LOCUS Exported 864 bp ds-DNA linear SYN 19-MAY-2021 DEFINITION 3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses hygromycin for selection.. ACCESSION . VERSION . KEYWORDS pLKO.1 hygro SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 864) TITLE empty vector REFERENCE 2 (bases 1 to 864) AUTHORS . TITLE Direct Submission JOURNAL Exported May 19, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..864 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 120..360 /label=U6 promoter /note="RNA polymerase III promoter for human U6 snRNA" primer_bind 120..140 /label=hU6-F /note="Human U6 promoter, forward primer" primer_bind 291..310 /label=LKO.1 5' /note="Human U6 promoter, forward primer" misc_feature 431..548 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" ORIGIN 1 ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag 61 tgaacggatc tcgacggtat cgatcacgag actagcctcg agcggccgcc cccttcaccg 121 agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga 181 taattggaat taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa 241 agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata 301 tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac 361 gaaacaccgg tccgcaggta tgcacgcgtg aattctcgac ctcgagacaa atggcagtat 421 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag 481 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc 541 aaaattttcg ggtttattac agggacagca gagatccact ttggccgcgg ctcgaggggg 601 ttggggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg 661 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt 721 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct 781 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa 841 gccgcacgtc tcactagtac cctc //