LOCUS Exported 5267 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Full length RCAN1 expression with CMV promoter. Flag tagged.. ACCESSION . VERSION . KEYWORDS RCAN1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5267) AUTHORS Liu Q, Busby JC, Molkentin JD TITLE Interaction between TAK1-TAB1-TAB2 and RCAN1-calcineurin defines a signalling nodal control point. JOURNAL Nat Cell Biol. 2009 Feb;11(2):154-61. doi: 10.1038/ncb1823. Epub 2009 Jan 11. PUBMED 19136967 REFERENCE 2 (bases 1 to 5267) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5267 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 107..128 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 143..173 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 181..197 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 186..208 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 205..221 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 205..221 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" enhancer 250..629 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 630..833 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 784..804 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 830..854 /label=LNCX /note="Human CMV promoter, forward primer" promoter 1064..1082 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind 1064..1081 /label=SP6 /note="SP6 promoter, forward primer" primer_bind 1095..1114 /label=pMT2-F /note="Synthetic intron, forward primer" CDS 1157..1180 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" regulatory 1803..1812 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" polyA_signal 1816..1950 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1853..1872) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 1907..1926 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" promoter 2019..2376 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(2042..2062) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 2227..2362 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2289..2308 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(2396..2413) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(2396..2412) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2405..2427) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 2625..3080 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2712..2731) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 2922..2943 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(3097..3116) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 3216..3238 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(3276..3294) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 3362..3466 /gene="bla" /label=AmpR promoter CDS 3467..4327 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(3685..3704) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 4498..5086 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4987..5006 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 5240..5257 /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 181 ttgtgagcgg ataacaattt cacacaggaa acagctatga catgattacg aattaattcg 241 agctcgcccg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag 301 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct 361 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc 421 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg 481 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat 541 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 601 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 661 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 721 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 781 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag 841 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc 901 gggaccgatc cagcctccgc ggccgggaac ggtgcattgg aacgcggatt ccccgtgcca 961 agagtgacgt aagtaccgcc tatagagtct ataggcccac ccccttggct tcgttagaac 1021 gcggctacaa ttaatacata accttatgta tcatacacat acgatttagg tgacactata 1081 gaataacatc cactttgcct ttctctccac aggtgtccac tcccaggtcc aactgcacct 1141 cggttctatc gatatggact acaaagacga tgacgacaag cgaattcccc ggggatccat 1201 ggaggaggtg gatctgcagg acctgccgag cgccaccatc gcctgccacc tggacccgcg 1261 cgtgttcgtg gacggcctgt gccgggccaa atttgaatcc ctcttcagaa catatgacaa 1321 ggacaccacc ttccagtatt ttaagagctt caaacgtgtc cggataaact tcagcaaccc 1381 cttatctgca gccgatgcca ggctgcggct gcacaagacc gagttcctgg ggaaggaaat 1441 gaagttgtat tttgctcaga ctttacacat aggaagttca cacctggctc cgcccaatcc 1501 cgacaaacag ttcctcatct cccctccggc ctctcctccc gttggctgga aacaagtaga 1561 agatgccacc cccgtcataa attacgatct tttatatgcc atctccaagc tggggccagg 1621 agagaagtat gaactgcatg cagcgacaga caccactccc agtgtggtgg tccacgtgtg 1681 tgagagtgac caagagaatg aggaggaaga ggaagagatg gagagaatga agagacccaa 1741 gcccaaaatc atccagacac ggagaccgga gtacacaccg atccacctta gctgaaagct 1801 tggccgccat ggcccaactt gtttattgca gcttataatg gttacaaata aagcaatagc 1861 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 1921 ctcatcaatg tatcttatca tgtctggatc gggaattaat tcggcgcagc accatggcct 1981 gaaataacct ctgaaagagg aacttggtta ggtaccttct gaggcggaaa gaaccagctg 2041 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg 2101 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca 2161 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact 2221 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta 2281 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag 2341 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tgttaacagc ttggcactgg 2401 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg 2461 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt 2521 cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc 2581 atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg 2641 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc 2701 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc 2761 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct 2821 cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac 2881 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac 2941 tggaacaaca ctcaacccta tctcgggcta ttcttttgat ttataaggga ttttgccgat 3001 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa 3061 aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata 3121 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct 3181 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt 3241 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata 3301 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt 3361 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 3421 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca 3481 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc 3541 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat 3601 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc 3661 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg 3721 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc 3781 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat 3841 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga 3901 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc 3961 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc 4021 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt 4081 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc 4141 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc 4201 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca 4261 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca 4321 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt 4381 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta 4441 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg 4501 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc 4561 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag 4621 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa 4681 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc 4741 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc 4801 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta 4861 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag 4921 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct 4981 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga 5041 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc 5101 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt 5161 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg 5221 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaag //