LOCUS Exported 12459 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Third generation Self-inactivating lentivector expressing NS5 from Zika virus. ACCESSION . VERSION . KEYWORDS pLV_Zika_NS5_Flag SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12459) TITLE Arumugaswami Lab Plasmids REFERENCE 2 (bases 1 to 12459) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12459 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 132..330 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 285..305 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 348..528 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 575..700 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1193..1426 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1611..1655 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 1953..2070 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2148..2359 /label=EF-1-alpha core promoter /note="core promoter for human elongation factor EF-1-alpha" LTR 2372..2640 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" CDS complement(3484..3495) /codon_start=1 /product="WELQut protease recognition and cleavage site" /label=WELQut site /translation="WELQ" CDS 5397..5420 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" misc_feature 5469..6041 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(5636..5653) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 5863..5882 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 6044..6646 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind 6543..6563 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 6662..7250 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(6715..6735) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(7133..7144) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 7777..7957 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(7983..8000) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 7989..8213 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 8259..8687 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(8346..8365) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 8556..8577 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(8696..8716) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 8701..9030 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 8881..9016 /label=SV40 ori /note="SV40 origin of replication" primer_bind 8943..8962 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 9078..9125 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 9144..9518 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 9648..9769 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(9685..9704) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 9739..9758 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(9818..9834) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(9818..9834) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(9831..9853) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 9842..9858 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(9866..9896) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9911..9932 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(10049..10066) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(10220..10808) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(10300..10319) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(10979..11839) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 11602..11621 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(11840..11944) /gene="bla" /label=AmpR promoter primer_bind complement(12019..12038) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 12210..130 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" ORIGIN 1 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc 61 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc 121 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc 181 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa 241 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag 301 gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg tctctctggt 361 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc 421 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta 481 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa 541 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc 601 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac 661 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat 721 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa 781 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga 841 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc 901 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat 961 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa 1021 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca 1081 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac 1141 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt 1201 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga 1261 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg 1321 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg 1381 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt 1441 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat 1501 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt 1561 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac 1621 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt 1681 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag 1741 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc 1801 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg 1861 gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa 1921 ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg 1981 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta 2041 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca 2101 gtttggttaa ttctagtgga tctgcgatcg ctccggtgcc cgtcagtggg cagagcgcac 2161 atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaacgg gtgcctagag 2221 aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga 2281 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg 2341 gtttgccgcc agaacacagc tgaagcttcg aggggctcgc atctctcctt cacgcgcccg 2401 ccgccctacc tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg 2461 tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg 2521 cctttgtccg gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct 2581 gaccctgctt gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc 2641 caagctgtga ccggcgccta ccaccggtca ccatgggagg tggaacagga gagaccctgg 2701 gagagaaatg gaaggcccgc ttgaaccaga tgtcggccct ggagttctac tcctacaaaa 2761 agtcaggcat caccgaggtg tgcagagaag aggcccgccg cgccctcaag gacggtgtgg 2821 caacgggagg ccatgctgtg tcccgaggaa gtgcaaagct gagatggttg gtggagcggg 2881 gatacctgca gccctatgga aaggtcattg atcttggatg tggcagaggg ggctggagtt 2941 actacgtcgc caccatccgc aaagttcaag aagtgaaagg atacacaaaa ggaggccctg 3001 gtcatgaaga acccgtgttg gtgcaaagct atgggtggaa catagtccgt cttaagagtg 3061 gggtggacgt ctttcatatg gcggctgagc cgtgtgacac gttgctgtgt gacataggtg 3121 agtcatcatc tagtcctgaa gtggaagaag cacggacgct cagagtcctc tccatggtgg 3181 gggattggct tgaaaaaaga ccaggagcct tttgtataaa agtgttgtgc ccatacacca 3241 gcactatgat ggaaaccctg gagcgactgc agcgtaggta tgggggagga ctggtcagag 3301 tgccactctc ccgcaactct acacatgaga tgtactgggt ctctggagcg aaaagcaaca 3361 ccataaaaag tgtgtccacc acgagccagc tcctcttggg gcgcatggac gggcctagga 3421 ggccagtgaa atatgaggag gatgtgaatc tcggctctgg cacgcgggct gtggtaagct 3481 gcgctgaagc tcccaacatg aagatcattg gtaaccgcat tgaaaggatc cgcagtgagc 3541 acgcggaaac gtggttcttt gacgagaacc acccatatag gacatgggct taccatggaa 3601 gctatgaggc ccccacacaa gggtcagcgt cctctctaat aaacggggtt gtcaggctcc 3661 tgtcaaaacc ctgggatgtg gtgactggag tcacaggaat agccatgacc gacaccacac 3721 cgtatggtca gcaaagagtt ttcaaggaaa aagtggacac tagggtgcca gacccccaag 3781 aaggcactcg tcaggttatg agcatggtct cttcctggtt gtggaaagag ctaggcaaac 3841 acaaacggcc acgagtctgc accaaagaag agttcatcaa caaggttcgt agcaatgcag 3901 cattaggggc aatatttgaa gaggaaaaag agtggaagac tgcagtggaa gctgtgaacg 3961 atccaaggtt ctgggctcta gtggacaagg aaagagagca ccacctgaga ggagagtgcc 4021 agagctgtgt gtacaacatg atgggaaaaa gagaaaagaa acaaggggaa tttggaaagg 4081 ccaagggcag ccgcgccatc tggtatatgt ggctaggggc tagatttcta gagttcgaag 4141 cccttggatt cttgaacgag gatcactgga tggggagaga gaactcagga ggtggtgttg 4201 aagggctggg attacaaaga ctcggatatg tcctagaaga gatgagtcgt ataccaggag 4261 gaaggatgta tgcagatgac actgctggct gggacacccg cattagcagg tttgatctgg 4321 agaatgaagc tctaatcacc aaccaaatgg agaaagggca cagggccttg gcattggcca 4381 taatcaagta cacataccaa aacaaagtgg taaaggtcct tagaccagct gaaaaaggga 4441 aaacagttat ggacattatt tcgagacaag accaaagggg gagcggacaa gttgtcactt 4501 acgctcttaa cacatttacc aacctagtgg tgcaactcat tcggaatatg gaggctgagg 4561 aagttctaga gatgcaagac ttgtggctgc tgcggaggtc agagaaagtg accaactggt 4621 tgcagagcaa cggatgggat aggctcaaac gaatggcagt cagtggagat gattgcgttg 4681 tgaagccaat tgatgatagg tttgcacatg ccctcaggtt cttgaatgat atgggaaaag 4741 ttaggaagga cacacaagag tggaaaccct caactggatg ggacaactgg gaagaagttc 4801 cgttttgctc ccaccacttc aacaagctcc atctcaagga cgggaggtcc attgtggttc 4861 cctgccgcca ccaagatgaa ctgattggcc gggcccgcgt ctctccaggg gcgggatgga 4921 gcatccggga gactgcttgc ctagcaaaat catatgcgca aatgtggcag ctcctttatt 4981 tccacagaag ggacctccga ctgatggcca atgccatttg ttcatctgtg ccagttgact 5041 gggttccaac tgggagaact acctggtcaa tccatggaaa gggagaatgg atgaccactg 5101 aagacatgct tgtggtgtgg aacagagtgt ggattgagga gaacgaccac atggaagaca 5161 agaccccagt tacgaaatgg acagacattc cctatttggg aaaaagggaa gacttgtggt 5221 gtggatctct catagggcac agaccgcgca ccacctgggc tgagaacatt aaaaacacag 5281 tcaacatggt gcgcaggatc ataggtgatg aagaaaagta catggactac ctatccaccc 5341 aagttcgcta cttgggtgaa gaagggtcta cacctggagt gctgtccggg atggccgact 5401 acaaagacga cgatgacaag tagcactcga ggatatcgac gcgtgaagct tagcggccgc 5461 aaattccgcc cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa 5521 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg 5581 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc 5641 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct 5701 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac 5761 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc 5821 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta 5881 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg 5941 cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg 6001 aaccacgggg acgtggtttt cctttgaaaa acacgataat accatggcca ccgagtacaa 6061 gcccacggtg cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca ccctcgccgc 6121 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgac ccggaccgcc acatcgagcg 6181 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg 6241 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg 6301 ggcggtgttc gccgagatcg gctcgcgcat ggccgagttg agcggttccc ggctggccgc 6361 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct 6421 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct 6481 ccccggagtg gaggcggccg agcgcgctgg ggtgcccgcc ttcctggaga cctccgcgcc 6541 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc 6601 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgagttc gcgtctggaa 6661 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc 6721 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg 6781 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt 6841 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac 6901 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc 6961 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct 7021 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct 7081 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct 7141 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct 7201 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattaa 7261 ttctgcagtc gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc 7321 aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca 7381 cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta 7441 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat 7501 ctgtggatct accacacaca aggctacttc cctgattggc agaactacac accagggcca 7561 gggatcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagcaagag 7621 aaggtagaag aagccaatga aggagagaac acccgcttgt tacaccctgt gagcctgcat 7681 gggatggatg acccggagag agaagtatta gagtggaggt ttgacagccg cctagcattt 7741 catcacatgg cccgagagct gcatccggac tgtactgggt ctctctggtt agaccagatc 7801 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg 7861 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc 7921 ctcagaccct tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa cccgctgatc 7981 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc 8041 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc 8101 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg 8161 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga 8221 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt 8281 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc 8341 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca 8401 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc 8461 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt 8521 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac 8581 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc 8641 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat 8701 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc 8761 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga 8821 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc 8881 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 8941 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga 9001 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc 9061 ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat 9121 acgacaaggt gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg 9181 cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact 9241 tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg 9301 tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg 9361 agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg 9421 ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg 9481 gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt 9541 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga 9601 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg 9661 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt 9721 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta 9781 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa 9841 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct 9901 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc 9961 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg 10021 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 10081 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 10141 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 10201 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 10261 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 10321 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 10381 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 10441 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 10501 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 10561 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 10621 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg 10681 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 10741 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 10801 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 10861 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 10921 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 10981 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 11041 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 11101 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 11161 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 11221 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 11281 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 11341 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 11401 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 11461 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 11521 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 11581 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 11641 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 11701 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 11761 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 11821 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 11881 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 11941 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc 12001 cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct 12061 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag 12121 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct 12181 tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta 12241 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac 12301 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac 12361 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt 12421 acggtaaact gcccacttgg cagtacatca agtgtatca //