LOCUS       Exported               13220 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  genomic Sindbis plasmid, encoding MAPPnL and a GFP RNA with a 
            barcode landing pad.
ACCESSION   .
VERSION     .
KEYWORDS    jk100 pSinEGdsp(p1:MAPPnL;p2:gfp:barcodelandingpad:4xboxB)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13220)
  TITLE     Zador Lab MAPseq plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13220)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13220
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             7753..7800
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             7837..7866
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             7873..8418
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylcytosine 
                     derivatives"
                     /label=CLIP-tag(TM)
                     /note="optimized for mammalian expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLIQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISESHLAALVGNPAATAAVNTALDGNPVPILIPCHRVVQGDSDVGPYLGGLA
                     VKEWLLAHEGHRLGKPGLG"
     regulatory      9187..9196
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             9193..9258
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=-lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     regulatory      9316..9325
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             9322..9387
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=-lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     regulatory      9445..9454
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             9451..9516
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=-lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     regulatory      9574..9583
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             9580..9645
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=-lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     regulatory      9976..9985
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             9982..10701
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             9982..10698
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(10027..10048)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(10288..10307)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     10635..10656
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        11391..11495
                     /gene="bla"
                     /label=AmpR promoter
     CDS             11496..12356
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11714..11733)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12527..13115
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13016..13035
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 attgacggcg tagtacacac tattgaatca aacagccgac caattgcact accatcacaa
       61 tggagaagcc agtagtaaac gtagacgtag acccccagag tccgtttgtc gtgcaactgc
      121 aaaaaagctt cccgcaattt gaggtagtag cacagcaggt cactccaaat gaccatgcta
      181 atgccagagc attttcgcat ctggccagta aactaatcga gctggaggtt cctaccacag
      241 cgacgatctt ggacataggc agcgcaccgg ctcgtagaat gttttccgag caccagtatc
      301 attgtgtctg ccccatgcgt agtccagaag acccggaccg catgatgaaa tacgccagta
      361 aactggcgga aaaagcgtgc aagattacaa acaagaactt gcatgagaag attaaggatc
      421 tccggaccgt acttgatacg ccggatgctg aaacaccatc gctctgcttt cacaacgatg
      481 ttacctgcaa catgcgtgcc gaatattccg tcatgcagga cgtgtatatc aacgctcccg
      541 gaactatcta tcatcaggct atgaaaggcg tgcggaccct gtactggatt ggcttcgaca
      601 ccacccagtt catgttctcg gctatggcag gttcgtaccc tgcgtacaac accaactggg
      661 ccgacgagaa agtccttgaa gcgcgtaaca tcggactttg cagcacaaag ctgagtgaag
      721 gtaggacagg aaaattgtcg ataatgagga agaaggagtt gaagcccggg tcgcgggttt
      781 atttctccgt aggatcgaca ctttatccag aacacagagc cagcttgcag agctggcatc
      841 ttccatcggt gttccacttg aatggaaagc agtcgtacac ttgccgctgt gatacagtgg
      901 tgagttgcga aggctacgta gtgaagaaaa tcaccatcag tcccgggatc acgggagaaa
      961 ccgtgggata cgcggttaca cacaatagcg agggcttctt gctatgcaaa gttactgaca
     1021 cagtaaaagg agaacgggta tcgttccctg tgtgcacgta catcccggcc accatatgcg
     1081 atcagatgac tggtataatg gccacggata tatcacctga cgatgcacaa aaacttctgg
     1141 ttgggctcaa ccagcgaatt gtcattaacg gtaggactaa caggaacacc aacaccatgc
     1201 aaaattacct tctgccgatc atagcacaag ggttcagcaa atgggctaag gagcgcaagg
     1261 atgatcttga taacgagaaa atgctgggta ctagagaacg caagcttacg tatggctgct
     1321 tgtgggcgtt tcgcactaag aaagtacatt cgttttatcg cccacctgga acgcagacct
     1381 gcgtaaaagt cccagcctct tttagcgctt ttcccatgtc gtccgtatgg acgacctctt
     1441 tgcccatgtc gctgaggcag aaattgaaac tggcattgca accaaagaag gaggaaaaac
     1501 tgctgcaggt ctcggaggaa ttagtcatgg aggccaaggc tgcttttgag gatgctcagg
     1561 aggaagccag agcggagaag ctccgagaag cacttccacc attagtggca gacaaaggca
     1621 tcgaggcagc cgcagaagtt gtctgcgaag tggaggggct ccaggcggac atcggagcag
     1681 cattagttga aaccccgcgc ggtcacgtaa ggataatacc tcaagcaaat gaccgtatga
     1741 tcggacagta tatcgttgtc tcgccaaact ctgtgctgaa gaatgccaaa ctcgcaccag
     1801 cgcacccgct agcagatcag gttaagatca taacacactc cggaagatca ggaaggtacg
     1861 cggtcgaacc atacgacgct aaagtactga tgccagcagg aggtgccgta ccatggccag
     1921 aattcctagc actgagtgag agcgccacgt tagtgtacaa cgaaagagag tttgtgaacc
     1981 gcaaactata ccacattgcc atgcatggcc ccgccaagaa tacagaagag gagcagtaca
     2041 aggttacaaa ggcagagctt gcagaaacag agtacgtgtt tgacgtggac aagaagcgtt
     2101 gcgttaagaa ggaagaagcc tcaggtctgg tcctctcggg agaactgacc aaccctccct
     2161 atcatgagct agctctggag ggactgaaga cccgacctgc ggtcccgtac aaggtcgaaa
     2221 caataggagt gataggcaca ccggggtcgg gcaagtcagc tattatcaag tcaactgtca
     2281 cggcacgaga tcttgttacc agcggaaaga aagaaaattg tcgcgaaatt gaggccgacg
     2341 tgctaagact gaggggtatg cagattacgt cgaagacagt agattcggtt atgctcaacg
     2401 gatgccacaa agccgtagaa gtgctgtacg ttgacgaagc gttcgcgtgc cacgcaggag
     2461 cactacttgc cttgattgct atcgtcaggc cccgcaagaa ggtagtacta tgcggagacc
     2521 ccatgcaatg cggattcttc aacatgatgc aactaaaggt acatttcaat caccctgaaa
     2581 aagacatatg caccaagaca ttctacaagt atatctcccg gcgttgcaca cagccagtta
     2641 cagctattgt atcgacactg cattacgatg gaaagatgaa aaccacgaac ccgtgcaaga
     2701 agaacattga aatcgatatt acaggggcca caaagccgaa gccaggggat atcatcctga
     2761 catgtttccg cgggtgggtt aagcaattgc aaatcgacta tcccggacat gaagtaatga
     2821 cagccgcggc ctcacaaggg ctaaccagaa aaggagtgta tgccgtccgg caaaaagtca
     2881 atgaaaaccc actgtacgcg atcacatcag agcatgtgaa cgtgttgctc acccgcactg
     2941 aggacaggct agtgtggaaa accttgcagg gcgacccatg gattaagcag cccactaaca
     3001 tacctaaagg aaactttcag gctactatag aggactggga agctgaacac aagggaataa
     3061 ttgctgcaat aaacagcccc actccccgtg ccaatccgtt cagctgcaag accaacgttt
     3121 gctgggcgaa agcattggaa ccgatactag ccacggccgg tatcgtactt accggttgcc
     3181 agtggagcga actgttccca cagtttgcgg atgacaaacc acattcggcc atttacgcct
     3241 tagacgtaat ttgcattaag tttttcggca tggacttgac aagcggactg ttttctaaac
     3301 agagcatccc actaacgtac catcccgccg attcagcgag gccggtagct cattgggaca
     3361 acagcccagg aacccgcaag tatgggtacg atcacgccat tgccgccgaa ctctcccgta
     3421 gatttccggt gttccagcta gctgggaagg gcacacaact tgatttgcag acggggagaa
     3481 ccagagttat ctctgcacag cataacctgg tcccggtgaa ccgcaatctt cctcacgcct
     3541 tagtccccga gtacaaggag aagcaacccg gcccggtcaa aaaattcttg aaccagttca
     3601 aacaccactc agtacttgtg gtatcagagg aaaaaattga agctccccgt aagagaatcg
     3661 aatggatcgc cccgattggc atagccggtg cagataagaa ctacaacctg gctttcgggt
     3721 ttccgccgca ggcacggtac gacctggtgt tcatcaacat tggaactaaa tacagaaacc
     3781 accactttca gcagtgcgaa gaccatgcgg cgaccttaaa aaccctttcg cgttcggccc
     3841 tgaattgcct taacccagga ggcaccctcg tggtgaagtc ctatggctac gccgaccgca
     3901 acagtgagga cgtagtcacc gctcttgcca gaaagtttgt cagggtgtct gcagcgagac
     3961 cagattgtgt ctcaagcaat acagaaatgt acctgatttt ccgacaacta gacaacagcc
     4021 gtacacggca attcaccccg caccatctga attgcgtgat ttcgtccgtg tatgagggta
     4081 caagagatgg agttggagcc gcgccgtcat accgcaccaa aagggagaat attgctgact
     4141 gtcaagagga agcagttgtc aacgcagcca atccgctggg tagaccaggc gaaggagtct
     4201 gccgtgccat ctataaacgt tggccgacca gttttaccga ttcagccacg gagacaggca
     4261 ccgcaagaat gactgtgtgc ctaggaaaga aagtgatcca cgcggtcggc cctgatttcc
     4321 ggaagcaccc agaagcagaa gccttgaaat tgctacaaaa cgcctaccat gcagtggcag
     4381 acttagtaaa tgaacataac atcaagtctg tcgccattcc actgctatct acaggcattt
     4441 acgcagccgg aaaagaccgc cttgaagtat cacttaactg cttgacaacc gcgctagaca
     4501 gaactgacgc ggacgtaacc atctattgcc tggataagaa gtggaaggaa agaatcgacg
     4561 cggcactcca acttaaggag tctgtaacag agctgaagga tgaagatatg gagatcgacg
     4621 atgagttagt atggattcat ccagacagtt gcttgaaggg aagaaaggga ttcagtacta
     4681 caaaaggaaa attgtattcg tacttcgaag gcaccaaatt ccatcaagca gcaaaagaca
     4741 tggcggagat aaaggtcctg ttccctaatg accaggaaag taatgaacaa ctgtgtgcct
     4801 acatattggg tgagaccatg gaagcaatcc gcgaaaagtg cccggtcgac cataacccgt
     4861 cgtctagccc gcccaaaacg ttgccgtgcc tttgcatgta tgccatgacg ccagaaaggg
     4921 tccacagact tagaagcaat aacgtcaaag aagttacagt atgctcctcc accccccttc
     4981 ctaagcacaa aattaagaat gttcagaagg ttcagtgcac gaaagtagtc ctgtttaatc
     5041 cgcacactcc cgcattcgtt cccgcccgta agtacataga agtgccagaa cagcctaccg
     5101 ctcctcctgc acaggccgag gaggcccccg aagttgtagc gacaccgtca ccatctacag
     5161 ctgataacac ctcgcttgat gtcacagaca tctcactgga tatggatgac agtagcgaag
     5221 gctcactttt ttcgagcttt agcggatcgg acaactctat tactagtatg gacagttggt
     5281 cgtcaggacc tagttcacta gagatagtag accgaaggca ggtggtggtg gctgacgttc
     5341 atgccgtcca agagcctgcc cctattccac cgccaaggct aaagaagatg gcccgcctgg
     5401 cagcggcaag aaaagagccc actccaccgg caagcaatag ctctgagtcc ctccacctct
     5461 cttttggtgg ggtatccatg tccctcggat caattttcga cggagagacg gcccgccagg
     5521 cagcggtaca acccctggca acaggcccca cggatgtgcc tatgtctttc ggatcgtttt
     5581 ccgacggaga gattgatgag ctgagccgca gagtaactga gtccgaaccc gtcctgtttg
     5641 gatcatttga accgggcgaa gtgaactcaa ttatatcgtc ccgatcagcc gtatcttttc
     5701 cactacgcaa gcagagacgt agacgcagga gcaggaggac tgaatactga ctaaccgggg
     5761 taggtgggta catattttcg acggacacag gccctgggca cttgcaaaag aagtccgttc
     5821 tgcagaacca gcttacagaa ccgaccttgg agcgcaatgt cctggaaaga attcatgccc
     5881 cggtgctcga cacgtcgaaa gaggaacaac tcaaactcag gtaccagatg atgcccaccg
     5941 aagccaacaa aagtaggtac cagtctcgta aagtagaaaa tcagaaagcc ataaccactg
     6001 agcgactact gtcaggacta cgactgtata actctgccac agatcagcca gaatgctata
     6061 agatcaccta tccgaaacca ttgtactcca gtagcgtacc ggcgaactac tccgatccac
     6121 agttcgctgt agctgtctgt aacaactatc tgcatgagaa ctatccgaca gtagcatctt
     6181 atcagattac tgacgagtac gatgcttact tggatatggt agacgggaca gtcgcctgcc
     6241 tggatactgc aaccttctgc cccgctaagc ttagaagtta cccgaaaaaa catgagtata
     6301 gagccccgaa tatccgcagt gcggttccat cagcgatgca gaacacgcta caaaatgtgc
     6361 tcattgccgc aactaaaaga aattgcaacg tcacgcagat gcgtgaactg ccaacactgg
     6421 actcagcgac attcaatgtc gaatgctttc gaaaatatgc atgtaatgac gagtattggg
     6481 aggagttcgc tcggaagcca attaggatta ccactgagtt tgtcaccgca tatgtagcta
     6541 gactgaaagg ccctaaggcc gccgcactat ttgcaaagac gtataatttg gtcccattgc
     6601 aagaagtgcc tatggataga ttcgtcatgg acatgaaaag agacgtgaaa gttacaccag
     6661 gcacgaaaca cacagaagaa agaccgaaag tacaagtgat acaagccgca gaacccctgg
     6721 cgactgctta cttatgcggg attcaccggg aattagtgcg taggcttacg gccgtcttgc
     6781 ttccaaacat tcacacgctt tttgacatgt cggcggagga ttttgatgca atcatagcag
     6841 aacacttcaa gcaaggcgac ccggtactgg agacggatat cgcatcattc gacaaaagcc
     6901 aagacgacgc tatggcgtta accggtctga tgatcttgga ggacctgggt gtggatcaac
     6961 cactactcga cttgatcgag tgcgcctttg gagaaatatc atccacccat ctacctacgg
     7021 gtactcgttt taaattcggg gcgatgatga aatccggaat gttcctcaca ctttttgtca
     7081 acacagtttt gaatgtcgtt atcgccagca gagtactaga agagcggctt aaaacgtcca
     7141 gatgtgcagc gttcattggc gacgacaaca tcatacatgg agtagtatct gacaaagaaa
     7201 tggctgagag gtgcgccacc tggctcaaca tggaggttaa gatcatcgac gcagtcatcg
     7261 gtgagagacc accttacttc tgcggcggat ttatcttgca agattcggtt acttccacag
     7321 cgtgccgcgt ggcggatccc ctgaaaaggc tgtttaagtt gggtaaaccg ctcccagccg
     7381 acgacgagca agacgaagac agaagacgcg ctctgctaga tgaaacaaag gcgtggttta
     7441 gagtaggtat aacaggcact ttagcagtgg ccgtgacgac ccggtatgag gtagacaata
     7501 ttacacctgt cctactggca ttgagaactt ttgcccagag caaaagagca ttccaagcca
     7561 tcagagggga aattaaagca tctctacggt ggtcctaaat agtcagcata gtacatttca
     7621 tctgactaat accacaacac caccacctct agagccacca tgccaccttc tactagtctt
     7681 ttgttgcttg ctgcacttct ccccttcgct cttcccgctt cagattggaa gacaggagaa
     7741 gtcaccgctt cccgcgacca catggtgctg cacgaatatg tgaacgctgc tggcatcaca
     7801 ggcggtggcg gatctggcgg cggtggtagc gtcgacgaac aaaaactcat ctcagaagag
     7861 gatctacaat tcatggacaa agactgcgaa atgaagcgca ccaccctgga tagccctctg
     7921 ggcaagctgg aactgtctgg gtgcgaacag ggcctgcacc gtatcatctt cctgggcaaa
     7981 ggaacatctg ccgccgacgc cgtggaagtg cctgccccag ccgccgtgct gggcggacca
     8041 gagccactga tccaggccac cgcctggctc aacgcctact ttcaccagcc tgaggccatc
     8101 gaggagttcc ctgtgccagc cctgcaccac ccagtgttcc agcaggagag ctttacccgc
     8161 caggtgctgt ggaaactgct gaaagtggtg aagttcggag aggtcatcag cgagagccac
     8221 ctggccgccc tggtgggcaa tcccgccgcc accgccgccg tgaacaccgc cctggacgga
     8281 aatcccgtgc ccattctgat cccctgccac cgggtggtgc agggcgacag cgacgtgggg
     8341 ccctacctgg gcgggctcgc cgtgaaagag tggctgctgg cccacgaggg ccacagactg
     8401 ggcaagcctg ggctgggtgg cggtggcgga tctgtcgact ttcagaaagc tagctctatt
     8461 gtttataaga aggaggggga acaggtggag ttcagtttcc cattggcctt cacagtcgag
     8521 aagctcacag gatcaggaga gttgtggtgg caggccgaaa gagcctcctc cagcaagagt
     8581 tggatcactt ttgatctgaa aaacaaagaa gtgagcgtga agagagtcac acaggaccct
     8641 aagctgcaga tgggcaagaa gttgcctttg cacttgacac tcccccaagc cctgccccag
     8701 tatgctggct ccgggaacct caccctcgcc ttggaggcta agaccggaaa gttgcatcag
     8761 gaggtcaacc tcgtggtgat gagggcaaca cagctgcaga agaacctgac ctgcgaagtg
     8821 tggggtccca cctcacctaa actgatgctg tctctgaagc tggagaataa ggaggctaaa
     8881 gtcagtaaaa gagagaaggc tgtgtgggtc cttaacccgg aggcagggat gtggcagtgt
     8941 cttcttagcg actctggaca ggtgctgctg gagagcaaca tcaaggtgct tcctacttgg
     9001 tcaacaccag tacagccgat ggccctgatc gtactcgggg gagtcgccgg cctgcttctg
     9061 tttatcggcc tgggcatctt cttctgcgta cgctacaagt atcgcaacag ggatgaggga
     9121 tctagcgctc caccgctcga cggagccgga gctggcgctg gagctggagc cggagctggc
     9181 gggctagcca ccatggacgc acaaacacga cgacgtgagc gtcgcgctga gaaacaagct
     9241 caatggaaag ctgcaaaccc accgctcgac ggagccggag ctggcgctgg agctggagcc
     9301 ggagctggcg gtctagccac catggacgca caaacacgac gacgtgagcg tcgcgctgag
     9361 aaacaagctc aatggaaagc tgcaaaccca ccgctcgacg gagccggagc tggcgctgga
     9421 gctggagccg gagctggcgg tctagccacc atggacgcac aaacacgacg acgtgagcgt
     9481 cgcgctgaga aacaagctca atggaaagct gcaaacccac cgctcgacgg agccggagct
     9541 ggcgctggag ctggagccgg agctggcggt ctagccacca tggacgcaca aacacgacga
     9601 cgtgagcgtc gcgctgagaa acaagctcaa tggaaagctg caaacccacc gctcgagcca
     9661 ccgctcgacg gagccggagc tggcgctgga gctggagccg gagctggcgg tctagccacc
     9721 ccaccgagcg cttaccacgt tgacgaatct aggaactata tcagtaacag tgctcagagt
     9781 aatggcgccg tggttaaaga gaaacagccc tccagcgcca aatcagcaaa caaaaacaag
     9841 aaaaacaagg ataaagagta ctacgtatag gcggcgcatg caggccttaa agcatctcta
     9901 cggtggtcct aaatagtcag catagtacat ttcatctgac taataccaca acaccaccac
     9961 ctctagtaga ggcgcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg
    10021 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
    10081 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
    10141 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
    10201 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
    10261 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
    10321 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
    10381 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
    10441 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
    10501 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
    10561 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
    10621 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
    10681 atggacgagc tgtacaagta aacgcgtgag aacttcagcg gccgctacct aattgccgtc
    10741 gtgaggtacg accaccgcta gctgtacagc cctgaaaaag ggctcgagcc ctgaaaaagg
    10801 gcaattgccc tgaaaaaggg cgtcgacgcc ctgaaaaagg gcggaattca tgtccaattt
    10861 actgactgta caccaaaatt tgcctgcggg cccaatgatc cgaccagcaa aactcgatgt
    10921 acttccgagg aactgatgtg cataatgcat caggctggta cattagatcc ccgcttaccg
    10981 cgggcaatat agcaacacta aaaactcgat gtacttccga ggaagcgcag tgcataatgt
    11041 tgcgcagtgt tgccacataa ccactatatt aaccatttat ttagcggacg ccaaaaactc
    11101 aatgtatttt tgaggaagcg tggtgcataa tgccacgcag gttttgcata attttttttt
    11161 ttttttttat taatcaacaa aattttgttt ttaacatttc aaaaaaaaaa aaaaaaaaaa
    11221 aaaaaaaaaa aaaaggggaa ttcctcgata attaagcggc ctagattatg taggttgtga
    11281 gatgcgggag gttctcgatc ttcccgtggg acgtcaaggc cgcttaatta atcgagggga
    11341 attaattctt gaagacgaaa gggccaggtg gcacttttcg gggaaatgtg cgcggaaccc
    11401 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
    11461 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
    11521 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
    11581 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
    11641 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
    11701 cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac
    11761 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
    11821 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
    11881 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
    11941 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
    12001 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
    12061 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
    12121 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
    12181 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
    12241 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
    12301 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
    12361 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
    12421 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
    12481 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
    12541 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
    12601 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
    12661 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
    12721 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
    12781 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
    12841 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
    12901 gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
    12961 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
    13021 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
    13081 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg agctcgtatg
    13141 gacatattgt cgttagaacg cggctacaat taatacataa ccttatgtat catacacata
    13201 cgatttaggg gacactatag
//
