LOCUS       Exported                9901 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Expresses human NEUROGENIN2 (hNGN2) and puromycin resistance gene 
            under control of TetON promoter. This lentiviral vector is used to 
            generate NGN2-iNs (induced neurons) from hiPSCs and hiPSC-NPCs..
ACCESSION   .
VERSION     .
KEYWORDS    pLV-TetO-hNGN2-Puro
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9901)
  AUTHORS   Ho SM, Hartley BJ, Tcw J, Beaumont M, Stafford K, Slesinger PA, 
            Brennand KJ
  TITLE     Rapid Ngn2-induction of excitatory neurons from hiPSC-derived neural
            progenitor cells.
  JOURNAL   Methods. 2015 Nov 25. pii: S1046-2023(15)30159-6. doi: 
            10.1016/j.ymeth.2015.11.019.
  PUBMED    26626326
REFERENCE   2  (bases 1 to 9901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9901
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             844..900
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             913..1506
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="EYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERVT
                     ELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAAQ
                     QQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSAP
                     RNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     1403..1423
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    1545..2133
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1598..1618)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2016..2027)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2136..2152)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2137..2153)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2658..2838
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2864..2881)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2870..3094
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3140..3568
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3227..3246)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3437..3458
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3577..3597)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3582..3911
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3762..3897
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3824..3843
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        3959..4006
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4025..4399
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4529..4650
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4566..4585)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4620..4639
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4699..4715)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4699..4715)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4712..4734)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4723..4739
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4747..4777)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4792..4813
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4930..4947)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5101..5689)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5181..5200)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5860..6720)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6483..6502
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6721..6825)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6900..6919)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7091..7470
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7472..7670
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7625..7645
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             7688..7868
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7915..8040
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8533..8766
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8951..8995
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9293..9410
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    9467..9737
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        9770..9808
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9805..9829
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gaattcgcca ccatgttcgt caaatccgag accttggagt tgaaggagga agaggacgtg
       61 ttagtgctgc tcggatcggc ctcccccgcc ttggcggccc tgaccccgct gtcatccagc
      121 gccgacgaag aagaggagga ggagccgggc gcgtcaggcg gggcgcgtcg gcagcgcggg
      181 gctgaggccg ggcagggggc gcggggcggc gtggctgcgg gtgcggaggg ctgccggccc
      241 gcacggctgc tgggtctggt acacgattgc aaacggcgcc cttcccgggc gcgggccgtc
      301 tcccgaggcg ccaagacggc cgagacggtg cagcgcatca agaagacccg tagactgaag
      361 gccaacaacc gcgagcgaaa ccgcatgcac aacctcaacg cggcactgga cgcgctgcgc
      421 gaggtgctcc ccacgttccc cgaggacgcc aagctcacca agatcgagac cctgcgcttc
      481 gcccacaact acatctgggc actcaccgag accctgcgcc tggcggatca ctgcgggggc
      541 ggcggcgggg gcctgccggg ggcgctcttc tccgaggcag tgttgctgag cccgggagga
      601 gccagcgccg ccctgagcag cagcggagac agcccctcgc ccgcctccac gtggagttgc
      661 accaacagcc ccgcgccgtc ctcctccgtg tcctccaatt ccacctcccc ctacagctgc
      721 actttatcgc ccgccagccc ggccgggtca gacatggact attggcagcc cccacctccc
      781 gacaagcacc gctatgcacc tcacctcccc atagccaggg attgtatcga attcggatct
      841 ggcgccacga atttctcgct actcaagcag gccggtgatg tcgaggaaaa ccctggtcct
      901 atggtgtcta gagagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
      961 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     1021 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     1081 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
     1141 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     1201 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     1261 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     1321 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     1381 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     1441 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     1501 gcctgattaa ttaagctagc taattcgata tcaagcttat cgataatcaa cctctggatt
     1561 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     1621 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     1681 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     1741 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     1801 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     1861 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     1921 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
     1981 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     2041 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     2101 cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgagacctag
     2161 aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc
     2221 tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac
     2281 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     2341 aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac
     2401 aaggctactt ccctgattgg cagaactaca caccagggcc agggatcaga tatccactga
     2461 cctttggatg gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg
     2521 aaggagagaa cacccgcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
     2581 gagaagtatt agagtggagg tttgacagcc gcctagcatt tcatcacatg gcccgagagc
     2641 tgcatccgga ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     2701 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     2761 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     2821 tgtggaaaat ctctagcagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
     2881 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     2941 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     3001 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     3061 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     3121 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     3181 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     3241 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     3301 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     3361 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     3421 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     3481 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     3541 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
     3601 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     3661 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     3721 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     3781 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     3841 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     3901 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt
     3961 tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta
     4021 aaccatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc
     4081 ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc
     4141 cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc
     4201 ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc
     4261 ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga
     4321 gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt
     4381 ggccgaggag caggactgac acgtgctacg agatttcgat tccaccgccg ccttctatga
     4441 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     4501 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
     4561 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     4621 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
     4681 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     4741 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     4801 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     4861 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     4921 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     4981 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     5041 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     5101 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     5161 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     5221 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     5281 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     5341 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     5401 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     5461 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     5521 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     5581 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     5641 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     5701 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     5761 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     5821 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     5881 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     5941 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     6001 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     6061 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     6121 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     6181 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     6241 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     6301 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     6361 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     6421 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     6481 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     6541 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     6601 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     6661 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     6721 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     6781 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     6841 agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt
     6901 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag
     6961 gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat
     7021 tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga
     7081 tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
     7141 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     7201 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     7261 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     7321 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     7381 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     7441 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     7501 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     7561 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     7621 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc
     7681 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     7741 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     7801 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     7861 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
     7921 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     7981 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     8041 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     8101 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     8161 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     8221 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     8281 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     8341 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
     8401 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     8461 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     8521 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     8581 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     8641 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     8701 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     8761 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     8821 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     8881 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     8941 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     9001 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     9061 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     9121 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
     9181 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
     9241 atcggcactg cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa
     9301 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     9361 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     9421 acagggacag cagagatcca gtttggttag atctcgagtt taccactccc tatcagtgat
     9481 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
     9541 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
     9601 agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga
     9661 aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc
     9721 cctatcagtg atagagaaaa gtgaaagtcg agctcggtac ccgggtcgag gtaggcgtgt
     9781 acggtgggag gcctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
     9841 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccgcggccc
     9901 c
//
