LOCUS       Exported                 862 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  Can be used to drive expression in zebrafish microglia..
ACCESSION   .
VERSION     .
KEYWORDS    p5E-mpeg1.1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Don EK, Formella I, Badrock AP, Hall TE, Morsch M, Hortle E, Hogan 
            A, Chow S, Gwee SS, Stoddart JJ, Nicholson G, Chung R, Cole NJ
  TITLE     A Tol2 Gateway-Compatible Toolbox for the Study of the Nervous 
            System and Neurodegenerative Disease.
  JOURNAL   Zebrafish. 2016 Sep 15.
  PUBMED    27631880
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..862
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    complement(68..192)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        192..210
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     247..263
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     247..263
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tcgcggccgc cctgcagctc tagagctcga attctacagg tcactaatac catctaagta
       61 gttggttcat agtgactgca tatgttgtgt tttacagtat tatgtagtct gttttttatg
      121 caaaatctaa tttaatatat tgatatttat atcattttac gtttctcgtt caactttttt
      181 gtacaaactt gaattaaccc tcactaaagg gaacaaaagc tggagctcca ccgcggtggc
      241 ggccgctcta gaactagtgg atccttttgc tgtctcctgc actaatgtga ttttcaatca
      301 aatatggtga ttatagattt tgctgagttc ctcaataaaa ccacttctca gtttcaaatc
      361 cgtgggggaa gggtcatgag actatgaggt tcctctttgt gttttggtac cttgcgttca
      421 aacgttaagt tgaaggacaa tttcatgaag tgaatgaatc aaatatattt atattttttg
      481 tgaagtatga tctgttcaat aatagattta tatttaaagc tacgaaggat tttgatgagt
      541 cttcagaatg gttttgatga cgcaagagac tgtgcgtttt aaattttcca ttggctttga
      601 aagaggagga attgaaattg gttggcttct tgttttagaa atgtacaaga ttagcaggtt
      661 tttcaaatcc tctttattat aagcatctcc taaaactgct gtgagcatag gggatcatga
      721 aagccctata gcagaatttt cttcaaaaaa ataagtcggt acaacaatga atccttattt
      781 cctttcactt tttttgttct ctccactttt aaaaaaacat cagctgcact gacattcaat
      841 tatttttcag taaaaacatt ta
//
