LOCUS       Exported                 952 bp ds-DNA     linear   SYN 24-MAY-2021
DEFINITION  Introduce sgRNA into a lentiviral vector (LentiCRISPR V2) which 
            contains eSpCas9 and puromycin cassette.
ACCESSION   .
VERSION     .
KEYWORDS    pLentiCRISPR-E
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Sikder RK, Ellithi M, Uzzo RN, Weader D, Metz AL, Behbahani A, 
            McKenzie ER, El-Deiry WS, Abbosh PH
  TITLE     Differential effects of clinically-relevant N- vs C-terminal 
            truncating CDKN1A mutations on cisplatin sensitivity in bladder 
            cancer.
  JOURNAL   Mol Cancer Res. 2020 Dec 3. pii: 1541-7786.MCR-19-1200. doi: 
            10.1158/1541-7786.MCR-19-1200.
  PUBMED    33272936
REFERENCE   2  (bases 1 to 952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 24, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..952
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        92..332
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     primer_bind     92..112
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     263..282
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
       61 gcagagatcc agtttggtta attaaggtac cgagggccta tttcccatga ttccttcata
      121 tttgcatata cgatacaagg ctgttagaga gataattaga attaatttga ctgtaaacac
      181 aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt
      241 tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga
      301 tttcttggct ttatatatct tgtggaaagg acgaaacacc ggagacggtt gtaaatgagc
      361 acacaaaata cacatgctaa aatattatat tctatgacct ttataaaatc aaccaaaatc
      421 ttctttttaa taactttagt atcaataatt agaattttta tgttcctttt tgcaaacttt
      481 taataaaaat gagcaaaata aaaaaacgct agttttagta actcgcgttg ttttcttcac
      541 ctttaataat agctactcca ccacttgttc ctaagcggtc agctcctgct tcaatcattt
      601 tttgagcatc ttcaaatgtt ctaactccac cagctgcttt aactaaagca ttgtctttaa
      661 caactgactt cattagttta acatcttcaa atgttgcacc tgattttgaa aatcctgttg
      721 atgttttaac aaattctaat ccagcttcaa cagctatttc acaagctttc atgatttctt
      781 cttttgttaa taaacaattt tccataatac atttaacaac atgtgatcca gctgcttttt
      841 ttacagcttt catgtcttct aaaactaatt cataattttt gtcttttaat gcaccaatat
      901 ttaataccat atcaatttct gttgcaccat ctttaattgc ttcagaaact tc
//
