LOCUS Exported 2978 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION miRFP709 GAF domain fused to FKBP and NLS. ACCESSION . VERSION . KEYWORDS pC4EN-F1-mGAF709 (NLS-FKBP-mGAF709) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2978) AUTHORS Shcherbakova DM, Baloban M, Emelyanov AV, Brenowitz M, Guo P, Verkhusha VV TITLE Bright monomeric near-infrared fluorescent proteins as tags and biosensors for multiscale imaging. JOURNAL Nat Commun. 2016 Aug 19;7:12405. doi: 10.1038/ncomms12405. PUBMED 27539380 REFERENCE 2 (bases 1 to 2978) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2978 /organism="synthetic DNA construct" /mol_type="other DNA" intron 649..1221 /label=-beta-globin intron /note="intron from rabbit beta-globin gene" enhancer 1916..2219 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 2220..2423 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 2374..2394 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 2420..2444 /label=LNCX /note="Human CMV promoter, forward primer" 5'UTR 2513..2552 /label=HSV TK 5'-UTR /note="5' untranslated region from the herpes simplex virus thymidine kinase gene" CDS 2582..2608 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" CDS 2630..2650 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 2657..2977 /codon_start=1 /product="human FK506-binding protein FKBP12" /label=FKBP /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL KLE" ORIGIN 1 ctagtggagg cgggggaagc ggcggaggcg ggtcccgtgc cggcccgtcg atcgatctgt 61 caggcacgct ggcgccggcg ctggagcgga tccgcacggc gggttcactg cgcgcgctgt 121 gcgatgacac cgtgctgctg tttcagcagt gcaccggcta cgaccgggtg atggtgtatc 181 gtttcgatga gcaaggccac ggcctggtat tctccgagtg ccatgtgcct gggctcgaat 241 cctatttcgg caaccgctat ccgtcgtcgt tcatcccgca gatggcgcgg cagctgtacg 301 tgcggcagcg cgtccgcgtg ctggtcgacg tcacctatca gccggtgccg ctggagccgc 361 ggctgtcgcc gctgaccggg cgcgatctcg acatgtcggg ctgcttcctg cgctcgatgt 421 cgccgatcca tctgcagttc ctgaaggaca tgggcgtgcg cgccaccctg gcggtgtcgc 481 tggtggtcgg cggcaagctg tggggcctgg ttgtctgtca ccattatctg ccgcgcttca 541 tccgtttcga gctgcgggcg atctgcaaac ggctcgccga aaggatcgcg acgcggatca 601 ccgcgcttga gagctaaact agttattaag gatcctgaga acttcagggt gagtttgggg 661 acccttgatt gttctttctt tttcgctatt gtaaaattca tgttatatgg agggggcaaa 721 gttttcaggg tgttgtttag aatgggaaga tgtcccttgt atcaccatgg accctcatga 781 taattttgtt tctttcactt tctactctgt tgacaaccat tgtctcctct tattttcttt 841 tcattttctg taactttttc gttaaacttt agcttgcatt tgtaacgaat ttttaaattc 901 acttttgttt atttgtcaga ttgtaagtac tttctctaat cacttttttt tcaaggcaat 961 cagggtatat tatattgtac ttcagcacag ttttagagaa caattgttat aattaaatga 1021 taaggtagaa tatttctgca tataaattct ggctggcgtg gaaatattct tattggtaga 1081 aacaactaca tcctggtcat catcctgcct ttctctttat ggttacaatg atatacactg 1141 tttgagatga ggataaaata ctctgagtcc aaaccgggcc cctctgctaa ccatgttcat 1201 gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttgttgtgct gtctcatcat 1261 tttggcaaag gattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg 1321 gccaatgccc tggctcacaa ataccactga gatctttttc cctctgccaa aaattatggg 1381 gacatcatga agcccctatc agaaggtggt ggctggtgtg gccaatgccc tggctcacaa 1441 ataccactga gatctttttc cctctgccaa aaattatggg gacatcatga agcccctaat 1501 catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc catatgctgg 1561 ctgccatgaa caaaggtggc tataaagagg tcatcagtat atgaaacagc cccctgctgt 1621 ccattcctta ttccatagaa aagccttgac ttgaggttag atttttttta tattttgttt 1681 tgtgttattt ttttctttaa catccctaaa attttcctta catgttttac tagccagatt 1741 tttcctcctc tcctgactac tcccagtcat agctgtccct cttctcttat gaagatccct 1801 cgaggagctt tttgcaaaag ccctaggcct ccaaaaaagc ctcttcacta cttctggaat 1861 agctcagagg ccgaggcggc ctcggcctct gcataaaacg cgttcgagct cgccccgtta 1921 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt 1981 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg 2041 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta 2101 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga 2161 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg 2221 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc 2281 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact 2341 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt 2401 gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc 2461 cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg gggatcttgg 2521 tggcgtgaaa ctcccgcacc tcttcggcca gcgaattcca gaagcgcgta tggcttctag 2581 ctatccttat gacgtgcctg actatgccag cctgggagga ccttctagtc ctaagaagaa 2641 gagaaaggtg tctagaggag tgcaggtgga aaccatctcc ccaggagacg ggcgcacctt 2701 ccccaagcgc ggccagacct gcgtggtgca ctacaccggg atgcttgaag atggaaagaa 2761 atttgattcc tcccgggaca gaaacaagcc ctttaagttt atgctaggca agcaggaggt 2821 gatccgaggc tgggaagaag gggttgccca gatgagtgtg ggtcagagag ccaaactgac 2881 tatatctcca gattatgcct atggtgccac tgggcaccca ggcatcatcc caccacatgc 2941 cactctcgtc ttcgatgtgg agcttctaaa actggaaa //