LOCUS Exported 9500 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expresses RpBphP1-mCherry-TetR under CMVd1 promoter. ACCESSION . VERSION . KEYWORDS pT2/SVNeo-103 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9500) AUTHORS Kaberniuk AA, Shemetov AA, Verkhusha VV TITLE A bacterial phytochrome-based optogenetic system controllable with near-infrared light. JOURNAL Nat Methods. 2016 May 9. doi: 10.1038/nmeth.3864. PUBMED 27159085 REFERENCE 2 (bases 1 to 9500) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9500 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 34..56 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 48..65 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 49..65 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 75..93 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" promoter 482..581 /label=CMVd1 promoter /note="truncated human cytomegalovirus (CMV) immediate early promoter that yields slightly reduced expression relative to the full-length promoter" primer_bind 532..552 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" promoter 613..631 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" promoter 637..655 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind 637..654 /label=SP6 /note="SP6 promoter, forward primer" CDS 2916..3623 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /label=mCherry /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" primer_bind complement(3061..3079) /label=mCherry-R /note="mCherry, reverse primer" primer_bind 3329..3348 /label=mCherry-F /note="mCherry, forward primer" CDS 3696..4319 /codon_start=1 /gene="tetR from transposon Tn10" /product="tetracycline repressor TetR" /label=TetR /note="TetR binds to the tetracycline operator tetO to inhibit transcription. This inhibition can be relieved by adding tetracycline or doxycycline." /translation="MTRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS" primer_bind complement(3769..3788) /label=Tet-R /note="Tetracycline resistance gene, reverse primer" polyA_signal 4343..4464 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(4835..4855) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 4840..5169 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 5020..5155 /label=SV40 ori /note="SV40 origin of replication" primer_bind 5082..5101 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 5236..6030 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(5290..5309) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 5900..5919 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 6204..6338 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(6761..6781) /label=T3 /note="T3 promoter, forward primer" promoter complement(6761..6779) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(6800..6816) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6800..6816) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(6813..6835) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 6824..6840 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(6848..6878) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6893..6914 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(7031..7048) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(7202..7790) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(7282..7301) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(7961..8821) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 8584..8603 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(8822..8926) /gene="bla" /label=AmpR promoter rep_origin 8953..9408 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(9040..9059) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 9250..9271 /label=F1ori-F /note="F1 origin, forward primer" ORIGIN 1 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg 61 ccagtgagcg cgcgtaatac gactcactat agggcgaatt ggagctcgga tccctataca 121 gttgaagtcg gaagtttaca tacacttaag ttggagtcat taaaactcgt ttttcaacta 181 ctccacaaat ttcttgttaa caaacaatag ttttggcaag tcagttagga catctacttt 241 gtgcatgaca caagtcattt ttccaacaat tgtttacaga cagattattt cacttataat 301 tcactgtatc acaattccag tgggtcagaa gtttacatac actaagttga ctgtgccttt 361 aaacagcttg gaaaattcca gaaaatgatg tcatggcttt agaagcttcc cacaataagt 421 tgggtgaatt ttggctcatt cctcctttct ataggattga ggtcagagct ttgtgatggc 481 tccaaaatca acgggacttt ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg 541 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tggtttagtg aaccgtcaga 601 tcactagaag cttaatacga ctcactatag ggtacgattt aggtgacact atagaataag 661 gagcgatcgc catggctagc gtggcaggtc atgcctctgg cagccccgca ttcgggaccg 721 ccgatctttc gaattgcgaa cgtgaagaga tccacctcgc cggctcgatc cagccgcatg 781 gcgcgcttct ggtcgtcagc gagccggatc atcgcatcat ccaggccagc gccaacgccg 841 cggaatttct gaatctcgga agcgtgctcg gcgttccgct cgccgagatc gacggcgatc 901 tgttgatcaa gatcctgccg catctcgatc ccaccgccga aggcatgccg gtcgcggtgc 961 gctgccggat cggcaatccc tccacggagt acgacggtct gatgcatcgg cctccggaag 1021 gcgggctgat catcgagctc gaacgtgccg gcccgccgat cgatctgtcc ggcacgctgg 1081 cgccggcgct ggagcggatc cgcacggcgg gctcgctgcg cgcgctgtgc gatgacaccg 1141 cgctgctgtt tcagcagtgc accggctacg accgggtgat ggtgtatcgc ttcgacgagc 1201 agggccacgg cgaagtgttc tccgagcgcc acgtgcccgg gctcgaatcc tatttcggca 1261 accgctatcc gtcgtcggac attccgcaga tggcgcggcg gctgtacgag cggcagcgcg 1321 tccgcgtgct ggtcgacgtc agctatcagc cggtgccgct ggagccgcgg ctgtcgccgc 1381 tgaccgggcg cgatctcgac atgtcgggct gcttcctgcg ctcgatgtcg ccgatccatc 1441 tgcagtacct gaagaacatg ggcgtgcgcg ccaccctggt ggtgtcgctg gtggtcggcg 1501 gcaagctgtg gggcctggtt gcctgtcatc attatctgcc gcgcttcatg catttcgagc 1561 tgcgggcgat ctgcgaactg ctcgccgaag cgatcgcgac gcggatcacc gcgcttgaga 1621 gcttcgcgca gagccagtcg gagctgttcg tgcagcggct cgaacagcgc atgatcgaag 1681 cgattacccg tgaaggcgat tggcgcgcag cgattttcga caccagccaa tcgatcctgc 1741 agccgctgca cgccgccggt tgcgcgctgg tgtacgaaga ccagatcagg accatcggcg 1801 acgtgccttc cacgcaggat gtgcgcgaga tcgccgggtg gctcgatcgc cagccgcgcg 1861 cggcggtgac ctcgaccgcg tcgctcggtc tcgacgtgcc ggagctcgcg catctgacgc 1921 ggatggcgag cggcgtggtc gcggcgccga tttcggatca tcgcggcgag tttctgatgt 1981 ggttccgccc cgagcgcgtc cacaccgtta cctggggcgg cgatccgaag aagccgttca 2041 cgatgggcga tacaccggcg gatctgtcgc cgcggcgctc cttcgccaaa tggcatcagg 2101 ttgtcgaagg cacgtccgat ccgtggacgg ccgccgatct cgccgcggct cgcaccatcg 2161 gtcagaccgt cgccgacatc gtgctgcaat tccgcgcggt gcggacactg atcgcccgcg 2221 aacagtacga acagttttcg tcccaggtgc acgcttcgat gcagccggtg ctgatcaccg 2281 acgccgaagg ccgcatcctg ctgatgaacg actcgttccg cgacatgttg ccggcgggtt 2341 cgccatccgc cgtccatctc gacgatctcg ccgggttctt cgtcgaatcg aacgatttcc 2401 tgcgcaacgt cgccgaactg atcgatcacg gccgcgggtg gcgcggcgaa gttctgctgc 2461 gcggcgcagg caaccgcccg ttgccgctgg cagtgcgcgc cgatccggtg acgcgcacgg 2521 aggaccagtc gctcggcttc gtgctgatct tcagcgacgc taccgatcgt cgcaccgcag 2581 atgccgcacg cacgcgtttc caggaaggca ttcttgccag cgcacgtccc ggcgtgcggc 2641 tcgactccaa gtccgacctg ttgcacgaga agctgctgtc cgcgctggtc gagaacgcgc 2701 agcttgccgc attggaaatc acttacggcg tcgagaccgg acgcatcgcc gagctgctcg 2761 aaggcgtccg ccagtcgatg ctgcgcaccg ccgaagtgct cggccatctg gtgcagcacg 2821 cggcgcgcac ggccggcagc gacagctcga gcaatggctc gcagaacaag aaggaattcg 2881 atagtgctgg tagtgctggt agtgctggta ctagtatggt gagcaagggc gaggaggata 2941 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg 3001 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg 3061 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc 3121 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga 3181 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg 3241 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc 3301 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg 3361 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga 3421 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca 3481 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc 3541 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg 3601 gcggcatgga cgagctgtac aagagtgctg gtgggtccgc cggcgggagt gctggcggta 3661 gtgccggcgg tagttctaga agtgctggtg ggtccatgac tagattagat aaaagtaaag 3721 tgattaacag cgcattagag ctgcttaatg aggtcggaat cgaaggttta acaacccgta 3781 aactcgccca gaagctaggt gtagagcagc ctacattgta ttggcatgta aaaaataagc 3841 gggctttgct cgacgcctta gccattgaga tgttagatag gcaccatact cacttttgcc 3901 ctttagaagg ggaaagctgg caagattttt tacgtaataa cgctaaaagt tttagatgtg 3961 ctttactaag tcatcgcgat ggagcaaaag tacatttagg tacacggcct acagaaaaac 4021 agtatgaaac tctcgaaaat caattagcct ttttatgcca acaaggtttt tcactagaga 4081 atgcattata tgcactcagc gctgtggggc attttacttt aggttgcgta ttggaagatc 4141 aagagcatca agtcgctaaa gaagaaaggg aaacacctac tactgatagt atgccgccat 4201 tattacgaca agctatcgaa ttatttgatc accaaggtgc agagccagcc ttcttattcg 4261 gccttgaatt gatcatatgc ggattagaaa aacaacttaa atgtgaaagt gggtcctaag 4321 cggccgcttc gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga 4381 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc 4441 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt 4501 cagggggaga tgtgggaggt ttttttaagc aagtaaaacc tctacaaatg tggtaaaatc 4561 gaattctaat ggatcctctt tgcgcttgcg ttttcccttg tccagatagc ccagtagctg 4621 acattcatcc ggggtcagca ccgtttctgc ggactggctt tctacgtgtt ccgcttcctt 4681 tagcagccct tgcgccctga gtgcttgcgg cagcgtgagc ttcaaaagaa ttgccagctg 4741 gggcgccctc tggtaaggtt gggaagccct gcaaagtaaa ctggatggct ttcttgccgc 4801 caaggatctg atggcgcagg ggatcaaatt ctgtggaatg tgtgtcagtt agggtgtgga 4861 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca 4921 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc 4981 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc 5041 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag 5101 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc 5161 ttttgcaaaa agctcccggg agcttggata tccattttcg gatctgatca agagacagga 5221 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg 5281 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc 5341 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt 5401 gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt 5461 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc 5521 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc 5581 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac 5641 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag 5701 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag 5761 gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat 5821 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg 5881 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa 5941 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc 6001 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc 6061 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt 6121 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca 6181 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa 6241 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt 6301 tgtccaaact catcaatgta tcttatcatg tctggatccc ttgaaataca tccacaggta 6361 cacctccaat tgactcaaat gatgtcaatt agtctatcag aagcttgtgg aaggctactc 6421 gaaatgtttg acccaagtta aacaatttaa aggcaatgct accaaatact aattgagtgt 6481 atgtaaactt ctgacccact gggaatgtga tgaaagaaat aaaagctgaa atgaatcatt 6541 ctctctacta ttattctgat atttcacatt cttaaaataa agtggtgatc ctaactgacc 6601 taagacaggg aatttttact aggattaaat gtcaggaatt gtgaaaaagt gagtttaaat 6661 gtatttggct aaggtgtatg taaacttccg acttcaactg tatagggatc ctctagctag 6721 agtcgacctc gagggggggc ccggtaccca gcttttgttc cctttagtga gggttaattg 6781 cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa 6841 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga 6901 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt 6961 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct 7021 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 7081 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 7141 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 7201 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 7261 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 7321 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 7381 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 7441 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 7501 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 7561 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 7621 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta 7681 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 7741 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 7801 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 7861 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 7921 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 7981 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 8041 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 8101 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 8161 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 8221 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 8281 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 8341 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 8401 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 8461 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 8521 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 8581 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 8641 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 8701 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 8761 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 8821 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 8881 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 8941 aagtgccacc tgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc 9001 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt 9061 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag 9121 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt 9181 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt 9241 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt 9301 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt 9361 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttcc attcgccatt 9421 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct 9481 ggcgaaaggg ggatgtgctg //