LOCUS Exported 8469 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS Stat3 S727A pRc/CMV SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8469) AUTHORS Wen Z, Darnell JE Jr TITLE Mapping of Stat3 serine phosphorylation to a single residue (727) and evidence that serine phosphorylation has no influence on DNA binding of Stat1 and Stat3. JOURNAL Nucleic Acids Res 1997 Jun 1;25(11):2062-7. PUBMED 9153303 REFERENCE 2 (bases 1 to 8469) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8469 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 102..481 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 482..685 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 636..656 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 730..749 /label=T7 /note="T7 promoter, forward primer" promoter 730..748 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind 839..855 /label=SK primer /note="common sequencing primer, one of multiple similar variants" primer_bind 839..855 /label=pBluescriptSK /note="For pBluescript vector" primer_bind complement(3764..3780) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3765..3781) /label=pBluescriptKS /note="For pBluescript vector" promoter complement(3795..3813) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(3796..3813) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(3833..3850) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 3839..4063 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 4109..4622 /direction=RIGHT /label=M13 ori /note="M13 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4196..4215) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 4406..4427 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(4645..4665) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 4650..4979 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 4830..4965 /label=SV40 ori /note="SV40 origin of replication" primer_bind 4892..4911 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 5046..5840 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(5100..5119) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 5710..5729 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 6014..6148 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(6051..6070) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 6105..6124 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(6179..6195) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6179..6195) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(6192..6214) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 6203..6219 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(6227..6257) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6272..6293 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(6410..6427) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(6581..7169) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(6661..6680) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(7340..8200) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 7963..7982 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(8201..8305) /gene="bla" /label=AmpR promoter primer_bind complement(8380..8399) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" ORIGIN 1 agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg 61 ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag 121 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt 181 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac 241 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg 301 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag 361 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat 421 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat 481 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt 541 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga 601 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg 661 gtgggaggtc tatataagca gagctctctg gctaactaga gaacccactg cttactggct 721 tatcgaaatt aatacgactc actataggga gacccaagct tggtaccgag ctcggatcca 781 ctagtaacgg ccgccagtgt gctggaattc tgcagatatc catcacactg gcggccgctc 841 tagaactagt ggatcccccg ggctgcagga attccgccgc gaccagcgca ggccggccag 901 tcgggcctca gccccggaga cagtcgagac ccctgactgc agcaggatgg ctcagtggaa 961 ccagctgcag cagctggaca cacgctacct gaagcagctg caccagctgt acagcgacag 1021 cttccccatg gagctgcggc agttcctggc accttggatt gagagtcaag actgggcata 1081 tgcagccagc aaagagtcac atgccacgtt ggtgtttcat aatctcttgg gtgaaattga 1141 ccagcaatat agccgattcc tgcaagagtc caatgtcctc tatcagcaca accttcgaag 1201 aatcaagcag tttctgcaga gcaggtatct tgagaagcca atggaaattg cccggatcgt 1261 ggcccgatgc ctgtgggaag agtctcgcct cctccagacg gcagccacgg cagcccagca 1321 agggggccag gccaaccacc caacagccgc cgtagtgaca gagaagcagc agatgttgga 1381 gcagcatctt caggatgtcc ggaagcgagt gcaggatcta gaacagaaaa tgaaggtggt 1441 ggagaacctc caggacgact ttgatttcaa ctacaaaacc ctcaagagcc aaggagacat 1501 gcaggatctg aatggaaaca accagtctgt gaccagacag aagatgcagc agctggaaca 1561 gatgctcaca gccctggacc agatgcggag aagcattgtg agtgagctgg cggggctctt 1621 gtcagcaatg gagtacgtgc agaagacact gactgatgaa gagctggctg actggaagag 1681 gcggcagcag atcgcgtgca tcggaggccc tcccaacatc tgcctggacc gtctggaaaa 1741 ctggataact tcattagcag aatctcaact tcagacccgc caacaaatta agaaactgga 1801 ggagctgcag cagaaagtgt cctacaaggg cgaccctatc gtgcagcacc ggcccatgct 1861 ggaggagagg atcgtggagc tgttcagaaa cttaatgaag agtgccttcg tggtggagcg 1921 gcagccctgc atgcccatgc acccggaccg gcccttagtc atcaagactg gtgtccagtt 1981 taccacgaaa gtcaggttgc tggtcaaatt tcctgagttg aattatcagc ttaaaattaa 2041 agtgtgcatt gataaagact ctggggatgt tgctgccctc agagggtctc ggaaatttaa 2101 cattctgggc acgaacacaa aagtgatgaa catggaggag tctaacaacg gcagcctgtc 2161 tgcagagttc aagcacctga cccttaggga gcagagatgt gggaatggag gccgtgccaa 2221 ttgtgatgcc tccttgatcg tgactgagga gctgcacctg atcaccttcg agactgaggt 2281 gtaccaccaa ggcctcaaga ttgacctaga gacccactcc ttgccagttg tggtgatctc 2341 caacatctgt cagatgccaa atgcttgggc atcaatcctg tggtataaca tgctgaccaa 2401 taaccccaag aacgtgaact tcttcactaa gccgccaatt ggaacctggg accaagtggc 2461 cgaggtgctc agctggcagt tctcgtccac caccaagcga gggctgagca tcgagcagct 2521 gacaacgctg gctgagaagc tcctagggcc tggtgtgaac tactcagggt gtcagatcac 2581 atgggctaaa ttctgcaaag aaaacatggc tggcaagggc ttctccttct gggtctggct 2641 agacaatatc atcgaccttg tgaaaaagta tatcttggcc ctttggaatg aagggtacat 2701 catgggtttc atcagcaagg agcgggagcg ggccatccta agcacaaagc ccccgggcac 2761 cttcctactg cgcttcagcg agagcagcaa agaaggaggg gtcactttca cttgggtgga 2821 aaaggacatc agtggcaaga cccagatcca gtctgtagag ccatacacca agcagcagct 2881 gaacaacatg tcatttgctg aaatcatcat gggctataag atcatggatg cgaccaacat 2941 cctggtgtct ccacttgtct acctctaccc cgacattccc aaggaggagg catttggaaa 3001 gtactgtagg cccgagagcc aggagcaccc cgaagccgac ccaggtagtg ctgccccgta 3061 cctgaagacc aagttcatct gtgtgacacc aacgacctgc agcaatacca ttgacctgcc 3121 gatggccccc cgcactttag attcattgat gcagtttgga aataacggtg aaggtgctga 3181 gccctcagca ggagggcagt ttgagtcgct cacgtttgac atggatctga cctcggagtg 3241 tgctacctcc cccatgtgag gagctgaaac cagaagctgc agagacgtga cttgagacac 3301 ctgccccgtg ctccacccct aagcagccga accccatatc gtctgaaact cctaactttg 3361 tggttccaga tttttttttt taatttccta cttctgctat ctttgggcaa tctgggcact 3421 ttttaaaata gagaaatgag tgagtgtggg tgataaacta ttatgtaaag aggagagcac 3481 ctctgagtct ggggatgggg ctgagagcag aagggagcaa ggggaacacc tcctgtcctg 3541 cccgcctgcc ctcctttttc agcagctcgg ggttggttgt tagacaagtg cctcctggtg 3601 cccatggcat cctgttgccc cactctgtga gctgataccc caggctggga actcctggct 3661 ctgcactttc aaccttgcta atatccacat agaagctagg actaagccca gaggttcctc 3721 tttaaattaa aaaaaaaaaa aaggaattcg atatcaagct tatcgatacc gtcgacctcg 3781 agggggggcc ctattctata gtgtcaccta aatgctagag ctcgctgatc agcctcgact 3841 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg 3901 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg 3961 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg 4021 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga 4081 accagctggg gctcgagggg ggatccccac gcgccctgta gcggcgcatt aagcgcggcg 4141 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct 4201 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat 4261 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt 4321 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg 4381 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac 4441 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta 4501 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca 4561 atttaaatat ttgcttatac aatcttcctg tttttggggc ttttctgatt atcaaccggg 4621 gtgggtaccg agctcgaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag 4681 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg 4741 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag 4801 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc 4861 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg 4921 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa 4981 agctcccggg agcttggata tccattttcg gatctgatca agagacagga tgaggatcgt 5041 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc 5101 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc 5161 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg 5221 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag 5281 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg 5341 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg 5401 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac 5461 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg 5521 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc 5581 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg 5641 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc 5701 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc 5761 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc 5821 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc 5881 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg 5941 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt 6001 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat 6061 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact 6121 catcaatgta tcttatcatg tctggatccg tcgacctcga gagcttggcg taatcatggt 6181 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg 6241 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt 6301 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg 6361 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg 6421 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 6481 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 6541 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 6601 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 6661 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 6721 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 6781 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 6841 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 6901 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 6961 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 7021 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 7081 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 7141 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 7201 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 7261 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 7321 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 7381 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 7441 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 7501 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 7561 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 7621 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 7681 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 7741 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 7801 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 7861 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 7921 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 7981 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 8041 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 8101 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 8161 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 8221 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 8281 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg 8341 gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc 8401 atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag 8461 caaaattta //