LOCUS       Exported                 247 bp ds-DNA     linear   SYN 31-MAY-2021
DEFINITION  Gateway destination vector for production of MBP-3C site-His6-TEV 
            site-passenger protein fusions.
ACCESSION   .
VERSION     .
KEYWORDS    pSRK2721
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 247)
  AUTHORS   Raran-Kurussi S, Waugh DS
  TITLE     A dual protease approach for expression and affinity purification of
            recombinant proteins.
  JOURNAL   Anal Biochem. 2016 Apr 19;504:30-37. doi: 10.1016/j.ab.2016.04.006.
  PUBMED    27105777
REFERENCE   2  (bases 1 to 247)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 31, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..247
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        complement(18..120)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgataa ctcaaaaaat
       61 acgcccggta gtgatcttat ttcattatgg tgaaagttgg aacctcttgc gtgccgatca
      121 acgtctcatt ttcgccaaaa gttggcccag ggcttcccgg tatcaacagg gacaccagga
      181 tttatttatt ctgcgaagtg atcttccgtc acaggtattt attcggcgca aagtgcgtcg
      241 ggtgatc
//
