LOCUS Exported 811 bp ds-DNA linear SYN 13-JUN-2021 DEFINITION To overexpress TIP60 365-513 in Mammalian Cells. ACCESSION . VERSION . KEYWORDS p3xFLAG-CMV TIP60 365-513 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 811) AUTHORS Lee MS, Seo J, Choi DY, Lee EW, Ko A, Ha NC, Yoon JB, Lee HW, Kim KP, Song J TITLE Stabilization of p21 (Cip1/WAF1) following Tip60-dependent acetylation is required for p21-mediated DNA damage response. JOURNAL Cell Death Differ. 2013 Apr;20(4):620-9. doi: 10.1038/cdd.2012.159. Epub 2012 Dec 14. PUBMED 23238566 REFERENCE 2 (bases 1 to 811) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..811 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(649..714) /codon_start=1 /product="three tandem FLAG(R) epitope tags, followed by an enterokinase cleavage site" /label=3xFLAG /translation="DYKDHDGDYKDHDIDYKDDDDK" promoter complement(744..796) /label=CMVd2 promoter /note="truncated human cytomegalovirus (CMV) immediate early promoter that yields moderately reduced expression relative to the full-length promoter" ORIGIN 1 acttgcccct tgctccatac cacccccctc caccccataa tattatagaa ggacacctag 61 tcagacaaaa tgatgcaact taattttatt aggacaaggc tggtgggcac tggagtggca 121 acttccaggg ccaggagagg cactggggag gggtcacagg gatgccaccc gggatcctct 181 agatcaccac ttccccctct tgctccagtc cttgggagtg aagtgcagac acttggagtc 241 gatccgcagg agccgcttga gcatggcccg ctcatggcca tccacgatgt cctctgacag 301 tgtgaggatg tactggccct tgtagtagtt gatgagattg aggtactgca gagtggagat 361 gacatcctcc ttcttgatgc tggtgatttc actaatctca ttgatggtga tctgtggcct 421 ctccccgctc tccgacttca gccccatcag gatctccagg atggtctggg accagtagct 481 tcgataggat aggaggccaa ggtctgagag gggcttctca ggggtccctg ttttcccttc 541 cactttggag agttcatagc tgaactcgat cagcagcttg ccgtagcccc ggcgctggta 601 gggaggcagg gttaggatgc aggccacatt gtacattgcg gccgcaagct tgtcatcgtc 661 atccttgtaa tcgatgtcat gatctttata atcaccgtca tggtctttgt agtccatggt 721 taattctgac ggttcactaa acgagctctg cttatataga cctcccaccg tacacgccta 781 cccgcccatt tgcgtcaacg ggggcggggg t //