LOCUS       Exported               12617 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Gene targeting vector for the mouse Rosa26 locus, including a CAG 
            promoter and loxP flanked stop cassette, for cloning into AsiSI or 
            Mlui.
ACCESSION   .
VERSION     .
KEYWORDS    pR26 CAG AsiSI/MluI
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12617)
  AUTHORS   Chu VT, Weber T, Graf R, Sommermann T, Petsch K, Sack U, Volchkov P,
            Rajewsky K, Kuhn R
  TITLE     Efficient generation of Rosa26 knock-in mice using CRISPR/Cas9 in 
            C57BL/6 zygotes.
  JOURNAL   BMC Biotechnol. 2016 Jan 16;16(1):4. doi: 10.1186/s12896-016-0234-4.
  PUBMED    26772810
REFERENCE   2  (bases 1 to 12617)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12617
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    25..1107
                     /label=Rosa26 left arm
                     /note="left homology arm from intron 1 of the mouse Rosa26 
                     gene (Soriano, 1999)"
     misc_feature    1130..1233
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     intron          1477..1542
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    1589..1723
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    1855..1902
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     enhancer        1917..2296
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2299..2574
                     /label=chicken beta-actin promoter
     intron          2575..3583
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3507..3529
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     protein_bind    3630..3663
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     CDS             3760..4359
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3760..3779)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4256..4276
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    4589..4723
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    4725..4758
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     primer_bind     complement(4849..4865)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4849..4865)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(4879..4896)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4885..5109
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        complement(5153..5170)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    5175..5309
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    5382..9722
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     primer_bind     complement(9772..9792)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9772..9790)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9811..9827)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9811..9827)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9824..9846)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9835..9851
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9859..9889)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9904..9925
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10042..10059)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10213..10801)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10293..10312)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10972..11832)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11595..11614
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11833..11937)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(11963..12418)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12100..12121)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     12312..12331
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12545..12567
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     12559..12576
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     12560..12576
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12586..12605
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        12586..12604
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 caccgcatta ccctgttatc cctagcggca ggccctccga gcgtggtgga gccgttctgt
       61 gagacagccg ggtacgagtc gtgacgctgg aaggggcaag cgggtggtgg gcaggaatgc
      121 ggtccgccct gcagcaaccg gagggggagg gagaagggag cggaaaagtc tccaccggac
      181 gcggccatgg ctcggggggg ggggggcagc ggaggagcgc ttccggccga cgtctcgtcg
      241 ctgattggct tcttttcctc ccgccgtgtg tgaaaacaca aatggcgtgt tttggttggc
      301 gtaaggcgcc tgtcagttaa cggcagccgg agtgcgcagc cgccggcagc ctcgctctgc
      361 ccactgggtg gggcgggagg taggtggggt gaggcgagct ggacgtgcgg gcgcggtcgg
      421 cctctggcgg ggcgggggag gggagggagg gtcagcgaaa gtagctcgcg cgcgagcggc
      481 cgcccaccct ccccttcctc tgggggagtc gttttacccg ccgccggccg ggcctcgtcg
      541 tctgattggc tctcggggcc cagaaaactg gcccttgcca ttggctcgtg ttcgtgcaag
      601 ttgagtccat ccgccggcca gcgggggcgg cgaggaggcg ctcccaggtt ccggccctcc
      661 cctcggcccc gcgccgcaga gtctggccgc gcgcccctgc gcaacgtggc aggaagcgcg
      721 cgctgggggc ggggacgggc agtagggctg agcggctgcg gggcgggtgc aagcacgttt
      781 ccgacttgag ttgcctcaag aggggcgtgc tgagccagac ctccatcgcg cactccgggg
      841 agtggaggga aggagcgagg gctcagttgg gctgttttgg aggcaggaag cacttgctct
      901 cccaaagtcg ctctgagttg ttatcagtaa gggagctgca gtggagtagg cggggagaag
      961 gccgcaccct tctccggagg ggggagggga gtgttgcaat acctttctgg gagttctctg
     1021 ctgcctcctg gcttctgagg accgccctgg gcctgggaga atcccttccc cctcttccct
     1081 cgtgatctgc aactccagtc tttctaggcg cgcccgggct gcagatctgt agggcgcagt
     1141 agtccagggt ttccttgatg atgtcatact tatcctgtcc cttttttttc cacagctcgc
     1201 ggttgaggac aaactcttcg cggtctttcc agtggggatc gacggtatcg ataagctggc
     1261 cgctctagtg gccgtacggg cccacctgcc gggccactta attaaattta aatcacaatt
     1321 ccagctgagc gccggtcgct accattacca gttggtctgg tgtcaaaaat aataataacc
     1381 gggcaggggg gatctgcatg gatctttgtg aaggaacctt acttctgtgg tgtgacataa
     1441 ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta
     1501 taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact
     1561 gatgaatggg agcagtggtg gaatgccaga tccagacatg ataagataca ttgatgagtt
     1621 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     1681 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     1741 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct
     1801 ctacaaatgt ggtatggctg attatgatct gcggccgtgc tagcgcttaa gcttgaagtt
     1861 cctattccga agttcctatt ctctagaaag tataggaact tcggcgcgcc ggatctgaca
     1921 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
     1981 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     2041 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     2101 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     2161 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     2221 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     2281 catcgctatt accatgggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc
     2341 ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg
     2401 gggcgggggg ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg
     2461 gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt
     2521 atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc
     2581 gctgcgttgc cttcgccccg tgccccgctc cgcgccgcct cgcgccgccc gccccggctc
     2641 tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt
     2701 aattagcgct tggtttaatg acggctcgtt tcttttctgt ggctgcgtga aagccttaaa
     2761 gggctccggg agggcccttt gtgcgggggg gagcggctcg gggggtgcgt gcgtgtgtgt
     2821 gtgcgtgggg agcgccgcgt gcggcccgcg ctgcccggcg gctgtgagcg ctgcgggcgc
     2881 ggcgcggggc tttgtgcgct ccgcgtgtgc gcgaggggag cgcggccggg ggcggtgccc
     2941 cgcggtgcgg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg
     3001 gtgagcaggg ggtgtgggcg cggcggtcgg gctgtaaccc ccccctgcac ccccctcccc
     3061 gagttgctga gcacggcccg gcttcgggtg cggggctccg tgcggggcgt ggcgcggggc
     3121 tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg gggccgcctc
     3181 gggccgggga gggctcgggg gaggggcgcg gcggccccgg agcgccggcg gctgtcgagg
     3241 cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc
     3301 tttgtcccaa atctggcgga gccgaaatct gggaggcgcc gccgcacccc ctctagcggg
     3361 cgcgggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt
     3421 cgccgcgccg ccgtcccctt ctccatctcc agcctcgggg ctgccgcagg gggacggctg
     3481 ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg gcggctctag
     3541 agcctctgct aaccatgttc atgccttctt ctttttccta cagatcctta attaaccgtc
     3601 ttaagcctgt aacaaccggt acagttcgaa taacttcgta tagcatacat tatacgaagt
     3661 tataagcttg catgcctgca ggtcggccgc cacgaccggc cggtgccgcc accatcccct
     3721 gacccacgcc cctgacccct cacaaggaga cgaccttcca tgaccgagta caagcccacg
     3781 gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc cgccgcgttc
     3841 gccgactacc ccgccacgcg ccacaccgtc gacccggacc gccacatcga gcgggtcacc
     3901 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     3961 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     4021 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     4081 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     4141 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     4201 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     4261 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     4321 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag
     4381 cgcccgaccg aaaggagcgc acgaccccat ggctccgacc gaagccaccc ggggcggccc
     4441 cgccgacccc gcacccgccc ccgaggccca ccgactctag aggatcataa tcagccatac
     4501 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     4561 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
     4621 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     4681 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccataact tcgtatagca
     4741 tacattatac gaagttatac cgggccacca tagtcgcgag tagcttgggc cagctaggcc
     4801 ttgaccaaag ttcctctgga attgcgatcg cacgcgtaaa tgattgcaga tccactagtt
     4861 ctagagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     4921 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     4981 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     5041 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     5101 gctctatggc ttctgaggcg gaaagaacca gctggggctc gagcctctag aactatagtg
     5161 agtcgtatta cgtagatcca gacatgataa gatacattga tgagtttgga caaaccacaa
     5221 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     5281 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
     5341 aggttcaggg ggaggtgtgg gaggtttttt aattcgcggc cctagaagat gggcgggagt
     5401 cttctgggca ggcttaaagg ctaacctggt gtgtgggcgt tgtcctgcag gggaattgaa
     5461 caggtgtaaa attggaggga caagacttcc cacagatttt cggttttgtc gggaagtttt
     5521 ttaatagggg caaataagga aaatgggagg ataggtagtc atctggggtt ttatgcagca
     5581 aaactacagg ttattattgc ttgtgatccg cctcggagta ttttccatcg aggtagatta
     5641 aagacatgct cacccgagtt ttatactctc ctgcttgaga tccttactac agtatgaaat
     5701 tacagtgtcg cgagttagac tatgtaagca gaattttaat catttttaaa gagcccagta
     5761 cttcatatcc atttctcccg ctccttctgc agccttatca aaaggtattt tagaacactc
     5821 attttagccc cattttcatt tattatactg gcttatccaa cccctagaca gagcattggc
     5881 attttccctt tcctgatctt agaagtctga tgactcatga aaccagacag attagttaca
     5941 tacaccacaa atcgaggctg tagctggggc ctcaacactg cagttctttt ataactcctt
     6001 agtacacttt ttgttgatcc tttgccttga tccttaattt tcagtgtcta tcacctctcc
     6061 cgtcaggtgg tgttccacat ttgggcctat tctcagtcca gggagtttta caacaataga
     6121 tgtattgaga atccaaccta aagcttaact ttccactccc atgaatgcct ctctcctttt
     6181 tctccattta taaactgagc tattaaccat taatggtttc caggtggatg tctcctcccc
     6241 caatattacc tgatgtatct tacatattgc caggctgata ttttaagaca ttaaaaggta
     6301 tatttcatta ttgagccaca tggtattgat tactgcttac taaaattttg tcattgtaca
     6361 catctgtaaa aggtggttcc ttttggaatg caaagttcag gtgtttgttg tctttcctga
     6421 cctaaggtct tgtgagcttg tattttttct atttaagcag tgctttctct tggactggct
     6481 tgactcatgg cattctacac gttattgctg gtctaaatgt gattttgcca agcttcttca
     6541 ggacctataa ttttgcttga cttgtagcca aacacaagta aaatgattaa gcaacaaatg
     6601 tatttgtgaa gcttggtttt taggttgttg tgttgtgtgt gcttgtgctc tataataata
     6661 ctatccaggg gctggagagg tggctcggag ttcaagagca cagactgctc ttccagaagt
     6721 cctgagttca attcccagca accacatggt ggctcacaac catctgtaat gggatctgat
     6781 gccctcttct ggtgtgtctg aagaccacaa gtgtattcac attaaataaa taaatcctcc
     6841 ttcttcttct tttttttttt tttaaagaga atactgtctc cagtagaatt tactgaagta
     6901 atgaaatact ttgtgtttgt tccaatatgg tagccaataa tcaaattact ctttaagcac
     6961 tggaaatgtt accaaggaac taatttttat ttgaagtgta actgtggaca gaggagccat
     7021 aactgcagac ttgtgggata cagaagacca atgcagactt taatgtcttt tctcttacac
     7081 taagcaataa agaaataaaa attgaacttc tagtatccta tttgtttaaa ctgctagctt
     7141 tacttaactt ttgtgcttca tctatacaaa gctgaaagct aagtctgcag ccattactaa
     7201 acatgaaagc aagtaatgat aattttggat ttcaaaaatg tagggccaga gtttagccag
     7261 ccagtggtgg tgcttgcctt tatgccttta atcccagcac tctggaggca gagacaggca
     7321 gatctctgag tttgagccca gcctggtcta cacatcaagt tctatctagg atagccagga
     7381 atacacacag aaaccctgtt ggggaggggg gctctgagat ttcataaaat tataattgaa
     7441 gcattcccta atgagccact atggatgtgg ctaaatccgt ctacctttct gatgagattt
     7501 gggtattatt ttttctgtct ctgctgttgg ttgggtcttt tgacactgtg ggctttcttt
     7561 aaagcctcct tcctgccatg tggtctcttg tttgctacta acttcccatg gcttaaatgg
     7621 catggctttt tgccttctaa gggcagctgc tgagatttgc agcctgattt ccagggtggg
     7681 gttgggaaat ctttcaaaca ctaaaattgt cctttaattt tttttttaaa aaatgggtta
     7741 tataataaac ctcataaaat agttatgagg agtgaggtgg actaatatta aatgagtccc
     7801 tcccctataa aagagctatt aaggcttttt gtcttatact taactttttt tttaaatgtg
     7861 gtatctttag aaccaagggt cttagagttt tagtatacag aaactgttgc atcgcttaat
     7921 cagattttct agtttcaaat ccagagaatc caaattcttc acagccaaag tcaaattaag
     7981 aatttctgac ttttaatgtt aatttgctta ctgtgaatat aaaaatgata gcttttcctg
     8041 aggcagggtc tcactatgta tctctgcctg atctgcaaca agatatgtag actaaagttc
     8101 tgcctgcttt tgtctcctga atactaaggt taaaatgtag taatactttt ggaacttgca
     8161 ggtcagattc ttttataggg gacacactaa gggagcttgg gtgatagttg gtaaaatgtg
     8221 tttcaagtga tgaaaacttg aattattatc accgcaacct actttttaaa aaaaaaagcc
     8281 aggcctgtta gagcatgctt aagggatccc taggacttgc tgagcacaca agagtagtta
     8341 cttggcaggc tcctggtgag agcatatttc aaaaaacaag gcagacaacc aagaaactac
     8401 agttaaggtt acctgtcttt aaaccatctg catatacaca gggatattaa aatattccaa
     8461 ataatatttc attcaagttt tcccccatca aattgggaca tggatttctc cggtgaatag
     8521 gcagagttgg aaactaaaca aatgttggtt ttgtgatttg tgaaattgtt ttcaagtgat
     8581 agttaaagcc catgagatac agaacaaagc tgctatttcg aggtctcttg gtttatactc
     8641 agaagcactt ctttgggttt ccctgcacta tcctgatcat gtgctaggcc taccttaggc
     8701 tgattgttgt tcaaataaac ttaagtttcc tgtcaggtga tgtcatatga tttcatatat
     8761 caaggcaaaa catgttatat atgttaaaca tttgtactta atgtgaaagt taggtctttg
     8821 tgggtttgat ttttaatttt caaaacctga gctaaataag tcatttttac atgtcttaca
     8881 tttggtggaa ttgtataatt gtggtttgca ggcaagactc tctgacctag taaccctacc
     8941 tatagagcac tttgctgggt cacaagtcta ggagtcaagc atttcacctt gaagttgaga
     9001 cgttttgtta gtgtatacta gtttatatgt tggaggacat gtttatccag aagatattca
     9061 ggactatttt tgactgggct aaggaattga ttctgattag cactgttagt gagcattgag
     9121 tggcctttag gcttgaattg gagtcacttg tatatctcaa ataatgctgg ccttttttaa
     9181 aaagcccttg ttctttatca ccctgttttc tacataattt ttgttcaaag aaatacttgt
     9241 ttggatctcc ttttgacaac aatagcatgt tttcaagcca tatttttttt cctttttttt
     9301 tttttttttg gtttttcgag acagggtttc tctgtatagc cctggctgtc ctggaactca
     9361 ctttgtagac caggctggcc tcgaactcag aaatccgcct gcctctgcct cctgagtgcc
     9421 gggattaaag gcgtgcacca ccacgcctgg ctaagttgga tattttgtta tataactata
     9481 accaatacta actccactgg gtggattttt aattcagtca gtagtcttaa gtggtcttta
     9541 ttggcccttc attaaaatct actgttcact ctaacagagg ctgttggtac tagtggcact
     9601 taagcaactt cctacggata tactagcaga ttaagggtca gggatagaaa ctagtctagc
     9661 gttttgtata cctaccagct ttatactacc ttgttctgat agaaatattt caggacatct
     9721 agcttatcga taccgtcgac ggtatcgata agcttgatcc agcttttgtt ccctttagtg
     9781 agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     9841 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     9901 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     9961 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
    10021 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
    10081 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
    10141 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
    10201 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
    10261 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
    10321 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
    10381 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
    10441 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
    10501 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
    10561 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
    10621 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
    10681 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
    10741 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
    10801 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
    10861 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
    10921 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
    10981 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
    11041 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
    11101 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
    11161 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
    11221 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
    11281 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
    11341 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
    11401 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
    11461 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
    11521 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
    11581 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    11641 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    11701 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    11761 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    11821 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    11881 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    11941 atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa attcgcgtta
    12001 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
    12061 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
    12121 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
    12181 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
    12241 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
    12301 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
    12361 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc
    12421 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
    12481 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
    12541 ttttcccagt cacgacgttg taaaacgacg gccagtgagc gcgcgtaata cgactcacta
    12601 tagggcgaat tggagct
//
