LOCUS       Exported               10687 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Expresses human NEUROGENIN2 (hNGN2), eGFP and puromycin resistance 
            gene under control of TetON promoter. This 3rd generation lentiviral
            vector is used to generate NGN2-iNs from hiPSCs and hiPSC-NPCs..
ACCESSION   .
VERSION     .
KEYWORDS    pLV-TetO-hNGN2-eGFP-Puro
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10687)
  AUTHORS   Ho SM, Hartley BJ, Tcw J, Beaumont M, Stafford K, Slesinger PA, 
            Brennand KJ
  TITLE     Rapid Ngn2-induction of excitatory neurons from hiPSC-derived neural
            progenitor cells.
  JOURNAL   Methods. 2015 Nov 25. pii: S1046-2023(15)30159-6. doi: 
            10.1016/j.ymeth.2015.11.019.
  PUBMED    26626326
REFERENCE   2  (bases 1 to 10687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10687
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             844..900
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             913..1623
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     CDS             913..1623
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     primer_bind     complement(952..973)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1213..1232)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1560..1581
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1633..1686
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1699..2292
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="EYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERVT
                     ELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAAQ
                     QQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSAP
                     RNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     2189..2209
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2331..2919
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2384..2404)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2802..2813)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2922..2938)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2923..2939)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3444..3624
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3650..3667)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3656..3880
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3926..4354
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4013..4032)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4223..4244
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4363..4383)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4368..4697
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4548..4683
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4610..4629
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4745..4792
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4811..5185
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5315..5436
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5352..5371)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5406..5425
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5485..5501)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5485..5501)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5498..5520)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5509..5525
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5533..5563)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5578..5599
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5716..5733)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5887..6475)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5967..5986)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6646..7506)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7269..7288
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7507..7611)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7686..7705)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7877..8256
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8258..8456
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8411..8431
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8474..8654
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8701..8826
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9319..9552
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9737..9781
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10079..10196
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    10253..10523
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        10556..10594
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10591..10615
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gaattcgcca ccatgttcgt caaatccgag accttggagt tgaaggagga agaggacgtg
       61 ttagtgctgc tcggatcggc ctcccccgcc ttggcggccc tgaccccgct gtcatccagc
      121 gccgacgaag aagaggagga ggagccgggc gcgtcaggcg gggcgcgtcg gcagcgcggg
      181 gctgaggccg ggcagggggc gcggggcggc gtggctgcgg gtgcggaggg ctgccggccc
      241 gcacggctgc tgggtctggt acacgattgc aaacggcgcc cttcccgggc gcgggccgtc
      301 tcccgaggcg ccaagacggc cgagacggtg cagcgcatca agaagacccg tagactgaag
      361 gccaacaacc gcgagcgaaa ccgcatgcac aacctcaacg cggcactgga cgcgctgcgc
      421 gaggtgctcc ccacgttccc cgaggacgcc aagctcacca agatcgagac cctgcgcttc
      481 gcccacaact acatctgggc actcaccgag accctgcgcc tggcggatca ctgcgggggc
      541 ggcggcgggg gcctgccggg ggcgctcttc tccgaggcag tgttgctgag cccgggagga
      601 gccagcgccg ccctgagcag cagcggagac agcccctcgc ccgcctccac gtggagttgc
      661 accaacagcc ccgcgccgtc ctcctccgtg tcctccaatt ccacctcccc ctacagctgc
      721 actttatcgc ccgccagccc ggccgggtca gacatggact attggcagcc cccacctccc
      781 gacaagcacc gctatgcacc tcacctcccc atagccaggg attgtatcga attcggatct
      841 ggcgccacga atttctcgct actcaagcag gccggtgatg tcgaggaaaa ccctggtcct
      901 atggtgtcta gaagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
      961 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     1021 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     1081 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     1141 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     1201 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     1261 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     1321 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     1381 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     1441 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     1501 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     1561 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     1621 aagggttcag gggagggaag aggttccctg ctcacatgcg gagacgtaga agagaatccc
     1681 ggacccatga cctctagaga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     1741 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     1801 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     1861 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     1921 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     1981 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     2041 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     2101 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     2161 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     2221 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     2281 cccggtgcct gattaattaa gctagctaat tcgatatcaa gcttatcgat aatcaacctc
     2341 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     2401 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     2461 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     2521 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     2581 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     2641 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     2701 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     2761 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     2821 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     2881 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgag
     2941 acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg
     3001 cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt
     3061 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     3121 gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc
     3181 acacacaagg ctacttccct gattggcaga actacacacc agggccaggg atcagatatc
     3241 cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag gtagaagaag
     3301 ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg atggatgacc
     3361 cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat cacatggccc
     3421 gagagctgca tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc
     3481 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     3541 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     3601 agtcagtgtg gaaaatctct agcagggccc gtttaaaccc gctgatcagc ctcgactgtg
     3661 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     3721 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     3781 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     3841 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     3901 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
     3961 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     4021 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     4081 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     4141 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     4201 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     4261 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     4321 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
     4381 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     4441 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     4501 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     4561 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     4621 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     4681 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc
     4741 acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag
     4801 gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc
     4861 cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga
     4921 cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt
     4981 ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga
     5041 gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat
     5101 cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca
     5161 cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt
     5221 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     5281 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
     5341 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     5401 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
     5461 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     5521 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     5581 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     5641 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     5701 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     5761 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     5821 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     5881 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     5941 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     6001 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     6061 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     6121 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     6181 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     6241 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     6301 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     6361 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     6421 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     6481 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     6541 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     6601 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     6661 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     6721 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     6781 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     6841 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     6901 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     6961 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     7021 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     7081 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     7141 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     7201 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     7261 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     7321 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     7381 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     7441 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     7501 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     7561 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     7621 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact
     7681 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg
     7741 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc
     7801 gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg
     7861 gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
     7921 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
     7981 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     8041 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     8101 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     8161 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     8221 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
     8281 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
     8341 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
     8401 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg
     8461 ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     8521 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     8581 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     8641 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     8701 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     8761 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     8821 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     8881 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     8941 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     9001 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     9061 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     9121 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
     9181 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     9241 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     9301 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     9361 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     9421 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     9481 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     9541 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     9601 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     9661 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     9721 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     9781 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     9841 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     9901 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
     9961 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
    10021 gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt
    10081 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
    10141 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
    10201 tttattacag ggacagcaga gatccagttt ggttagatct cgagtttacc actccctatc
    10261 agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga
    10321 aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc
    10381 cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa
    10441 aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta
    10501 ccactcccta tcagtgatag agaaaagtga aagtcgagct cggtacccgg gtcgaggtag
    10561 gcgtgtacgg tgggaggcct atataagcag agctcgttta gtgaaccgtc agatcgcctg
    10621 gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccg
    10681 cggcccc
//
