LOCUS       Exported                 830 bp ds-DNA     linear   SYN 30-MAY-2021
DEFINITION  Mammalian expression of NgAgo (Argonaute from N. gregoryi)..
ACCESSION   .
VERSION     .
KEYWORDS    nls-NgAgo-GK
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Gao F, Shen XZ, Jiang F, Wu Y, Han C
  TITLE     DNA-guided genome editing using the Natronobacterium gregoryi 
            Argonaute.
  JOURNAL   Nat Biotechnol. 2016 May 2. doi: 10.1038/nbt.3547.
  PUBMED    27136078
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 30, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      57..66
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             69..89
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ttagtgaacc gtcagatccg ctagcgctac cggactcaga tctcgagctc aagcttgcca
       61 ccatggtgcc aaaaaagaag agaaaggtag ccacagtgat tgacctcgat tcgaccacca
      121 ccgcagacga actgacatcg ggacacacgt acgacatctc agtcacgctc accggtgtct
      181 acgataacac cgacgagcag catcctcgca tgtctctcgc attcgagcag gacaacggcg
      241 agcggcgtta cattaccctg tggaagaaca cgacacccaa ggatgtcttt acatacgact
      301 acgccacggg ctcgacgtac atcttcacta acatcgacta cgaagtgaag gacggctacg
      361 agaatctgac tgcaacatac cagacgaccg tcgagaacgc taccgctcag gaagtcggga
      421 cgactgacga ggacgaaacg ttcgcgggcg gcgagccgct cgaccatcac ttggacgacg
      481 cgctcaatga gacgccagac gacgcggaga cagagagcga ctcaggccat gtgatgacct
      541 cgttcgcctc ccgcgaccaa ctccctgagt ggacgctgca tacgtatacg ctaacagcca
      601 cagacggcgc aaagacggac acggagtacg cgcgacgaac cctcgcatac acggtacggc
      661 aggaactcta taccgaccat gatgcggctc cggttgcaac tgacgggcta atgcttctca
      721 cgccagagcc gctcggcgag accccgcttg acctcgattg cggtgtccgg gtcgaggcgg
      781 acgagactcg gacactcgat tacaccacgg ccaaagaccg gttactcgcc
//
