LOCUS       Exported                 801 bp ds-DNA     linear   SYN 05-JUN-2021
DEFINITION  This plasmid encodes the kinase domain of AURKA. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    AURKA_HUMAN_D0
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 5, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             529..540
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 aagaggcagt gggctttgga agactttgaa attggtcgcc ctctgggtaa aggaaagttt
       61 ggtaatgttt atttggcaag agaaaagcaa agcaagttta ttctggctct taaagtgtta
      121 tttaaagctc agctggagaa agccggagtg gagcatcagc tcagaagaga agtagaaata
      181 cagtcccacc ttcggcatcc taatattctt agactgtatg gttatttcca tgatgctacc
      241 agagtctacc taattctgga atatgcacca cttggaacag tttatagaga acttcagaaa
      301 ctttcaaagt ttgatgagca gagaactgct acttatataa cagaattggc aaatgccctg
      361 tcttactgtc attcgaagag agttattcat agagacatta agccagagaa cttacttctt
      421 ggatcagctg gagagcttaa aattgcagat tttgggtggt cagtacatgc tccatcttcc
      481 aggaggacca ctctctgtgg caccctggac tacctgcccc ctgaaatgat tgaaggtcgg
      541 atgcatgatg agaaggtgga tctctggagc cttggagttc tttgctatga atttttagtt
      601 gggaagcctc cttttgaggc aaacacatac caagagacct acaaaagaat atcacgggtt
      661 gaattcacat tccctgactt tgtaacagag ggagccaggg acctcatttc aagactgttg
      721 aagcataatc ccagccagag gccaatgctc agagaagtac ttgaacaccc ctggatcaca
      781 gcaaattcat caaaaccatc a
//
