LOCUS       Exported                 605 bp ds-DNA     linear   SYN 03-JUN-2021
DEFINITION  Cre-dependent bicistronic vector expressing mRuby2 and GCaMP6f from 
            a single open reading frame..
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Rose T, Jaepel J, Hubener M, Bonhoeffer T
  TITLE     Cell-specific restoration of stimulus preference after monocular 
            deprivation in the visual cortex.
  JOURNAL   Science. 2016 Jun 10;352(6291):1319-22. doi: 
            10.1126/science.aad3358.
  PUBMED    27284193
REFERENCE   2  (bases 1 to 605)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 3, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    28..61
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     protein_bind    112..145
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites (Lee and 
                     Saito, 1988)."
     regulatory      170..179
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
ORIGIN
        1 atccagaggt tgattatcga taagcttata acttcgtata gcatacatta tacgaagtta
       61 ttctttgcac cattctaaag aataacagtg ataatttctg ggttaaggca aataacttcg
      121 tataggatac tttatacgaa gttatctcga gactagtacc ggtcctaggg ccaccatggt
      181 gtctaagggc gaagagctga tcaaggaaaa tatgcgtatg aaggtggtca tggaaggttc
      241 ggtcaacggc caccaattca aatgcacagg tgaaggagaa ggcaatccgt acatgggaac
      301 tcaaaccatg aggatcaaag tcatcgaggg aggacccctg ccatttgcct ttgacattct
      361 tgccacgtcg ttcatgtatg gcagccgtac ttttatcaag tacccgaaag gcattcctga
      421 tttctttaaa cagtcctttc ctgagggttt tacttgggaa agagttacga gatacgaaga
      481 tggtggagtc gtcaccgtca tgcaggacac cagccttgag gatggctgtc tcgtttacca
      541 cgtccaagtc agaggggtaa actttccctc caatggtccc gtgatgcaga agaagaccaa
      601 gggtt
//
