LOCUS Exported 6376 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS Paxillin-pEGFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6376) AUTHORS Laukaitis CM, Webb DJ, Donais K, Horwitz AF TITLE Differential dynamics of alpha 5 integrin, paxillin, and alpha-actinin during formation and disassembly of adhesions in migrating cells. JOURNAL J Cell Biol. 2001 Jun 25. 153(7):1427-40. PUBMED 11425873 REFERENCE 2 (bases 1 to 6376) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6376 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 16..319 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 320..523 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 474..494 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 2271..2280 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 2277..2996 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 2277..2993 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(2322..2343) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(2583..2602) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 2930..2951 /label=EGFP-C /note="EGFP, forward primer" polyA_signal 3119..3240 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3156..3175) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 3210..3229 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(3247..3702) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3384..3405) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 3596..3615 /label=F1ori-R /note="F1 origin, reverse primer" promoter 3729..3833 /gene="bla" /label=AmpR promoter promoter 3835..4192 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(3858..3878) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 4043..4178 /label=SV40 ori /note="SV40 origin of replication" primer_bind 4105..4124 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 4227..5021 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(4281..4300) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 4891..4910 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(5209..5228) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 5253..5300 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 5629..6217 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6118..6137 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa 61 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac 121 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca 181 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg 241 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt 301 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg 361 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg 421 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat 481 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca 541 gatccgctag cgctaccgga ctcagatcca tggacgacct cgatgcctta ctggcagacc 601 tggaatccac cacctcgcat atctctaagc gaccggtgtt tctaacagag gaaacgcctt 661 actcctaccc aactggaaac catacgtacc aggagattgc tgtgccaccg ccggtgcccc 721 cgccgccttc cagtgaggcc ctaaatggca ctgtgattga ccccttagac cagtggcaac 781 ccagcgtgtc cagatacggc caccagcaac ctcagtccca gtctccgata tacagctcta 841 gtgccaaaag ctccagtgcc tctgttccta gagacgggct cagctcccct tctccccgtg 901 ccagtgagga ggaacacgtg tacagcttcc caaacaagca gaagtctgca gaaccatctc 961 ccacaatgac cagcacctcc ctgggcagca acctctcgga actggacagg cttctcctgg 1021 aactgaatgc tgttcaacat aatcccccca gtggcttctc agcagatgaa gtcagcagaa 1081 gcccatcact gcccaatgtg accggacctc actatgtcat cccagagagc agcagctctg 1141 caggagggaa ggctgcaccc cctacaaaag aaaaaccaaa gcggaatggt gggcgtggga 1201 ttgaagatgt gcgtcccagt gtggagagcc tgctggatga gctggagagc tctgtgccaa 1261 gcccagtccc tgcaatcact gtgagccaag gggaggtgag cagcccccag cgtgtcaacg 1321 ccagtcagca gcagacccgt atctctgctt cttcagctac acgagaactg gatgagctga 1381 tggcgtccct ctctgacttt aagttcatgg cacaggggaa agccggcggc agcagctccc 1441 caccatccac caccccaaag cctggcagtc agctggacac catgctggga agtctgcagt 1501 ctgacctgaa caaactgggg gtagcgacgg tcgccaaggg tgtgtgcgga gcctgcaaga 1561 agccgattgc tgggcaggta gttacagcca tggggaagac ctggcaccct gagcacttcg 1621 tctgcaccca ctgccaggag gagattggat cacggaactt ctttgagcgg gatgggcagc 1681 cctactgtga gaaggactat cacaacctct tctctcctcg ctgctactac tgcaatgggc 1741 caatccttga taaagtggtg acggctttgg acaggacgtg gcaccctgaa cactttttct 1801 gtgcccagtg tggagttttc tttggacctg aaggatttca tgagaaggat ggcaaagcct 1861 attgccgcaa ggactacttt gacatgtttg ctcccaagtg tggaggctgt gcccgggcta 1921 tcctggaaaa ttacatttct gccttaaaca ccctgtggca ccctgagtgc tttgtctgtc 1981 gggaatgttt cacaccgttc atcaacggca gcttcttcga gcacgacggg cagccctact 2041 gcgaggtgca ttaccacgag cgccgcggct cgctgtgctc tggctgccag aagcccatca 2101 cgggacgctg catcactgcc atgggcaaga aatttcaccc cgaacacttt gtctgtgcct 2161 tctgcctcaa gcagctcaac aaaggaacct tcaaagaaca gaacgacaag ccctactgtc 2221 agaactgctt tctcaaactc ttctgtggta ccgcgggccc gggatccatc gccaccatgg 2281 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg 2341 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca 2401 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg 2461 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc 2521 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca 2581 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga 2641 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc 2701 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca 2761 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc 2821 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc 2881 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc 2941 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaagcg 3001 gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa 3061 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa 3121 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 3181 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 3241 aggcgtaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca 3301 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga 3361 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg 3421 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat 3481 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag 3541 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga 3601 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa 3661 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg 3721 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc 3781 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg 3841 cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc 3901 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc 3961 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat 4021 agtcccgcgc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc 4081 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga 4141 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc 4201 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc 4261 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct 4321 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg 4381 acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca 4441 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc 4501 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga 4561 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc 4621 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc 4681 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg 4741 ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct 4801 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc 4861 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc 4921 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc 4981 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga 5041 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt 5101 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg 5161 cggggatctc atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa 5221 ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca 5281 cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga 5341 taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca 5401 ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc 5461 catagcctca ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa 5521 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt 5581 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt 5641 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg 5701 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca 5761 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt 5821 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga 5881 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc 5941 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact 6001 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga 6061 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg 6121 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt 6181 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt 6241 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga 6301 ttctgtggat aaccgtatta ccgccatgca ttagttatta atagtaatca attacggggt 6361 cattagttca tagccc //