LOCUS Exported 1137 bp ds-DNA linear SYN 17-MAY-2021 DEFINITION Constitutive expression of Cas9, inducible expression of the Red recombineering system, and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.. ACCESSION . VERSION . KEYWORDS pREDCas9 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1137) AUTHORS Li Y, Lin Z, Huang C, Zhang Y, Wang Z, Tang YJ, Chen T, Zhao X TITLE Metabolic engineering of Escherichia coli using CRISPR-Cas9 meditated genome editing. JOURNAL Metab Eng. 2015 Sep;31:13-21. doi: 10.1016/j.ymben.2015.06.006. Epub 2015 Jun 30. PUBMED 26141150 REFERENCE 2 (bases 1 to 1137) AUTHORS . TITLE Direct Submission JOURNAL Exported May 17, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1137 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(415..1095) /codon_start=1 /gene="exo" /product="5' to 3' double-stranded DNA exonuclease in the lambda Red system" /label=Exo /note="creates 3' single-stranded DNA tails" /translation="MTPDIILQRTGIDVRAVEQGEDAWHKLRLGVITASEVHNVIAKPR SGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTESP IIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQYSMW VTRKNAWYFANYDPRMKREGLHYVVIERDEKYMASFDEIVPEFIEKMDEALAEIGFVFG EQWR" ORIGIN 1 agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa 61 aggcgagatc accaaggtag tcggcaaata atgtctaaca attcgttcaa gccgacgccg 121 cttcgcggcg cggcttaact caagcgttag atgcactaag cacataattg ctcacagcca 181 aactatcagg tcaagtctgc ttttattatt tttaagcgtg cataataagc cctacacaaa 241 ttgggagata tatcatgaaa ggctggcttt ttcttgttat cgcaatagtt ggcgaagtaa 301 tcgcaacatc cgcattaaaa tctagcgagg gctttactaa gctcctgcag agatctgaat 361 tccctagaga gacgaaagtg attgcgccta cccggatatt atcgtgagga tgcgtcatcg 421 ccattgctcc ccaaatacaa aaccaatttc agccagtgcc tcgtccattt tttcgatgaa 481 ctccggcacg atctcgtcaa aactcgccat gtacttttca tcccgctcaa tcacgacata 541 atgcaggcct tcacgcttca tacgcgggtc atagttggca aagtaccagg cattttttcg 601 cgtcacccac atgctgtact gcacctgggc catgtaagct gactttatgg cctcgaaacc 661 accgagccgg aacttcatga aatcccggga ggtaaacggg catttcagtt caaggccgtt 721 gccgtcactg cataaaccat cgggagagca ggcggtacgc atactttcgt cgcgatagat 781 gatcggggat tcagtaacat tcacgccgga agtgaattca aacagggttc tggcgtcgtt 841 ctcgtactgt tttccccagg ccagtgcttt agcgttaact tccggagcca caccggtgca 901 aacctcagca agcagggtgt ggaagtagga cattttcatg tcaggccact tctttccgga 961 gcggggtttt gctatcacgt tgtgaacttc tgaagcggtg atgacgccga gccgtaattt 1021 gtgccacgca tcttccccct gttcgacagc tctcacatcg atcccggtac gctgcaggat 1081 aatgtccggt gtcatgctgc caccttctgc tctgcggctt tctgtttcag gaatcca //