LOCUS       Exported                1230 bp ds-DNA     linear   SYN 20-JUN-2021
DEFINITION  This plasmid encodes the kinase domain of DMPK. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    DMPK_HUMAN_D0
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 1230)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 20, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     RBS             153..161
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 cagcagctgg tgttggaccc gggcttcctg gggctggagc ccctgctcga ccttctcctg
       61 ggcgtccacc aggagctggg cgcctccgaa ctggcccagg acaagtacgt ggccgacttc
      121 ttgcagtggg cggagcccat cgtggtgagg cttaaggagg tccgactgca gagggacgac
      181 ttcgagattc tgaaggtgat cggacgcggg gcgttcagcg aggtagcggt agtgaagatg
      241 aagcagacgg gccaggtgta tgccatgaag atcatgaaca agtgggacat gctgaagagg
      301 ggcgaggtgt cgtgcttccg tgaggagagg gacgtgttgg tgaatgggga ccggcggtgg
      361 atcacgcagc tgcacttcgc cttccaggat gagaactacc tgtacctggt catggagtat
      421 tacgtgggcg gggacctgct gacactgctg agcaagtttg gggagcggat tccggccgag
      481 atggcgcgct tctacctggc ggagattgtc atggccatag actcggtgca ccggcttggc
      541 tacgtgcaca gggacatcaa acccgacaac atcctgctgg accgctgtgg ccacatccgc
      601 ctggccgact tcggctcttg cctcaagctg cgggcagatg gaacggtgcg gtcgctggtg
      661 gctgtgggca ccccagacta cctgtccccc gagatcctgc aggctgtggg cggtgggcct
      721 gggacaggca gctacgggcc cgagtgtgac tggtgggcgc tgggtgtatt cgcctatgaa
      781 atgttctatg ggcagacgcc cttctacgcg gattccacgg cggagaccta tggcaagatc
      841 gtccactaca aggagcacct ctctctgccg ctggtggacg aaggggtccc tgaggaggct
      901 cgagacttca ttcagcggtt gctgtgtccc ccggagacac ggctgggccg gggtggagca
      961 ggcgacttcc ggacacatcc cttcttcttt ggcctcgact gggatggtct ccgggacagc
     1021 gtgcccccct ttacaccgga tttcgaaggt gccaccgaca catgcaactt cgacttggtg
     1081 gaggacgggc tcactgccat ggtgagcggg ggcggggaga cactgtcgga cattcgggaa
     1141 ggtgcgccgc taggggtcca cctgcctttt gtgggctact cctactcctg catggccctc
     1201 agggacagtg aggtcccagg ccccacaccc
//
