LOCUS Exported 188 bp ds-DNA linear SYN 31-MAY-2021 DEFINITION Gateway destination vector for production of MBP-3C site-His6-TEV site-passenger protein fusions. ACCESSION . VERSION . KEYWORDS pSRK2721 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 188) AUTHORS Raran-Kurussi S, Waugh DS TITLE A dual protease approach for expression and affinity purification of recombinant proteins. JOURNAL Anal Biochem. 2016 Apr 19;504:30-37. doi: 10.1016/j.ab.2016.04.006. PUBMED 27105777 REFERENCE 2 (bases 1 to 188) AUTHORS . TITLE Direct Submission JOURNAL Exported May 31, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..188 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 22..39 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" protein_bind 49..173 /gene="mutant version of attR" /label=attR1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" ORIGIN 1 ggagttctgt tccagggtcc gcatcaccat caccatcact acgtaatcac aagtttgtac 61 aaaaaagctg aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg 121 cataaaaaac agactacata atactgtaaa acacaacata tccagtcact atggcggccg 181 cgggtgat //