LOCUS Exported 8436 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION DULIP Tandem Construct used for data normalization. ACCESSION . VERSION . KEYWORDS pPA-RL-FL SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8436) AUTHORS Trepte P, Buntru A, Klockmeier K, Willmore L, Arumughan A, Secker C, Zenkner M, Brusendorf L, Rau K, Redel A, Wanker EE TITLE DULIP: A Dual Luminescence-Based Co-Immunoprecipitation Assay for Interactome Mapping in Mammalian Cells. JOURNAL J Mol Biol. 2015 Oct 23;427(21):3375-88. doi: 10.1016/j.jmb.2015.08.003. Epub 2015 Aug 8. PUBMED 26264872 REFERENCE 2 (bases 1 to 8436) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8436 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(46..65) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 237..616 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 617..820 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 771..791 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 865..884 /label=T7 /note="T7 promoter, forward primer" promoter 865..883 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory 940..949 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 970..1143 /codon_start=1 /product="IgG-binding unit of Staphylococcus aureus protein A" /label=ProtA /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL AEAKKLNDAQAPK" CDS 1147..1317 /codon_start=1 /product="IgG-binding unit of Staphylococcus aureus protein A" /label=ProtA /translation="DNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLLA EAKKLNGAQAPK" CDS 1333..2265 /codon_start=1 /product="Renilla luciferase" /label=hRluc /note="human codon-optimized" /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ" protein_bind 2278..2302 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" regulatory 2305..2314 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 2311..3960 /codon_start=1 /gene="luc+" /product="firefly luciferase" /label=luciferase /note="enhanced luc+ version of the luciferase gene" /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV" primer_bind complement(2377..2395) /label=LucNrev /note="Firefly luciferase, reverse primer" protein_bind complement(3962..3986) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" primer_bind complement(4032..4049) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 4038..4262 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 4308..4736 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4395..4414) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 4605..4626 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(4745..4765) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 4750..5079 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 4930..5065 /label=SV40 ori /note="SV40 origin of replication" primer_bind 4992..5011 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 5146..5940 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(5200..5219) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 5810..5829 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 6114..6235 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(6151..6170) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 6205..6224 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(6284..6300) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6284..6300) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(6297..6319) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 6308..6324 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(6332..6362) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6377..6398 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(6515..6532) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(6686..7271) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(6766..6785) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(7442..8302) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 8065..8084 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(8303..8407) /gene="bla" /label=AmpR promoter ORIGIN 1 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga 61 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg 121 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct 181 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca 241 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata 301 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga 361 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt 421 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt 481 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca 541 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt 601 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt 661 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca 721 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg 781 cggtaggcgt gtacggtggg aggtctatat aagcagagct ctctggctaa ctagagaacc 841 cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc aagctggcta 901 gcgtttaaac ttaagcttgg taccgagctc ggatccgccg ccaccatggt tgcgcaacac 961 gatgaagccg tggacaacaa attcaacaaa gaacaacaaa acgcgttcta tgagatctta 1021 catttaccta acttaaacga agaacaacga aacgccttca tccaaagttt aaaagatgac 1081 ccaagccaaa gcgctaacct tttagcagaa gctaaaaagc taaatgatgc tcaggcgccg 1141 aaagtagaca acaaattcaa caaagaacaa caaaacgcgt tctatgagat cttacattta 1201 cctaacttaa acgaagaaca acgaaacgcc ttcatccaaa gtttaaaaga tgacccaagc 1261 caaagcgcta accttttagc agaagctaaa aagctaaatg gtgctcaggc gccgaaagta 1321 gacgcgaatt cgatggcttc caaggtgtac gaccccgagc aacgcaaacg catgatcact 1381 gggcctcagt ggtgggctcg ctgcaagcaa atgaacgtgc tggactcctt catcaactac 1441 tatgattccg agaagcacgc cgagaacgcc gtgatttttc tgcatggtaa cgctgcctcc 1501 agctacctgt ggaggcacgt cgtgcctcac atcgagcccg tggctagatg catcatccct 1561 gatctgatcg gaatgggtaa gtccggcaag agcgggaatg gctcatatcg cctcctggat 1621 cactacaagt acctcaccgc ttggttcgag ctgctgaacc ttccaaagaa aatcatcttt 1681 gtgggccacg actggggggc ttgtctggcc tttcactact cctacgagca ccaagacaag 1741 atcaaggcca tcgtccatgc tgagagtgtc gtggacgtga tcgagtcctg ggacgagtgg 1801 cctgacatcg aggaggatat cgccctgatc aagagcgaag agggcgagaa aatggtgctt 1861 gagaataact tcttcgtcga gaccatgctc ccaagcaaga tcatgcggaa actggagcct 1921 gaggagttcg ctgcctacct ggagccattc aaggagaagg gcgaggttag acggcctacc 1981 ctctcctggc ctcgcgagat ccctctcgtt aagggaggca agcccgacgt cgtccagatt 2041 gtccgcaact acaacgccta ccttcgggcc agcgacgatc tgcctaagat gttcatcgag 2101 tccgaccctg ggttcttttc caacgctatt gtcgagggag ctaagaagtt ccctaacacc 2161 gagttcgtga aggtgaaggg cctccacttc agccaggagg acgctccaga tgaaatgggt 2221 aagtacatca agagcttcgt ggagcgcgtg ctgaagaacg agcagctcga ggatatcaca 2281 agtttgtaca aaaaagcagg cttcgccacc atggaagacg ccaaaaacat aaagaaaggc 2341 ccggcgccat tctatccgct ggaagatgga accgctggag agcaactgca taaggctatg 2401 aagagatacg ccctggttcc tggaacaatt gcttttacag atgcacatat cgaggtggac 2461 atcacttacg ctgagtactt cgaaatgtcc gttcggttgg cagaagctat gaaacgatat 2521 gggctgaata caaatcacag aatcgtcgta tgcagtgaaa actctcttca attctttatg 2581 ccggtgttgg gcgcgttatt tatcggagtt gcagttgcgc ccgcgaacga catttataat 2641 gaacgtgaat tgctcaacag tatgggcatt tcgcagccta ccgtggtgtt cgtttccaaa 2701 aaggggttgc aaaaaatttt gaacgtgcaa aaaaagctcc caatcatcca aaaaattatt 2761 atcatggatt ctaaaacgga ttaccaggga tttcagtcga tgtacacgtt cgtcacatct 2821 catctacctc ccggttttaa tgaatacgat tttgtgccag agtccttcga tagggacaag 2881 acaattgcac tgatcatgaa ctcctctgga tctactggtc tgcctaaagg tgtcgctctg 2941 cctcatagaa ctgcctgcgt gagattctcg catgccagag atcctatttt tggcaatcaa 3001 atcattccgg atactgcgat tttaagtgtt gttccattcc atcacggttt tggaatgttt 3061 actacactcg gatatttgat atgtggattt cgagtcgtct taatgtatag atttgaagaa 3121 gagctgtttc tgaggagcct tcaggattac aagattcaaa gtgcgctgct ggtgccaacc 3181 ctattctcct tcttcgccaa aagcactctg attgacaaat acgatttatc taatttacac 3241 gaaattgctt ctggtggcgc tcccctctct aaggaagtcg gggaagcggt tgccaagagg 3301 ttccatctgc caggtatcag gcaaggatat gggctcactg agactacatc agctattctg 3361 attacacccg agggggatga taaaccgggc gcggtcggta aagttgttcc attttttgaa 3421 gcgaaggttg tggatctgga taccgggaaa acgctgggcg ttaatcaaag aggcgaactg 3481 tgtgtgagag gtcctatgat tatgtccggt tatgtaaaca atccggaagc gaccaacgcc 3541 ttgattgaca aggatggatg gctacattct ggagacatag cttactggga cgaagacgaa 3601 cacttcttca tcgttgaccg cctgaagtct ctgattaagt acaaaggcta tcaggtggct 3661 cccgctgaat tggaatccat cttgctccaa caccccaaca tcttcgacgc aggtgtcgca 3721 ggtcttcccg acgatgacgc cggtgaactt cccgccgccg ttgttgtttt ggagcacgga 3781 aagacgatga cggaaaaaga gatcgtggat tacgtcgcca gtcaagtaac aaccgcgaaa 3841 aagttgcgcg gaggagttgt gtttgtggac gaagtaccga aaggtcttac cggaaaactc 3901 gacgcaagaa aaatcagaga gatcctcata aaggccaaga agggcggaaa gatcgccgtg 3961 gacccagctt tcttgtacaa agtggtgata tcgcggccgc tctagagggc ccgtttaaac 4021 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc 4081 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga 4141 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga 4201 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat 4261 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag 4321 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag 4381 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt 4441 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca 4501 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata 4561 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca 4621 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc 4681 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt 4741 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt 4801 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca 4861 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta 4921 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga 4981 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag 5041 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata 5101 tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat 5161 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca 5221 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg 5281 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg 5341 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact 5401 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct 5461 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg 5521 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt 5581 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc 5641 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc 5701 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga 5761 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc 5821 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt 5881 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga 5941 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt 6001 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg 6061 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt 6121 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag 6181 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg 6241 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg 6301 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta 6361 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg 6421 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga 6481 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 6541 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 6601 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 6661 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 6721 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 6781 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 6841 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 6901 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 6961 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 7021 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 7081 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta 7141 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 7201 aacaaaccac cgctggtagc ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 7261 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 7321 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 7381 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 7441 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 7501 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 7561 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 7621 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 7681 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 7741 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 7801 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 7861 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 7921 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 7981 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 8041 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc 8101 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 8161 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 8221 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 8281 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 8341 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 8401 ttccgcgcac atttccccga aaagtgccac ctgacg //