LOCUS       Exported                6825 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Adeno-associated virus plasmid, Cre-dependent FLEX switch, mAPX.
ACCESSION   .
VERSION     .
KEYWORDS    AAV-CAG-DIO-mAPX
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6825)
  AUTHORS   Joesch M, Mankus D, Yamagata M, Shahbazi A, Shalek R, Suissa-Peleg 
            A, Meister M, Lichtman JW, Scheirer WJ, Sanes JR
  TITLE     Reconstruction of genetically identified neurons imaged by 
            serial-section electron microscopy.
  JOURNAL   Elife. 2016 Jul 7;5. pii: e15015. doi: 10.7554/eLife.15015.
  PUBMED    27383271
REFERENCE   2  (bases 1 to 6825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6825
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   12..141
                     /label=AAV2 ITR
     enhancer        156..535
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        537..814
                     /label=chicken beta-actin promoter
     intron          816..1833
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1757..1779
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1841..1860
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    1912..1945
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites (Lee and 
                     Saito, 1988)."
     protein_bind    1996..2029
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     protein_bind    complement(2810..2843)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites (Lee and 
                     Saito, 1988)."
     protein_bind    complement(2894..2927)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    2952..3540
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3005..3025)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3423..3434)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3572..4048
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3771..3790)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4088..4228
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4088..4217
                     /label=AAV2 ITR
     rep_origin      4303..4758
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4390..4409)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4600..4621
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4775..4794)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4894..4916
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4954..4972)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5040..5144
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5145..6005
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5363..5382)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6176..6764
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6665..6684
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgctcgacat tgattattga ctagttatta
      181 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
      241 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
      301 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
      361 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
      421 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
      481 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtcga
      541 ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac ccccaatttt
      601 gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg ggggggggcg
      661 cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga gaggtgcggc
      721 ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg
      781 gcggccctat aaaaagcgaa gcgcgcggcg ggcggggagt cgctgcgacg ctgccttcgc
      841 cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta
      901 ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta gcgcttggtt
      961 taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc
     1021 cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc
     1081 gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt
     1141 gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg
     1201 ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt
     1261 gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc ctccccgagt tgctgagcac
     1321 ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc
     1381 ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc cggggagggc
     1441 tcgggggagg ggcgcggcgg cccccggagc gccggcggct gtcgaggcgc ggcgagccgc
     1501 agccattgcc ttttatggta atcgtgcgag agggcgcagg gacttccttt gtcccaaatc
     1561 tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag
     1621 cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc
     1681 cgtccccttc tccctctcca gcctcggggc tgtccgcggg gggacggctg ccttcggggg
     1741 ggacggggca gggcggggtt cggcttctgg cgtgtgaccg gcggctctag agcctctgct
     1801 aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct ggttattgtg
     1861 ctgtctcatc attttggcaa agaattcgga tcctctagag tcgactccgg aataacttcg
     1921 tataggatac tttatacgaa gttatgcaga atggtagctg gattgtagct gctattagca
     1981 atatgaaacc tcttaataac ttcgtatagc atacattata cgaagttatg gcgcgcccta
     2041 tcatgcctcg gcgaatccca gttcagacag tttcaggtgt gcctcagcat agtcggcgaa
     2101 gaacacatcc tcgtcggctg catacttttc gaccagaggc ctgaacacgc tatctgtcag
     2161 cagggctttg tcagagggca gctgcagcag tccatccttc tcgccggtca gcagttcagt
     2221 aaagtaggaa ttgtcgaaga tcagagggtt gcttgtccag ggtccctcga agcctgagcg
     2281 ttccttatga gcggcgccaa tggtgtgccc tccactcagg gccacgatat cctggtcaga
     2341 cagccccatt gcttttccga acacgtcccg cagatggtcg ctgcccttgg tagcatctgg
     2401 cagtctcccc tctggagggg gttcaggttt gtcctccctt cctgggtgga aagggacctc
     2461 ggggccgccg gtaatctcca cagcgaccac gccggccagc tggtagaagt ctgcgtagga
     2521 gacaatgggg aactgttctt tgattggctc cagcagtctc acagcaatgt ccaggccatt
     2581 gttggcccca tgtgccagtt cagcctggtg cttgatagtc ccaaagggtc cgcctgtttt
     2641 ggtcttggag tcaaatgttc ctgcgctgtg gaaagccagt cgcaggatca gtggggcgca
     2701 tttcttttct gcgatgaatc ccctcagttt cctttttgcc ttctcaatag ccttctggta
     2761 atctgggctc acggttgggt atgactttcc cctcatggtg gcggctagca taacttcgta
     2821 taaagtatcc tatacgaagt tatttgcctt aacccagaaa ttatcactgt tattctttag
     2881 aatggtgcaa agaataactt cgtataatgt atgctatacg aagttatgaa ttcgatatca
     2941 agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     3001 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     3061 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     3121 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     3181 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     3241 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     3301 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     3361 cttggctgct cgcctatgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     3421 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     3481 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     3541 atcgataccg agcgctgctc gagagatcta cgggtggcat ccctgtgacc cctccccagt
     3601 gcctctcctg gccctggaag ttgccactcc agtgcccacc agccttgtcc taataaaatt
     3661 aagttgcatc attttgtctg actaggtgtc cttctataat attatggggt ggaggggggt
     3721 ggtatggagc aaggggcaag ttgggaagac aacctgtagg gcctgcgggg tctattggga
     3781 accaagctgg agtgcagtgg cacaatcttg gctcactgca atctccgcct cctgggttca
     3841 agcgattctc ctgcctcagc ctcccgagtt gttgggattc caggcatgca tgaccaggct
     3901 cagctaattt ttgttttttt ggtagagacg gggtttcacc atattggcca ggctggtctc
     3961 caactcctaa tctcaggtga tctacccacc ttggcctccc aaattgctgg gattacaggc
     4021 gtgaaccact gctcccttcc ctgtccttct gattttgtag gtaaccacgt gcggaccgag
     4081 cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc
     4141 actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg
     4201 agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat
     4261 ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg
     4321 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     4381 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     4441 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     4501 accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     4561 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     4621 gaacaacact caaccctatc tcgggctatt cttttgattt ataagggatt ttgccgattt
     4681 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     4741 tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     4801 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     4861 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     4921 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
     4981 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     5041 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     5101 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     5161 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     5221 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     5281 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     5341 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     5401 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     5461 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     5521 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     5581 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     5641 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     5701 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     5761 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     5821 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     5881 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     5941 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     6001 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     6061 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     6121 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     6181 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     6241 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     6301 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     6361 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     6421 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     6481 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     6541 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     6601 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     6661 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     6721 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     6781 cctttttacg gttcctggcc ttttgctggc cttttgctca catgt
//
