toolkit. JOURNAL Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319. PUBMED 27579989 REFERENCE 2 (bases 1 to 14952) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..14952 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 266..290 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA" terminator 333..585 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" CDS complement(605..1156) /codon_start=1 /gene="Streptomyces hygroscopicus bar" /product="phosphinothricin acetyltransferase" /label=BlpR /note="confers resistance to bialophos or phosphinothricin" /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF WQLDFSLPVPPRPVLPVTEI" promoter complement(1199..1378) /label=NOS promoter /note="nopaline synthase promoter" terminator 1471..1723 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" CDS complement(1739..1759) /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS complement(1772..5875) /codon_start=1 /product="Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=Cas9 /note="generates RNA-guided double strand breaks in DNA" /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" promoter complement(5966..6311) /label=CaMV 35S promoter /note="strong constitutive promoter from cauliflower mosaic virus" primer_bind complement(6003..6023) /label=35S promoter /note="CaMV 35S promoter, forward primer" promoter 6842..6944 /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" CDS 6945..7604 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind complement(7011..7030) /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" CDS 7950..8255 /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /label=ccdB /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" primer_bind 8134..8153 /label=ccdB-fwd /note="ccdB gene, forward primer" misc_RNA 8293..8368 /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" misc_feature 8682..8706 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" CDS 10007..10636 /codon_start=1 /product="stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 StaA /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI" CDS 11070..12137 /codon_start=1 /product="replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 RepA /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV MRYRNLIEGEASAGS" rep_origin 12203..12397 /label=pVS1 oriV /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" primer_bind complement(12633..12655) /label=pGEX 3' /note="pGEX vectors, reverse primer" misc_feature 12741..12881 /label=bom /note="basis of mobility region from pBR322" primer_bind 12796..12815 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind complement(12896..12913) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(13067..13655) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(13147..13166) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(13899..14690) /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" ORIGIN 1 ttatgtccac tgggttcgtg cccgaattga tcacaggcag caacgctctg tcatcgttac 61 aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa acccggcagc ttagttgccg 121 ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg gctctcccgc 181 tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc 241 ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac aaattgacgc 301 ttagacaact taataacaca ttgcggaatt ccgatctagt aacatagatg acaccgcgcg 361 cgataattta tcctagtttg cgcgctatat tttgttttct atcgcgtatt aaatgtataa 421 ttgcgggact ctaatcataa aaacccatct cataaataac gtcatgcatt acatgttaat 481 tattacatgc ttaacgtaat tcaacagaaa ttatatgata atcatcgcaa gaccggcaac 541 aggattcaat cttaagaaac tttattgcca aatgtttgaa cgatctgctt gactctaggg 601 gtcatcagat ttcggtgacg ggcaggaccg gacggggcgg caccggcagg ctgaagtcca 661 gctgccagaa acccacgtca tgccagttcc cgtgcttgaa gccggccgcc cgcagcatgc 721 cgcggggggc atatccgagc gcctcgtgca tgcgcacgct cgggtcgttg ggcagcccga 781 tgacagcgac cacgctcttg aagccctgtg cctccaggga cttcagcagg tgggtgtaga 841 gcgtggagcc cagtcccgtc cgctggtggc ggggggagac gtacacggtc gactcggccg 901 tccagtcgta ggcgttgcgt gccttccagg ggcccgcgta ggcgatgccg gcgacctcgc 961 cgtccacctc ggcgacgagc cagggatagc gctcccgcag acggacgagg tcgtccgtcc 1021 actcctgcgg ttcctgcggc tcggtacgga agttgaccgt gcttgtctcg atgtagtggt 1081 tgacgatggt gcagaccgcc ggcatgtccg cctcggtggc acggcggatg tcggccgggc 1141 gtcgttctgg gctcatggta gatccccctc gatcgagttg agagtgaata tgagactcta 1201 attggatacc gaggggaatt tatggaacgt cagtggagca tttttgacaa gaaatatttg 1261 ctagctgata gtgaccttag gcgacttttg aacgcgcaat aatggtttct gacgtatgtg 1321 cttagctcat taaactccag aaacccgcgg ctgagtggct ccttcaacgt tgcggttctg 1381 tcagttccaa acgtaaaacg gcttgtcccg cgtcatcggc gggggtcata acgtgactcc 1441 cttaattctc cgctcatgat tagtagcgtc gatctagtaa catagatgac accgcgcgcg 1501 ataatttatc ctagtttgcg cgctatattt tgttttctat cgcgtattaa atgtataatt 1561 gcgggactct aatcataaaa acccatctca taaataacgt catgcattac atgttaatta 1621 ttacatgctt aacgtaattc aacagaaatt atatgataat catcgcaaga ccggcaacag 1681 gattcaatct taagaaactt tattgccaaa tgtttgaacg atctgcttga caagctcaca 1741 ccttcctctt cttcttgggg tcagccctgc tgtctccacc gagctgagag aggtcgattc 1801 ttgtttcata gagccccgta attgactgat gaatcagtgt ggcgtccagg acctcctttg 1861 tagaggtgta ccgctttctg tctatggtgg tgtcgaagta cttgaaggct gcaggcgcgc 1921 ccaagttggt cagagtaaac aagtggataa tgttttctgc ctgctccctg atgggcttat 1981 ccctgtgctt attgtaagca gaaagcacct tatcgaggtt agcgtcggcg aggatcactc 2041 ttttggagaa ttcgcttatt tgctcgatga tctcatcaag gtagtgtttg tgttgttcca 2101 cgaacagctg cttctgctca ttatcttcgg gagatccttt gagcttttca tagtggctgg 2161 ccagatacaa gaaattaacg tatttagagg gcagtgccag ctcgttacct ttctgcagct 2221 cgcccgcact agcgagcatt cgtttccggc cgttttcaag ctcaaagaga gagtacttgg 2281 gaagcttaat gatgaggtct tttttgacct ctttatatcc tttcgcctcg agaaagtcga 2341 tggggttttt ttcgaagctt gatcgctcca tgattgtgat gcccagcagt tccttgacgc 2401 ttttgagttt tttagacttc cctttctcca ctttggccac aaccagtaca ctgtaagcga 2461 ctgtaggaga atcgaatccg ccgtatttct tggggtccca atcttttttg cgtgcgatca 2521 gcttgtcgct gttccttttc gggaggatac tttccttgga gaagcctccg gtctgtactt 2581 cggtcttttt aacgatgttc acctgcggca tggacaggac cttccggact gtcgcgaaat 2641 ccctaccctt gtcccacacg atttctcctg tttctccgtt tgtttcgata agtggtcgct 2701 tccgaatctc tccattggcc agtgtaatct cggtcttgaa aaaattcata atattgctgt 2761 aaaagaagta cttagcggtg gccttgccta tttcctgctc agactttgcg atcattttcc 2821 taacatcgta cactttatag tctccgtaaa caaattcaga ttcaagcttg ggatattttt 2881 tgataagtgc agtgcctacc actgcattca ggtaggcatc atgcgcatgg tggtaattgt 2941 tgatctctct caccttataa aactgaaagt cctttctgaa atctgaaacc agcttagact 3001 tcagagtaat aactttcacc tctcgaatca gtttgtcatt ttcatcgtac ttggtgttca 3061 tgcgtgaatc gagaatttgg gccacgtgct tggtgatctg gcgtgtctca acaagctgcc 3121 ttttgatgaa gccggcttta tccaactcag acaggccacc tcgttcagcc ttagtcagat 3181 tatcgaactt ccgttgtgtg atcagtttgg cgttcagcag ctgccgccaa taatttttca 3241 ttttcttgac aacttcttct gaggggacgt tatcactctt ccctctattt ttatcggatc 3301 ttgtcaacac tttattatca atagaatcat ctttgagaaa agactggggc acgatatgat 3361 ccacgtcgta gtcggagagc cgattgatgt ccagttcctg atccacgtac atgtccctgc 3421 cgttctgcag gtagtacagg tagagcttct cattctgaag ctgggtgttt tcaactgggt 3481 gttccttaag gatttgggac cccagttctt ttataccctc ttcaatcctc ttcatccttt 3541 ccctactgtt cttctgtccc ttctgggtag tttggttctc tcgggccatc tcgataacga 3601 tattctcggg cttatgcctt cccattactt tgacgagttc atccacgacc ttaacggtct 3661 gcagtattcc ctttttgata gctgggctac ctgcaagatt agcgatgtgc tcgtggagac 3721 tgtccccctg gccagaaact tgtgctttct ggatgtcctc cttaaaggtg agagagtcat 3781 catggatcaa ctgcatgaag ttccggttgg caaatccatc ggacttaaga aaatccagga 3841 ttgtctttcc actctgcttg tctcggatcc cattgatcag ttttcttgac agccgccccc 3901 atcctgtata tcggcgcctc ttgagctgtt tcatgacttt gtcgtcgaag agatgagcgt 3961 aagttttcaa gcgttcttca atcatctccc tatcttcaaa caacgtaagg gtgaggacaa 4021 tgtcctcaag aatgtcctcg ttctcctcat tgtccaggaa gtccttgtct ttaatgattt 4081 tcaggagatc gtgatacgtt cccagggatg cgttgaagcg atcctccact ccgctgattt 4141 caacagagtc gaaacattca atctttttga aataatcttc tttgagctgt ttcacggtaa 4201 ctttccggtt cgtcttgaag aggaggtcca cgatagcttt cttctgctct ccagacagga 4261 atgctggctt tctcatccct tctgtgacgt atttgacctt ggtgagctcg ttataaactg 4321 tgaagtactc gtacagcaga gagtgtttag gaagcacctt ttcgttaggc agatttttat 4381 caaagttagt catcctttcg atgaaggact gggcagaggc ccccttatcc acgacttcct 4441 cgaagttcca gggagtgata gtctcttctg atttgcgagt catccacgcg aatctggaat 4501 ttccccgggc gagggggcct acatagtagg gtatccgaaa tgtgaggatt ttctcaatct 4561 tttccctgtt atctttcaaa aaggggtaga aatcctcttg ccgcctgagg atagcgtgca 4621 gttcgcccag gtgaatctgg tgggggatgc ttccattgtc gaaagtgcgc tgtttgcgca 4681 acagatcttc tctgttaagc tttaccagca gctcctcggt gccgtccatt ttttccaaga 4741 tgggcttaat aaatttgtaa aattcctcct ggcttgctcc gccgtcaatg tatccggcgt 4801 agccattttt agactgatcg aagaaaattt ccttgtactt ctcaggcagt tgctgtctga 4861 caagggcctt cagcaaagtc aagtcttggt ggtgctcatc atagcgcttg atcatactag 4921 cgctcagcgg agctttggtg atctccgtgt tcactcgcag aatatcactc agcagaatgg 4981 cgtctgacag gttctttgcc gccaaaaaaa ggtctgcgta ctggtcgccg atctgggcca 5041 gcagattgtc gagatcatca tcgtaggtgt ctttgctcag ttgaagcttg gcatcttcgg 5101 ccaggtcgaa gttagattta aagttggggg tcagcccgag tgacagggcg ataagattac 5161 caaacaggcc gttcttcttc tccccaggga gctgtgcgat gaggttttcg agccgccggg 5221 atttggacag cctagcgctc aggattgctt tggcgtcaac tccggatgcg ttgatcgggt 5281 tctcttcgaa aagctgattg taagtctgaa ccagttggat aaagagtttg tcgacatcgc 5341 tgttgtctgg gttcaggtcc ccctcgatga ggaagtgtcc ccgaaatttg atcatatgcg 5401 ccagcgcgag atagatcaac cgcaagtcag ccttatcagt actgtctaca agcttcttcc 5461 tcagatgata tatggttggg tacttttcat ggtacgccac ctcgtccacg atattgccaa 5521 agattgggtg gcgctcgtgc tttttatcct cctccaccaa aaaggactcc tccagcctat 5581 ggaagaaaga gtcatccacc ttagccatct cattactaaa gatctcctgc aggtagcaga 5641 tccgattctt tctgcgggta tatctgcgcc gtgctgttct tttgagccgc gtggcttcgg 5701 ccgtctcccc ggagtcgaac aggagggcgc caatgaggtt cttctttatg ctgtggcgat 5761 cggtattgcc cagaactttg aattttttgc tcggcacctt gtactcgtcc gtaatgacgg 5821 cccagccgac gctgtttgtg ccgatatcga gcccaatgga gtacttcttg tccattgtat 5881 cgataattgt aaatgtaatt gtaatgttgt ttgttgtttg ttgttgttgg taattgttgt 5941 aaaaatgagc tcttatactc gagcgtgtcc tctccaaatg aaatgaactt ccttatatag 6001 aggaagggtc ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatgt 6061 cacatcaatc cacttgcttt gtagacgtgg ttggaacctc ttctttttcc acgatgctcc 6121 tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga gagatcttga atgatagcct 6181 ttcctttatc gcaatgatgg catttgtagg agccaccttc cttttctact gtcctttcga 6241 tgaagtgaca gatagctggg caatggaatc cgaggaggtt tcccgaaatt atcctttgtt 6301 gaaaagtctc aatagccctt tgatcttctg agactgtatc tttgacattt ttggagtaga 6361 ccagagtgtc gtgctccacc atgttatcac atcaatccac ttgctttgta gacgtggttg 6421 gaacctcttc tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt 6481 cggcagagag atcttgaatg atagcctttc ctttatcgca atgatggcat ttgtaggagc 6541 caccttcctt ttctactgtc ctttcgatga agtgacagat agctgggcaa tggaatccga 6601 ggaggtttcc cgaaattatc ctttgttgaa aagtctcaat agccctttga tcttctgaga 6661 ctgtatcttt gacatttttg gagtagacca gagtgtcgtg ctccaccatg ttgacctcca 6721 agcttgaatt cttgctaagt tgaaaacaat cttcaaaagt cccacatcgc ttagataaga 6781 aaacgaagct gagtttatat acagctagag tcgaagtagt gattgaagtc ttctgagacg 6841 ttgatcggca cgtaagaggt tccaactttc accataatga aataagatca ctaccgggcg 6901 tattttttga gttatcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca 6961 ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc 7021 agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa 7081 agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc 7141 tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg 7201 atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct 7261 ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt 7321 gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct 7381 cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact 7441 tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc 7501 cgctggcgat tcaggttcat catgccgtct gtgatggctt ccatgtcggc agaatgctta 7561 atgaattaca acagtactgc gatgagtggc aggggggggc gtaaacgctc gcgtggatcc 7621 ggcttactaa aagccagata acagtatgcg tatttgcgcg ctgatttttg cggtataaga 7681 atatatactg atatgtatac ccgaagtatg tcaaaaagag gtatgctatg aagcagcgta 7741 ttacagtgac agttgacagc gacagctatc agttgctcaa ggcatatatg atgtcaatat 7801 ctccggtctg gtaagcacaa ccatgcagaa tgaagcccgt cgtctgcgtg ccgaacgctg 7861 gaaagcggaa aatcaggaag ggatggctga ggtcgcccgg tttattgaaa tgaacggctc 7921 ttttgctgac gagaacaggg gctggtgaaa tgcagtttaa ggtttacacc tataaaagag 7981 agagccgtta tcgtctgttt gtggatgtac agagtgatat tattgacacg cccgggcgac 8041 ggatggtgat ccccctggcc agtgcacgtc tgctgtcaga taaagtctcc cgtgaacttt 8101 acccggtggt gcatatcggg gatgaaagct ggcgcatgat gaccaccgat atggccagtg 8161 tgccggtttc cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca 8221 aaaacgccat taacctgatg ttctggggaa tataaatgtc aggctccctt atacacagcc 8281 agtcgaagac ttgttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca 8341 acttgaaaaa gtggcaccga gtcggtgctt ttttttgctt ccatgctaga gtccgcaaaa 8401 atcaccagtc tctctctaca aatctatctc tctctatttt tctccagaat aatgtgtgag 8461 tagttcccag ataagggaat tagggttctt atagggtttc gctcatgtgt tgagcatata 8521 agaaaccctt agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc 8581 ctaaaaccaa aatccagtga cctgcaggca tgcgacgtcg agcttagctt gagcttggat 8641 cagattgtcg tttcccgcct tcagtttaaa ctatcagtgt ttgacaggat atattggcgg 8701 gtaaacctaa gagaaaagag cgtttattag aataatcgga tatttaaaag ggcgtgaaaa 8761 ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac agggttcccc tcgggatcaa 8821 agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag 8881 ccgtgttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg 8941 cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac 9001 tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc 9061 cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc 9121 tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc 9181 aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg 9241 gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc 9301 ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg 9361 accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc 9421 gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg 9481 gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg 9541 gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa 9601 gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc 9661 gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg 9721 acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg 9781 ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg 9841 gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc 9901 gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg 9961 cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc 10021 tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc 10081 cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg 10141 cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac 10201 gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc 10261 ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt 10321 gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg 10381 cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg 10441 cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga 10501 gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct 10561 tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa 10621 atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta 10681 agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac 10741 acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag 10801 atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag 10861 ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg 10921 cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg 10981 aacgggcggt tggccaggcg taagcggctg ggttgtctgc cggccctgca atggcactgg 11041 aacccccaag cccgaggaat cggcgtgacg gtcgcaaacc atccggcccg gtacaaatcg 11101 gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc 11161 aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc 11221 gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg 11281 gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc 11341 gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg 11401 tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg 11461 ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga 11521 accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg 11581 acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa 11641 cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg 11701 gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga 11761 gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga 11821 tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc 11881 ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca 11941 gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct 12001 gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg 12061 aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag 12121 catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa 12181 aaggtcgaaa aggtgtcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca 12241 ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca 12301 tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac 12361 ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg 12421 aagagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc 12481 gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac 12541 cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc 12601 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg 12661 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg 12721 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat 12781 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg 12841 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc 12901 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 12961 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 13021 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 13081 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 13141 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 13201 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 13261 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 13321 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 13381 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 13441 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 13501 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 13561 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 13621 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 13681 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg catgatatat 13741 ctcccaattt gtgtagggct tattatgcac gcttaaaaat aataaaagca gacttgacct 13801 gatagtttgg ctgtgagcaa ttatgtgctt agtgcatcta atcgcttgag ttaacgccgg 13861 cgaagcggcg tcggcttgaa cgaatttcta gctagacatt atttgccgac taccttggtg 13921 atctcgcctt tcacgtagtg gacaaattct tccaactgat ctgcgcgcga ggccaagcga 13981 tcttcttctt gtccaagata agcctgtcta gcttcaagta tgacgggctg atactgggcc 14041 ggcaggcgct ccattgccca gtcggcagcg acatccttcg gcgcgatttt gccggttact 14101 gcgctgtacc aaatgcggga caacgtaagc actacatttc gctcatcgcc agcccagtcg 14161 ggcggcgagt tccatagcgt taaggtttca tttagcgcct caaatagatc ctgttcagga 14221 accggatcaa agagttcctc cgccgctgga cctaccaagg caacgctatg ttctcttgct 14281 tttgtcagca agatagccag atcaatgtcg atcgtggctg gctcgaagat acctgcaaga 14341 atgtcattgc gctgccattc tccaaattgc agttcgcgct tagctggata acgccacgga 14401 atgatgtcgt cgtgcacaac aatggtgact tctacagcgc ggagaatctc gctctctcca 14461 ggggaagccg aagtttccaa aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc 14521 cttacggtca ccgtaaccag caaatcaata tcactgtgtg gcttcaggcc gccatccact 14581 gcggagccgt acaaatgtac ggccagcaac gtcggttcga gatggcgctc gatgacgcca 14641 actacctctg atagttgagt cgatacttcg gcgatcaccg cttcccccat gatgtttaac 14701 tttgttttag ggcgactgcc ctgctgcgta acatcgttgc tgctccataa catcaaacat 14761 cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg taccccaaaa 14821 aaacatgtca taacaagaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc 14881 ggtcaaggtt ctggaccagt tgcgtgacgg cagttacgct acttgcatta cagcttacga 14941 accgaacgag gc //