LOCUS       Exported                 895 bp ds-DNA     linear   SYN 26-MAY-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    Flag-mPIAS1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Liu B, Liao J, Rao X, Kushner SA, Chung CD, Chang DD, Shuai K
  TITLE     Inhibition of Stat1-mediated gene activation by PIAS1.
  JOURNAL   Proc Natl Acad Sci U S A. 1998 Sep 1. 95(18):10626-31.
  PUBMED    9724754
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 26, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      20..29
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             29..52
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ttagtgaacc gtcagaattg ccaccatgga ctacaaggac gacgatgaca agaattctgc
       61 aggatcccgg gtacctctag agatctggaa ttcccgggag atggcggaca gtgcggaact
      121 aaagcaaatg gttatgagcc ttagagtttc tgaactccaa gtactgttgg gctacgctgg
      181 gaggaacaag cacggacgca aacacgaact tcttacaaaa gccctgcatt tgttaaaggc
      241 tggctgtagt cctgctgtac aaatgaaaat taaagaactc tacaggaggc ggttccctca
      301 gaaaattatg acgcctgcgg acttgtctat ccccaacgta cattcaagtc ctatgcctcc
      361 gactctttct ccatccacca ttccacagct cacttatgat ggccaccctg catcatcccc
      421 actactccct gtttctcttc tgggacccaa acatgaactg gaactcccac atctcacgtc
      481 agcgctgcac ccagtccacc cggacataaa gctgcagaag ctaccattct atgacctgtt
      541 ggatgaactg atcaagccca ccagtctagc ttcagacaac agccagcgct ttcgggaaac
      601 ctgttttgca tttgccttga caccacaaca ggtgcagcag atcagcagct ccatggatat
      661 ttctgggacc aaatgtgact tcacagtgca ggtccaatta aggttttgtt tatcagaaac
      721 cagttgtcca caagaagatc acttcccacc caacctttgt gtaaaagtga atacaaaacc
      781 ttgcagcctt ccaggttacc ttccacctac taaaaacggt gtggaaccaa agcgacctag
      841 ccgaccaatt aatatcacct cacttgtccg attgtccacg acagtaccaa atacc
//
