LOCUS       Exported               17470 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  recipient plasmid [multiplexing]; p35S:Cas9, FAST, Hygro.
ACCESSION   .
VERSION     .
KEYWORDS    pDGE166
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17470)
  AUTHORS   Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, 
            Boch J, Stuttmann J
  TITLE     Generation of chromosomal deletions in dicotyledonous plants 
            employing a user-friendly genome editing toolkit.
  JOURNAL   Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319.
  PUBMED    27579989
REFERENCE   2  (bases 1 to 17470)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..17470
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             1062..1691
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2125..3192
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      3258..3452
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(3688..3710)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    3796..3936
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     3851..3870
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3951..3968)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4122..4710)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4202..4221)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4954..5745)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    6266..6290
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        6436..6615
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    6651..6706
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             6708..7733
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     terminator      7746..7998
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      10368..10620
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(10636..10656)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(10669..14772)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     promoter        complement(14863..15208)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(14900..14920)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        15647..15749
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             15750..16409
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(15816..15835)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             16751..17056
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     16935..16954
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     misc_feature    17177..17201
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag tagaatactt gcgactagaa
       61 ccggagacat tacgccatga acaagagcgc cgccgctggc ctgctgggct atgcccgcgt
      121 cagcaccgac gaccaggact tgaccaacca acgggccgaa ctgcacgcgg ccggctgcac
      181 caagctgttt tccgagaaga tcaccggcac caggcgcgac cgcccggagc tggccaggat
      241 gcttgaccac ctacgccctg gcgacgttgt gacagtgacc aggctagacc gcctggcccg
      301 cagcacccgc gacctactgg acattgccga gcgcatccag gaggccggcg cgggcctgcg
      361 tagcctggca gagccgtggg ccgacaccac cacgccggcc ggccgcatgg tgttgaccgt
      421 gttcgccggc attgccgagt tcgagcgttc cctaatcatc gaccgcaccc ggagcgggcg
      481 cgaggccgcc aaggcccgag gcgtgaagtt tggcccccgc cctaccctca ccccggcaca
      541 gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc accgtgaaag aggcggctgc
      601 actgcttggc gtgcatcgct cgaccctgta ccgcgcactt gagcgcagcg aggaagtgac
      661 gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac gcattgaccg aggccgacgc
      721 cctggcggcc gccgagaatg aacgccaaga ggaacaagca tgaaaccgca ccaggacggc
      781 caggacgaac cgtttttcat taccgaagag atcgaggcgg agatgatcgc ggccgggtac
      841 gtgttcgagc cgcccgcgca cgtctcaacc gtgcggctgc atgaaatcct ggccggtttg
      901 tctgatgcca agctggcggc ctggccggcc agcttggccg ctgaagaaac cgagcgccgc
      961 cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat
     1021 atgatgcgat gagtaaataa acaaatacgc aaggggaacg catgaaggtt atcgctgtac
     1081 ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac ccatctagcc cgcgccctgc
     1141 aactcgccgg ggccgatgtt ctgttagtcg attccgatcc ccagggcagt gcccgcgatt
     1201 gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg
     1261 accgcgacgt gaaggccatc ggccggcgcg acttcgtagt gatcgacgga gcgccccagg
     1321 cggcggactt ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc
     1381 caagccctta cgacatatgg gccaccgccg acctggtgga gctggttaag cagcgcattg
     1441 aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc aaaggcacgc
     1501 gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt cttgagtccc
     1561 gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc gttcttgaat
     1621 cagaacccga gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa
     1681 aactcatttg agttaatgag gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc
     1741 cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc
     1801 agccatgaag cgggtcaact ttcagttgcc ggcggaggat cacaccaagc tgaagatgta
     1861 cgcggtacgc caaggcaaga ccattaccga gctgctatct gaatacatcg cgcagctacc
     1921 agagtaaatg agcaaatgaa taaatgagta gatgaatttt agcggctaaa ggaggcggca
     1981 tggaaaatca agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg
     2041 gcggttggcc aggcgtaagc ggctgggttg tctgccggcc ctgcaatggc actggaaccc
     2101 ccaagcccga ggaatcggcg tgacggtcgc aaaccatccg gcccggtaca aatcggcgcg
     2161 gcgctgggtg atgacctggt ggagaagttg aaggccgcgc aggccgccca gcggcaacgc
     2221 atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa
     2281 gaatcccggc aaccgccggc agccggtgcg ccgtcgatta ggaagccgcc caagggcgac
     2341 gagcaaccag attttttcgt tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc
     2401 atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc
     2461 cgctacgagc ttccagacgg gcacgtagag gtttccgcag ggccggccgg catggccagt
     2521 gtgtgggatt acgacctggt actgatggcg gtttcccatc taaccgaatc catgaaccga
     2581 taccgggaag ggaagggaga caagcccggc cgcgtgttcc gtccacacgt tgcggacgta
     2641 ctcaagttct gccggcgagc cgatggcgga aagcagaaag acgacctggt agaaacctgc
     2701 attcggttaa acaccacgca cgttgccatg cagcgtacga agaaggccaa gaacggccgc
     2761 ctggtgacgg tatccgaggg tgaagccttg attagccgct acaagatcgt aaagagcgaa
     2821 accgggcggc cggagtacat cgagatcgag ctagctgatt ggatgtaccg cgagatcaca
     2881 gaaggcaaga acccggacgt gctgacggtt caccccgatt actttttgat cgatcccggc
     2941 atcggccgtt ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga agccagatgg
     3001 ttgttcaaga cgatctacga acgcagtggc agcgccggag agttcaagaa gttctgtttc
     3061 accgtgcgca agctgatcgg gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg
     3121 gggcaggctg gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc
     3181 gccggttcct aatgtacgga gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt
     3241 cgaaaaggtg tctttcctgt ggatagcacg tacattggga acccaaagcc gtacattggg
     3301 aaccggaacc cgtacattgg gaacccaaag ccgtacattg ggaaccggtc acacatgtaa
     3361 gtgactgata taaaagagaa aaaaggcgat ttttccgcct aaaactcttt aaaacttatt
     3421 aaaactctta aaacccgcct ggcctgtgca taactgtctg gccagcgcac agccgaagag
     3481 ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg
     3541 cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg
     3601 cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc cacatcaagg caccctgcct
     3661 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     3721 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     3781 tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg
     3841 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
     3901 ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact
     3961 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     4021 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     4081 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     4141 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     4201 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     4261 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     4321 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     4381 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     4441 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     4501 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     4561 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     4621 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     4681 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     4741 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgcatga tatatctccc
     4801 aatttgtgta gggcttatta tgcacgctta aaaataataa aagcagactt gacctgatag
     4861 tttggctgtg agcaattatg tgcttagtgc atctaatcgc ttgagttaac gccggcgaag
     4921 cggcgtcggc ttgaacgaat ttctagctag acattatttg ccgactacct tggtgatctc
     4981 gcctttcacg tagtggacaa attcttccaa ctgatctgcg cgcgaggcca agcgatcttc
     5041 ttcttgtcca agataagcct gtctagcttc aagtatgacg ggctgatact gggccggcag
     5101 gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg attttgccgg ttactgcgct
     5161 gtaccaaatg cgggacaacg taagcactac atttcgctca tcgccagccc agtcgggcgg
     5221 cgagttccat agcgttaagg tttcatttag cgcctcaaat agatcctgtt caggaaccgg
     5281 atcaaagagt tcctccgccg ctggacctac caaggcaacg ctatgttctc ttgcttttgt
     5341 cagcaagata gccagatcaa tgtcgatcgt ggctggctcg aagatacctg caagaatgtc
     5401 attgcgctgc cattctccaa attgcagttc gcgcttagct ggataacgcc acggaatgat
     5461 gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga atctcgctct ctccagggga
     5521 agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat caagccttac
     5581 ggtcaccgta accagcaaat caatatcact gtgtggcttc aggccgccat ccactgcgga
     5641 gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga cgccaactac
     5701 ctctgatagt tgagtcgata cttcggcgat caccgcttcc cccatgatgt ttaactttgt
     5761 tttagggcga ctgccctgct gcgtaacatc gttgctgctc cataacatca aacatcgacc
     5821 cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata gactgtaccc caaaaaaaca
     5881 gtcataacaa gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt
     5941 tctggaccag ttgcgtgagc gcatacgcta cttgcattac agcttacgaa ccgaacaggc
     6001 ttatgtccac tgggttcgtg cccgaattga tcacaggcag caacgctctg tcatcgttac
     6061 aatcagcatg ctaccctccg cgagatcatc cgtgtttaaa acccggcagc ttagttgccg
     6121 ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg gctctcccgc
     6181 tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc
     6241 ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac aaattgacgc
     6301 ttagacaact taataacaca ttgcggatcc ctcggtcaca tgtgcatcct ctctgggaga
     6361 gcggagaatt aagggagtca cgttatgacc cccgccgatg acgcgggaca agccgtttta
     6421 cgtttggaac tgacagaacc gcaacgttga aggagccact cagccgcggg tttctggagt
     6481 ttaatgagct aagcacatac gtcagaaacc attattgcgc gttcaaaagt cgcctaaggt
     6541 cactatcagc tagcaaatat ttcttgtcaa aaatgctcca ctgacgttcc ataaattccc
     6601 ctcggtatcc aattagagtc tcatattcac tctcatactg tatttttaca acaattacca
     6661 acaacaacaa acaacaaaca acattacaat tactatttac aattacaatg aaaaagcctg
     6721 aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc
     6781 tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg
     6841 gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc
     6901 ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg gaattcagcg
     6961 agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg
     7021 aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg
     7081 ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca
     7141 ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg
     7201 tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg
     7261 ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc
     7321 tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt
     7381 cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc
     7441 agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt
     7501 atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg
     7561 atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg
     7621 ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac
     7681 tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa taggcttgtc
     7741 aagcagatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc
     7801 ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt
     7861 aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt
     7921 aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt
     7981 catctatgtt actagatcga cgctccatgg gaattcgtaa agcgactaaa tggagcaacc
     8041 tactgttttt gaagtttggg ttgccatgta atcacttgca ataaatatat gtactaatga
     8101 ctgcatcggc ttgcgtgtct aaatttgtag cttattgaaa actttttgtg gttaaagacg
     8161 aatccagtta actaacataa gtttaacaaa acaagacctt ttccatatat agaccaccgt
     8221 taccaactta caaagaagcc aaataaagta caacacaaca cttgcaagtt acaaaaagag
     8281 aggaaaaaaa acatggtacc atttgacaac atatagaaac acacataaac attacttata
     8341 acaaaaagat tcaaactcgg cttttattat ctccccctga tcaaattcta aacactttat
     8401 tcctcataaa ctaactcccc gacattggag ttattggact atgacgatga catcagtggg
     8461 gtaagctcac ttgtgcccca gtttgctagg gaggtcgcag tatctggcca cagccacctc
     8521 gtgctgctcg acgtaggttt ctttgtcggc ctccttgatt ctttccagtc tgtggtccac
     8581 atagtagacg ccgggcatct tgaggttctt agcgggtttc ttggatctgt atgtggtctt
     8641 gaagttgcag atcaggtggc ccccgcccac gagcttcagg gccatgtcgc ttctgccttc
     8701 caggccgccg tcagcggggt acagcatctc ggtgttggcc tcccagccga gtgttttctt
     8761 ctgcatcaca gggccgttgg atgggaagtt cacccctctg atcttgacgt tgtagatgag
     8821 gcagccgtcc tggaggctgg tgtcctgggt agcggtcagc acgcccccat cttcgtatgt
     8881 ggtgactctc tcccatgtga agccctcagg gaaggactgc ttaaagaagt cggggatgcc
     8941 ctgggtgtgg ttgatgaagg ttctgctgcc gtacatgaag ctggtagcca ggatgtcgaa
     9001 ggcgaagggg agagggccgc cctcgaccac cttgattctc atggtctggg tgccctcgta
     9061 gggcttgcct tcgccctcgg atgtgcactt gaagtggtgg ttgttcacgg tgccctccat
     9121 gtacagcttc atgtgcatgt tctccttaat cagctcgctc atagtagtgt gctggccacc
     9181 acgagtacgg tcacggtcat gttccccacc agtatgttgc tgtccgtagt actgagctct
     9241 gtctttcaga tcctgagctt tgcttcccaa cttcatcctt gcactgtcca acttgtctga
     9301 tccctgtggg tgctctcccg ttgcgtacct attcacaatc aataggtatc ataccaagca
     9361 ttttacctat atagatgata tacacagaca cattcaagct agctaggtca aatttcgtat
     9421 ttagttaacc aaatgttccc tagaatttgc aatttcttat tgttacattt gttattcgac
     9481 caaaaaaatt gatccaaagc tactggctca acacatgcat gcacatattg cacaaaatta
     9541 tgaagtaaga tgataaatgt gtgcttactt gtaaatccaa gagaaaacgg ttatagcggc
     9601 aatgccaaac cctccagagg aaagaaaacc ggtgatgagg agtgcaactg tgatgagagc
     9661 cgggacaagg attgggctga agataacgag cagaggtgtt gcaacagtca aagctatgac
     9721 agttccaaca agggtaaggc tggagagaac aaggagggaa ccaccagctg tgacagcagt
     9781 tgcagcttta gcaatctgcc tagacttgga gtagtcagat cctcgtccgg acatctggta
     9841 ctggtcacga tctcggccca tcatcgggta ctgatctctg ccgatgatat cgtgatgggt
     9901 tcctctagct gtatccgcca tttttttgtt cttgtttact agagagaatg agattttgaa
     9961 tgagagattg atcaaaaaga tgtgtatata ttttggaaga ggtaggagtg tttgttagat
    10021 atattaagaa aggagacgag aagtcacgtg tcaatggaga catcatgcaa ttgtaggcca
    10081 cgtgttaatg gcgtcaccat gcagccgctg catgtgttaa cgagaggagt ctattcaaag
    10141 acacgtttgt tttttgccta gctcagaatg tggcgttggc atcgaaccga aacataaaac
    10201 gcaaaacgaa cctttgggct cgtcagtatg ggtttgggcc ttcaatagat tccttctata
    10261 cggcccacat atccactatt ggcaacaaaa ttagaaaaat ttaggattta gttttaaaat
    10321 ttgttccgcg acattctaga actccgagct cgaattctag tagcgtcgat ctagtaacat
    10381 agatgacacc gcgcgcgata atttatccta gtttgcgcgc tatattttgt tttctatcgc
    10441 gtattaaatg tataattgcg ggactctaat cataaaaacc catctcataa ataacgtcat
    10501 gcattacatg ttaattatta catgcttaac gtaattcaac agaaattata tgataatcat
    10561 cgcaagaccg gcaacaggat tcaatcttaa gaaactttat tgccaaatgt ttgaacgatc
    10621 tgcttgacaa gctcacacct tcctcttctt cttggggtca gccctgctgt ctccaccgag
    10681 ctgagagagg tcgattcttg tttcatagag ccccgtaatt gactgatgaa tcagtgtggc
    10741 gtccaggacc tcctttgtag aggtgtaccg ctttctgtct atggtggtgt cgaagtactt
    10801 gaaggctgca ggcgcgccca agttggtcag agtaaacaag tggataatgt tttctgcctg
    10861 ctccctgatg ggcttatccc tgtgcttatt gtaagcagaa agcaccttat cgaggttagc
    10921 gtcggcgagg atcactcttt tggagaattc gcttatttgc tcgatgatct catcaaggta
    10981 gtgtttgtgt tgttccacga acagctgctt ctgctcatta tcttcgggag atcctttgag
    11041 cttttcatag tggctggcca gatacaagaa attaacgtat ttagagggca gtgccagctc
    11101 gttacctttc tgcagctcgc ccgcactagc gagcattcgt ttccggccgt tttcaagctc
    11161 aaagagagag tacttgggaa gcttaatgat gaggtctttt ttgacctctt tatatccttt
    11221 cgcctcgaga aagtcgatgg ggtttttttc gaagcttgat cgctccatga ttgtgatgcc
    11281 cagcagttcc ttgacgcttt tgagtttttt agacttccct ttctccactt tggccacaac
    11341 cagtacactg taagcgactg taggagaatc gaatccgccg tatttcttgg ggtcccaatc
    11401 ttttttgcgt gcgatcagct tgtcgctgtt ccttttcggg aggatacttt ccttggagaa
    11461 gcctccggtc tgtacttcgg tctttttaac gatgttcacc tgcggcatgg acaggacctt
    11521 ccggactgtc gcgaaatccc tacccttgtc ccacacgatt tctcctgttt ctccgtttgt
    11581 ttcgataagt ggtcgcttcc gaatctctcc attggccagt gtaatctcgg tcttgaaaaa
    11641 attcataata ttgctgtaaa agaagtactt agcggtggcc ttgcctattt cctgctcaga
    11701 ctttgcgatc attttcctaa catcgtacac tttatagtct ccgtaaacaa attcagattc
    11761 aagcttggga tattttttga taagtgcagt gcctaccact gcattcaggt aggcatcatg
    11821 cgcatggtgg taattgttga tctctctcac cttataaaac tgaaagtcct ttctgaaatc
    11881 tgaaaccagc ttagacttca gagtaataac tttcacctct cgaatcagtt tgtcattttc
    11941 atcgtacttg gtgttcatgc gtgaatcgag aatttgggcc acgtgcttgg tgatctggcg
    12001 tgtctcaaca agctgccttt tgatgaagcc ggctttatcc aactcagaca ggccacctcg
    12061 ttcagcctta gtcagattat cgaacttccg ttgtgtgatc agtttggcgt tcagcagctg
    12121 ccgccaataa tttttcattt tcttgacaac ttcttctgag gggacgttat cactcttccc
    12181 tctattttta tcggatcttg tcaacacttt attatcaata gaatcatctt tgagaaaaga
    12241 ctggggcacg atatgatcca cgtcgtagtc ggagagccga ttgatgtcca gttcctgatc
    12301 cacgtacatg tccctgccgt tctgcaggta gtacaggtag agcttctcat tctgaagctg
    12361 ggtgttttca actgggtgtt ccttaaggat ttgggacccc agttctttta taccctcttc
    12421 aatcctcttc atcctttccc tactgttctt ctgtcccttc tgggtagttt ggttctctcg
    12481 ggccatctcg ataacgatat tctcgggctt atgccttccc attactttga cgagttcatc
    12541 cacgacctta acggtctgca gtattccctt tttgatagct gggctacctg caagattagc
    12601 gatgtgctcg tggagactgt ccccctggcc agaaacttgt gctttctgga tgtcctcctt
    12661 aaaggtgaga gagtcatcat ggatcaactg catgaagttc cggttggcaa atccatcgga
    12721 cttaagaaaa tccaggattg tctttccact ctgcttgtct cggatcccat tgatcagttt
    12781 tcttgacagc cgcccccatc ctgtatatcg gcgcctcttg agctgtttca tgactttgtc
    12841 gtcgaagaga tgagcgtaag ttttcaagcg ttcttcaatc atctccctat cttcaaacaa
    12901 cgtaagggtg aggacaatgt cctcaagaat gtcctcgttc tcctcattgt ccaggaagtc
    12961 cttgtcttta atgattttca ggagatcgtg atacgttccc agggatgcgt tgaagcgatc
    13021 ctccactccg ctgatttcaa cagagtcgaa acattcaatc tttttgaaat aatcttcttt
    13081 gagctgtttc acggtaactt tccggttcgt cttgaagagg aggtccacga tagctttctt
    13141 ctgctctcca gacaggaatg ctggctttct catcccttct gtgacgtatt tgaccttggt
    13201 gagctcgtta taaactgtga agtactcgta cagcagagag tgtttaggaa gcaccttttc
    13261 gttaggcaga tttttatcaa agttagtcat cctttcgatg aaggactggg cagaggcccc
    13321 cttatccacg acttcctcga agttccaggg agtgatagtc tcttctgatt tgcgagtcat
    13381 ccacgcgaat ctggaatttc cccgggcgag ggggcctaca tagtagggta tccgaaatgt
    13441 gaggattttc tcaatctttt ccctgttatc tttcaaaaag gggtagaaat cctcttgccg
    13501 cctgaggata gcgtgcagtt cgcccaggtg aatctggtgg gggatgcttc cattgtcgaa
    13561 agtgcgctgt ttgcgcaaca gatcttctct gttaagcttt accagcagct cctcggtgcc
    13621 gtccattttt tccaagatgg gcttaataaa tttgtaaaat tcctcctggc ttgctccgcc
    13681 gtcaatgtat ccggcgtagc catttttaga ctgatcgaag aaaatttcct tgtacttctc
    13741 aggcagttgc tgtctgacaa gggccttcag caaagtcaag tcttggtggt gctcatcata
    13801 gcgcttgatc atactagcgc tcagcggagc tttggtgatc tccgtgttca ctcgcagaat
    13861 atcactcagc agaatggcgt ctgacaggtt ctttgccgcc aaaaaaaggt ctgcgtactg
    13921 gtcgccgatc tgggccagca gattgtcgag atcatcatcg taggtgtctt tgctcagttg
    13981 aagcttggca tcttcggcca ggtcgaagtt agatttaaag ttgggggtca gcccgagtga
    14041 cagggcgata agattaccaa acaggccgtt cttcttctcc ccagggagct gtgcgatgag
    14101 gttttcgagc cgccgggatt tggacagcct agcgctcagg attgctttgg cgtcaactcc
    14161 ggatgcgttg atcgggttct cttcgaaaag ctgattgtaa gtctgaacca gttggataaa
    14221 gagtttgtcg acatcgctgt tgtctgggtt caggtccccc tcgatgagga agtgtccccg
    14281 aaatttgatc atatgcgcca gcgcgagata gatcaaccgc aagtcagcct tatcagtact
    14341 gtctacaagc ttcttcctca gatgatatat ggttgggtac ttttcatggt acgccacctc
    14401 gtccacgata ttgccaaaga ttgggtggcg ctcgtgcttt ttatcctcct ccaccaaaaa
    14461 ggactcctcc agcctatgga agaaagagtc atccacctta gccatctcat tactaaagat
    14521 ctcctgcagg tagcagatcc gattctttct gcgggtatat ctgcgccgtg ctgttctttt
    14581 gagccgcgtg gcttcggccg tctccccgga gtcgaacagg agggcgccaa tgaggttctt
    14641 ctttatgctg tggcgatcgg tattgcccag aactttgaat tttttgctcg gcaccttgta
    14701 ctcgtccgta atgacggccc agccgacgct gtttgtgccg atatcgagcc caatggagta
    14761 cttcttgtcc attgtatcga taattgtaaa tgtaattgta atgttgtttg ttgtttgttg
    14821 ttgttggtaa ttgttgtaaa aatgagctct tatactcgag cgtgtcctct ccaaatgaaa
    14881 tgaacttcct tatatagagg aagggtcttg cgaaggatag tgggattgtg cgtcatccct
    14941 tacgtcagtg gagatgtcac atcaatccac ttgctttgta gacgtggttg gaacctcttc
    15001 tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt cggcagagag
    15061 atcttgaatg atagcctttc ctttatcgca atgatggcat ttgtaggagc caccttcctt
    15121 ttctactgtc ctttcgatga agtgacagat agctgggcaa tggaatccga ggaggtttcc
    15181 cgaaattatc ctttgttgaa aagtctcaat agccctttga tcttctgaga ctgtatcttt
    15241 gacatttttg gagtagacca gagtgtcgtg ctccaccatg ttatcacatc aatccacttg
    15301 ctttgtagac gtggttggaa cctcttcttt ttccacgatg ctcctcgtgg gtgggggtcc
    15361 atctttggga ccactgtcgg cagagagatc ttgaatgata gcctttcctt tatcgcaatg
    15421 atggcatttg taggagccac cttccttttc tactgtcctt tcgatgaagt gacagatagc
    15481 tgggcaatgg aatccgagga ggtttcccga aattatcctt tgttgaaaag tctcaatagc
    15541 cctttgatct tctgagactg tatctttgac atttttggag tagaccagag tgtcgtgctc
    15601 caccatgttg acctccaagc ttgaattctt gcagagaccg agacgttgat cggcacgtaa
    15661 gaggttccaa ctttcaccat aatgaaataa gatcactacc gggcgtattt tttgagttat
    15721 cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg
    15781 ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat
    15841 gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa
    15901 ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc
    15961 cggaattccg tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt
    16021 gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg
    16081 acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc
    16141 tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg
    16201 tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt
    16261 tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg
    16321 ttcatcatgc cgtctgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt
    16381 actgcgatga gtggcagggc ggggcgtaat cgcgtggatc cggcttacta aaagccagat
    16441 aacagtatgc gtatttgcgc gctgattttt gcggtataag aatatatact gatatgtata
    16501 cccgaagtat gtcaaaaaga ggtatgctat gaagcagcgt attacagtga cagttgacag
    16561 cgacagctat cagttgctca aggcatatat gatgtcaata tctccggtct ggtaagcaca
    16621 accatgcaga atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa
    16681 gggatggctg aggtcgcccg gtttattgaa atgaacggct cttttgctga cgagaacagg
    16741 ggctggtgaa atgcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt
    16801 tgtggatgta cagagtgata ttattgacac gcccgggcga cggatggtga tccccctggc
    16861 cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg
    16921 ggatgaaagc tggcgcatga tgaccaccga tatggccagt gtgccggttt ccgttatcgg
    16981 ggaagaagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat
    17041 gttctgggga atataaatgt caggctccct tatacacagc cagtcggtct cagcttctgc
    17101 aggcatgcga cgtcgagctt agcttgagct tggatcagat tgtcgtttcc cgccttcagt
    17161 ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt
    17221 attagaataa tcggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat
    17281 gtgcatgcca accacagggt tcccctcggg atcaaagtac tttaaagtac tttaaagtac
    17341 tttaaagtac tttgatccaa cccctccgct gctatagtgc agtcggcttc tgacgttcag
    17401 tgcagccgtg ttctgaaaac gacatgtcgc acaagtccta agttacgcga caggctgccg
    17461 ccctgccctt
//
