LOCUS       Exported                 823 bp ds-DNA     linear   SYN 12-JUN-2021
DEFINITION  To overexpress p21 90-164 in Mammalian Cell.
ACCESSION   .
VERSION     .
KEYWORDS    pCS3-MT-BX-6MYC p21 90-164
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Lee MS, Seo J, Choi DY, Lee EW, Ko A, Ha NC, Yoon JB, Lee HW, Kim 
            KP, Song J
  TITLE     Stabilization of p21 (Cip1/WAF1) following Tip60-dependent 
            acetylation is required for p21-mediated DNA damage response.
  JOURNAL   Cell Death Differ. 2013 Apr;20(4):620-9. doi: 10.1038/cdd.2012.159. 
            Epub 2012 Dec 14.
  PUBMED    23238566
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        37..55
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     37..54
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             105..134
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             144..173
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             183..212
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             222..251
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             261..290
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             324..353
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        complement(661..678)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    683..817
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(707..726)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     761..780
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 accgccattc tgcctgggga cgtcggagca agcttgattt aggtgacact atagaataca
       61 agctacttgt tctttttgca ggatcccatc gatttaaagc tatggagcaa aagctcattt
      121 ctgaagagga cttgaatgaa atggagcaaa agctcatttc tgaagaggac ttgaatgaaa
      181 tggagcaaaa gctcatttct gaagaggact tgaatgaaat ggagcaaaag ctcatttctg
      241 aagaggactt gaatgaaatg gagcaaaagc tcatttctga agaggacttg aatgaaatgg
      301 agagcttggg cgacctcacc atggagcaaa agctcatttc tgaagaggac ttgaatttag
      361 agatctcgag ctcaagcttc gaattcatgt tgggaggagg caggcggcct ggcacctcac
      421 ctgctctgct gcaggggaca gcagaggaag accatgtgga cctgtcactg tcttgtaccc
      481 ttgtgcctcg ctcaggggag caggctgaag ggtccccagg tggacctgga gactctcagg
      541 gtcgaaaacg gcggcagacc agcatgacag atttctacca ctccaaacgc cggctgatct
      601 tctccaagag gaagccctaa ctcgacggta ccgcgggccc gggatccacc ggatctagaa
      661 ctatagtgag tcgtattacg tagatccaga catgataaga tacattgatg agtttggaca
      721 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
      781 tttatttgta accattataa gctgcaataa acaagtttac caa
//
