LOCUS Exported 6694 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION mammalian expression of mouse JunD. ACCESSION . VERSION . KEYWORDS pBabe JunD-HA neo SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6694) AUTHORS Wang CC, Bajikar SS, Jamal L, Atkins KA, Janes KA TITLE A time- and matrix-dependent TGFBR3-JUND-KRT5 regulatory circuit in single breast epithelial cells and basal-like premalignancies. JOURNAL Nat Cell Biol. 2014 Apr;16(4):345-56. doi: 10.1038/ncb2930. Epub 2014 Mar 23. PUBMED 24658685 REFERENCE 2 (bases 1 to 6694) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6694 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 8..477 /note="long terminal repeat from Moloney murine leukemia virus" misc_feature 542..741 /label=MMLV Psi /note="packaging signal of Moloney murine leukemia virus (MMLV)" CDS 934..1350 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" primer_bind 1280..1302 /label=pLXSN 5' /note="Murine stem cell virus, forward primer. Also called MSCV" primer_bind 1318..1334 /label=pBABE 5' /note="Psi packaging signal, forward primer for pBABE vectors" CDS complement(1367..1378) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" CDS 2384..2410 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" primer_bind complement(2443..2463) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 2448..2777 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 2628..2763 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2690..2709 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 3134..3928 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(3188..3207) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3798..3817 /label=Neo-F /note="Neomycin resistance gene, forward primer" LTR 3975..4568 /note="long terminal repeat from Moloney murine leukemia virus" primer_bind complement(4769..4786) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(4940..5528) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(5020..5039) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(5706..6566) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 6329..6348 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(6567..6671) /gene="bla" /label=AmpR promoter ORIGIN 1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca 61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt 121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag 181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc 241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga 301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc 361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc 421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt 481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg 541 taagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta 601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa 661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc 721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg 781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt 841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt 901 ctgactgtgt ttctgtattt gtctgaaaat tagggccaga ctgttaccac tcccttaagt 961 ttgaccttag atcactggaa agatgtcgag cggctcgctc acaaccagtc ggtagatgtc 1021 aagaagagac gttgggttac cttctgctct gcagaatggc caacctttaa cgtcggatgg 1081 ccgcgagacg gcacctttaa ccgagacctc atcacccagg ttaagatcaa ggtcttttca 1141 cctggcccgc atggacaccc agaccaggtc ccctacatcg tgacctggga agccttggct 1201 tttgaccccc ctccctgggt caagcccttt gtacacccta agcctccgcc tcctcttcct 1261 ccatccgccc cgtctctccc ccttgaacct cctcgttcga ccccgcctcg atcctccctt 1321 tatccagccc tcactccttc tctaggcgcc ggccggatcc atggaaacgc ccttctatgg 1381 cgaggaggcg ctgagcggcc tggctgcggg tgcgtcgagc gtcgctggtg ctactggggc 1441 ccccggcggt ggtggcttcg cgcccccggg ccgcgctttc cccggggcgc ccccgacgag 1501 cagcatgctg aagaaagacg cgctgacgct cagcctggcg gagcagggag cggcgggatt 1561 gaaaccaggg tcggccactg caccttctgc gctgcgcccc gacggcgccc ccgacgggct 1621 gctggcttcg ccggatcttg ggctgctcaa actcgcgtcg ccggagctgg agaggctgat 1681 catccagtcc aacgggctgg tgaccactac cccgaccagt acgcagttcc tctacccgaa 1741 ggtggcagcc agcgaggagc aggagttcgc cgaaggcttc gtcaaggcgc tggaggacct 1801 gcacaagcaa agccagctgg gtgcggccac cgcggccacc tcaggggctc ccgcgcctcc 1861 cgcgcccgcc gacctggccg ccacccccgg ggccacggag accccggtct acgccaacct 1921 gagcagtttc gcgggtggcg ccgggccccc tgggggcgcg gccaccgtgg ctttcgccgc 1981 ggagccagtg cccttcccgc cgcccccggg cgcgctgggg ccgccgccac ctccgcatcc 2041 accgcgcctg gccgcgctca aggacgagcc gcagaccgtg ccggacgtgc cgagcttcgg 2101 cgacagccct ccgctgtcgc ccatcgacat ggacacgcaa gaacgcatca aggcggagcg 2161 caagaggctg cgcaaccgca tcgccgcctc caaatgccgc aagcgcaagc tggagcgtat 2221 ctcgcgcctg gaggagaaag tcaagaccct caaaagccag aacaccgagc tggcgtccac 2281 cgccagcctg ctgcgcgagc aggtggcgca gctcaaacag aaagtcctca gccacgtcaa 2341 cagcggctgc cagctgctgc cccagcacca ggtcccggcg tactacccat acgacgtccc 2401 agactacgct taagaattcg ccagcacagt ggtcgaccct gtggaatgtg tgtcagttag 2461 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt 2521 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca 2581 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa 2641 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag 2701 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag 2761 gcctaggctt ttgcaaaaag cttcacgctg ccgcaagcac tcagggcgca agggctgcta 2821 aaggaagcgg aacacgtaga aagccagtcc gcagaaacgg tgctgacccc ggatgaatgt 2881 cagctactgg gctatctgga caagggaaaa cgcaagcgca aagagaaagc aggtagcttg 2941 cagtgggctt acatggcgat agctagactg ggcggtttta tggacagcaa gcgaaccgga 3001 attgccagct ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa actggatggc 3061 tttcttgccg ccaaggatct gatggcgcag gggatcaaga tctgatcaag agacaggatg 3121 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt 3181 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt 3241 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc 3301 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc 3361 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga 3421 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat 3481 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca 3541 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga 3601 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc 3661 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat 3721 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga 3781 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg 3841 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt 3901 ctatcgcctt cttgacgagt tcttctgaga tcgataaaat aaaagatttt atttagtctc 3961 cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc ttaagtaacg 4021 ccattttgca aggcatggaa aaatacataa ctgagaatag agaagttcag atcaaggtca 4081 ggaacagatg gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc 4141 cccggctcag ggccaagaac agatggaaca gctgaatatg ggccaaacag gatatctgtg 4201 gtaagcagtt cctgccccgg ctcagggcca agaacagatg gtccccagat gcggtccagc 4261 cctcagcagt ttctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga 4321 ccctgtgcct tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct 4381 gctccccgag ctcaataaaa gagcccacaa cccctcactc ggggcgccag tcctccgatt 4441 gactgagtcg cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg 4501 tggtctcgct gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt 4561 ctttcatttc cgacttgtgg tctcgctgcc ttgggagggt ctcctctgag tgattgacta 4621 cccgtcagcg ggggtcttca catgcagcat gtatcaaaat taatttggtt ttttttctta 4681 agtatttaca ttaaatggcc atagttgcat taatgaatcg gccaacgcgc ggggagaggc 4741 ggtttgcgta ttggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 4801 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 4861 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 4921 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 4981 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 5041 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 5101 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 5161 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 5221 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 5281 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 5341 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg 5401 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 5461 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 5521 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 5581 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 5641 attaaaaatg aagtttgcgg ccgcaaatca atctaaagta tatatgagta aacttggtct 5701 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 5761 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 5821 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 5881 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 5941 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 6001 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct 6061 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 6121 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 6181 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 6241 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 6301 agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa 6361 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 6421 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 6481 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 6541 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat 6601 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 6661 ggggttccgc gcacatttcc ccgaaaagtg ccac //