LOCUS Exported 6009 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pDS-RED-C1-Beclin1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6009) AUTHORS Sun Q, Fan W, Chen K, Ding X, Chen S, Zhong Q TITLE Identification of Barkor as a mammalian autophagy-specific factor for Beclin 1 and class III phosphatidylinositol 3-kinase. JOURNAL Proc Natl Acad Sci U S A. 2008 Dec 9. 105(49):19211-6. PUBMED 19050071 REFERENCE 2 (bases 1 to 6009) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6009 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 16..319 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 320..523 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 474..494 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 562..571 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 568..1242 /codon_start=1 /product="improved tetrameric variant of DsRed fluorescent protein" /label=DsRed2 /note="mammalian codon-optimized" /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH LFL" primer_bind complement(748..768) /label=DsRed1-N /note="DsRed1, reverse primer" primer_bind 1160..1183 /label=DsRed1-C /note="DsRed1, forward primer" polyA_signal 2752..2873 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2789..2808) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 2843..2862 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(2880..3335) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3017..3038) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 3229..3248 /label=F1ori-R /note="F1 origin, reverse primer" promoter 3362..3466 /gene="bla" /label=AmpR promoter promoter 3468..3825 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(3491..3511) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 3676..3811 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3738..3757 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 3860..4654 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(3914..3933) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 4524..4543 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(4842..4861) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 4886..4933 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 5262..5850 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5751..5770 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa 61 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac 121 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca 181 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg 241 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt 301 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg 361 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg 421 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat 481 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca 541 gatccgctag cgctaccggt cgccaccatg gcctcctccg agaacgtcat caccgagttc 601 atgcgcttca aggtgcgcat ggagggcacc gtgaacggcc acgagttcga gatcgagggc 661 gagggcgagg gccgccccta cgagggccac aacaccgtga agctgaaggt gaccaagggc 721 ggccccctgc ccttcgcctg ggacatcctg tccccccagt tccagtacgg ctccaaggtg 781 tacgtgaagc accccgccga catccccgac tacaagaagc tgtccttccc cgagggcttc 841 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg cgaccgtgac ccaggactcc 901 tccctgcagg acggctgctt catctacaag gtgaagttca tcggcgtgaa cttcccctcc 961 gacggccccg tgatgcagaa gaagaccatg ggctgggagg cctccaccga gcgcctgtac 1021 ccccgcgacg gcgtgctgaa gggcgagacc cacaaggccc tgaagctgaa ggacggcggc 1081 cactacctgg tggagttcaa gtccatctac atggccaaga agcccgtgca gctgcccggc 1141 tactactacg tggacgccaa gctggacatc acctcccaca acgaggacta caccatcgtg 1201 gagcagtacg agcgcaccga gggccgccac cacctgttcc tgagatctcg agctcaagct 1261 tcgaattcta tggaagggtc taagacgtcc aacaacagca ccatgcaggt gagcttcgtg 1321 tgccagcgct gcagccagcc cctgaaactg gacacgagtt tcaagatcct ggaccgtgtc 1381 accatccagg aactcacagc tccattactt accacagccc aggcgaaacc aggagagacc 1441 caggaggaag agactaactc aggagaggag ccatttattg aaactcctcg ccaggatggt 1501 gtctctcgca gattcatccc cccagccagg atgatgtcca cagaaagtgc caacagcttc 1561 actctgattg gggaggcatc tgatggcggc accatggaga acctcagccg aagactgaag 1621 gtcactgggg acctttttga catcatgtcg ggccagacag atgtggatca cccactctgt 1681 gaggaatgca cagatactct tttagaccag ctggacactc agctcaacgt cactgaaaat 1741 gagtgtcaga actacaaacg ctgtttggag atcttagagc aaatgaatga ggatgacagt 1801 gaacagttac agatggagct aaaggagctg gcactagagg aggagaggct gatccaggag 1861 ctggaagacg tggaaaagaa ccgcaagata gtggcagaaa atctcgagaa ggtccaggct 1921 gaggctgaga gactggatca ggaggaagct cagtatcaga gagaatacag tgaatttaaa 1981 cgacagcagc tggagctgga tgatgagctg aagagtgttg aaaaccagat gcgttatgcc 2041 cagacgcagc tggataagct gaagaaaacc aacgtcttta atgcaacctt ccacatctgg 2101 cacagtggac agtttggcac aatcaataac ttcaggctgg gtcgcctgcc cagtgttccc 2161 gtggaatgga atgagattaa tgctgcttgg ggccagactg tgttgctgct ccatgctctg 2221 gccaataaga tgggtctgaa atttcagaga taccgacttg ttccttacgg aaaccattca 2281 tatctggagt ctctgacaga caaatctaag gagctgccgt tatactgttc tggggggttg 2341 cggtttttct gggacaacaa gtttgaccat gcaatggtgg ctttcctgga ctgtgtgcag 2401 cagttcaaag aagaggttga gaaaggcgag acacgttttt gtcttcccta caggatggat 2461 gtggagaaag gcaagattga agacacagga ggcagtggcg gctcctattc catcaaaacc 2521 cagtttaact ctgaggagca gtggacaaaa gctctcaagt tcatgctgac gaatcttaag 2581 tggggtcttg cttgggtgtc ctcacaattt tataacaaat aaggatccac cggatctaga 2641 taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc 2701 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt 2761 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 2821 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaacgcgta 2881 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt 2941 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat 3001 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa 3061 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta 3121 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc 3181 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc 3241 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac 3301 acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt 3361 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 3421 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag 3481 aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc 3541 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc 3601 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg 3661 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 3721 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc 3781 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atcaagagac 3841 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc 3901 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc 3961 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc 4021 cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg 4081 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt 4141 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc 4201 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga 4261 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga 4321 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct 4381 caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc 4441 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt 4501 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg 4561 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat 4621 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc 4681 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa 4741 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat 4801 ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg gaaggagaca 4861 ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacggtgtt 4921 gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca 4981 ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca 5041 agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc 5101 tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc 5161 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc 5221 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg 5281 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 5341 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 5401 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 5461 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 5521 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 5581 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 5641 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 5701 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc 5761 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 5821 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc 5881 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg 5941 gataaccgta ttaccgccat gcattagtta ttaatagtaa tcaattacgg ggtcattagt 6001 tcatagccc //