LOCUS       Exported               14805 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  recipient plasmid [multiplexing, nickase]; p35S:Cas9 D10A, BASTA.
ACCESSION   .
VERSION     .
KEYWORDS    pDGE77
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14805)
  AUTHORS   Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, 
            Boch J, Stuttmann J
  TITLE     Generation of chromosomal deletions in dicotyledonous plants 
            employing a user-friendly genome editing toolkit.
  JOURNAL   Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319.
  PUBMED    27579989
REFERENCE   2  (bases 1 to 14805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14805
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    302..326
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             1627..2256
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2690..3757
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      3823..4017
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(4253..4275)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    4361..4501
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4416..4435
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4516..4533)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4687..5275)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4767..4786)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5519..6310)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    6838..6862
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      6905..7157
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(7177..7728)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(7771..7950)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     terminator      8043..8295
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(8311..8331)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(8344..12447)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(12454..12474)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(12571..12916)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(12608..12628)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        13356..13458
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             13459..14118
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(13525..13544)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             14460..14765
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     14644..14663
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 ccatgctaga gtccgcaaaa atcaccagtc tctctctaca aatctatctc tctctatttt
       61 tctccagaat aatgtgtgag tagttcccag ataagggaat tagggttctt atagggtttc
      121 gctcatgtgt tgagcatata agaaaccctt agtatgtatt tgtatttgta aaatacttct
      181 atcaataaaa tttctaattc ctaaaaccaa aatccagtga cctgcaggca tgcgacgtcg
      241 agcttagctt gagcttggat cagattgtcg tttcccgcct tcagtttaaa ctatcagtgt
      301 ttgacaggat atattggcgg gtaaacctaa gagaaaagag cgtttattag aataatcgga
      361 tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac
      421 agggttcccc tcgggatcaa agtactttga tccaacccct ccgctgctat agtgcagtcg
      481 gcttctgacg ttcagtgcag ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta
      541 cgcgacaggc tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca
      601 taaagtagaa tacttgcgac tagaaccgga gacattacgc catgaacaag agcgccgccg
      661 ctggcctgct gggctatgcc cgcgtcagca ccgacgacca ggacttgacc aaccaacggg
      721 ccgaactgca cgcggccggc tgcaccaagc tgttttccga gaagatcacc ggcaccaggc
      781 gcgaccgccc ggagctggcc aggatgcttg accacctacg ccctggcgac gttgtgacag
      841 tgaccaggct agaccgcctg gcccgcagca cccgcgacct actggacatt gccgagcgca
      901 tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac accaccacgc
      961 cggccggccg catggtgttg accgtgttcg ccggcattgc cgagttcgag cgttccctaa
     1021 tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc
     1081 cccgccctac cctcaccccg gcacagatcg cgcacgcccg cgagctgatc gaccaggaag
     1141 gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg
     1201 cacttgagcg cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt gccttccgtg
     1261 aggacgcatt gaccgaggcc gacgccctgg cggccgccga gaatgaacgc caagaggaac
     1321 aagcatgaaa ccgcaccagg acggccagga cgaaccgttt ttcattaccg aagagatcga
     1381 ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg
     1441 gctgcatgaa atcctggccg gtttgtctga tgccaagctg gcggcctggc cggccagctt
     1501 ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag
     1561 cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta aataaacaaa tacgcaaggg
     1621 gaacgcatga aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc
     1681 gcaacccatc tagcccgcgc cctgcaactc gccggggccg atgttctgtt agtcgattcc
     1741 gatccccagg gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt
     1801 gtcggcatcg accgcccgac gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc
     1861 gtagtgatcg acggagcgcc ccaggcggcg gacttggctg tgtccgcgat caaggcagcc
     1921 gacttcgtgc tgattccggt gcagccaagc ccttacgaca tatgggccac cgccgacctg
     1981 gtggagctgg ttaagcagcg cattgaggtc acggatggaa ggctacaagc ggcctttgtc
     2041 gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg
     2101 tacgagctgc ccattcttga gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc
     2161 gccgccggca caaccgttct tgaatcagaa cccgagggcg acgctgcccg cgaggtccag
     2221 gcgctggccg ctgaaattaa atcaaaactc atttgagtta atgaggtaaa gagaaaatga
     2281 gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa
     2341 cgttggccag cctggcagac acgccagcca tgaagcgggt caactttcag ttgccggcgg
     2401 aggatcacac caagctgaag atgtacgcgg tacgccaagg caagaccatt accgagctgc
     2461 tatctgaata catcgcgcag ctaccagagt aaatgagcaa atgaataaat gagtagatga
     2521 attttagcgg ctaaaggagg cggcatggaa aatcaagaac aaccaggcac cgacgccgtg
     2581 gaatgcccca tgtgtggagg aacgggcggt tggccaggcg taagcggctg ggttgtctgc
     2641 cggccctgca atggcactgg aacccccaag cccgaggaat cggcgtgacg gtcgcaaacc
     2701 atccggcccg gtacaaatcg gcgcggcgct gggtgatgac ctggtggaga agttgaaggc
     2761 cgcgcaggcc gcccagcggc aacgcatcga ggcagaagca cgccccggtg aatcgtggca
     2821 agcggccgct gatcgaatcc gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc
     2881 gattaggaag ccgcccaagg gcgacgagca accagatttt ttcgttccga tgctctatga
     2941 cgtgggcacc cgcgatagtc gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg
     3001 tgaccgacga gctggcgagg tgatccgcta cgagcttcca gacgggcacg tagaggtttc
     3061 cgcagggccg gccggcatgg ccagtgtgtg ggattacgac ctggtactga tggcggtttc
     3121 ccatctaacc gaatccatga accgataccg ggaagggaag ggagacaagc ccggccgcgt
     3181 gttccgtcca cacgttgcgg acgtactcaa gttctgccgg cgagccgatg gcggaaagca
     3241 gaaagacgac ctggtagaaa cctgcattcg gttaaacacc acgcacgttg ccatgcagcg
     3301 tacgaagaag gccaagaacg gccgcctggt gacggtatcc gagggtgaag ccttgattag
     3361 ccgctacaag atcgtaaaga gcgaaaccgg gcggccggag tacatcgaga tcgagctagc
     3421 tgattggatg taccgcgaga tcacagaagg caagaacccg gacgtgctga cggttcaccc
     3481 cgattacttt ttgatcgatc ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc
     3541 cgcaggcaag gcagaagcca gatggttgtt caagacgatc tacgaacgca gtggcagcgc
     3601 cggagagttc aagaagttct gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc
     3661 ggagtacgat ttgaaggagg aggcggggca ggctggcccg atcctagtca tgcgctaccg
     3721 caacctgatc gagggcgaag catccgccgg ttcctaatgt acggagcaga tgctagggca
     3781 aattgcccta gcaggggaaa aaggtcgaaa aggtgtcttt cctgtggata gcacgtacat
     3841 tgggaaccca aagccgtaca ttgggaaccg gaacccgtac attgggaacc caaagccgta
     3901 cattgggaac cggtcacaca tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc
     3961 cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc cgcctggcct gtgcataact
     4021 gtctggccag cgcacagccg aagagctgca aaaagcgcct acccttcggt cgctgcgctc
     4081 cctacgcccc gccgcttcgc gtcggcctat cgcggccgct ggccgctcaa aaatggctgg
     4141 cctacggcca ggcaatctac cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg
     4201 gcgcccacat caaggcaccc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac
     4261 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     4321 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac
     4381 gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag
     4441 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
     4501 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     4561 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     4621 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     4681 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     4741 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     4801 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     4861 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     4921 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     4981 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     5041 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     5101 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     5161 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     5221 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     5281 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     5341 tttggtcatg catgatatat ctcccaattt gtgtagggct tattatgcac gcttaaaaat
     5401 aataaaagca gacttgacct gatagtttgg ctgtgagcaa ttatgtgctt agtgcatcta
     5461 atcgcttgag ttaacgccgg cgaagcggcg tcggcttgaa cgaatttcta gctagacatt
     5521 atttgccgac taccttggtg atctcgcctt tcacgtagtg gacaaattct tccaactgat
     5581 ctgcgcgcga ggccaagcga tcttcttctt gtccaagata agcctgtcta gcttcaagta
     5641 tgacgggctg atactgggcc ggcaggcgct ccattgccca gtcggcagcg acatccttcg
     5701 gcgcgatttt gccggttact gcgctgtacc aaatgcggga caacgtaagc actacatttc
     5761 gctcatcgcc agcccagtcg ggcggcgagt tccatagcgt taaggtttca tttagcgcct
     5821 caaatagatc ctgttcagga accggatcaa agagttcctc cgccgctgga cctaccaagg
     5881 caacgctatg ttctcttgct tttgtcagca agatagccag atcaatgtcg atcgtggctg
     5941 gctcgaagat acctgcaaga atgtcattgc gctgccattc tccaaattgc agttcgcgct
     6001 tagctggata acgccacgga atgatgtcgt cgtgcacaac aatggtgact tctacagcgc
     6061 ggagaatctc gctctctcca ggggaagccg aagtttccaa aaggtcgttg atcaaagctc
     6121 gccgcgttgt ttcatcaagc cttacggtca ccgtaaccag caaatcaata tcactgtgtg
     6181 gcttcaggcc gccatccact gcggagccgt acaaatgtac ggccagcaac gtcggttcga
     6241 gatggcgctc gatgacgcca actacctctg atagttgagt cgatacttcg gcgatcaccg
     6301 cttcccccat gatgtttaac tttgttttag ggcgactgcc ctgctgcgta acatcgttgc
     6361 tgctccataa catcaaacat cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag
     6421 gcatagactg taccccaaaa aaacatgtca taacaagaag ccatgaaaac cgccactgcg
     6481 ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgacgg cagttacgct
     6541 acttgcatta cagcttacga accgaacgag gcttatgtcc actgggttcg tgcccgaatt
     6601 gatcacaggc agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca
     6661 tccgtgtttc aaacccggca gcttagttgc cgttcttccg aatagcatcg gtaacatgag
     6721 caaagtctgc cgccttacaa cggctctccc gctgacgccg tcccggactg atgggctgcc
     6781 tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg agctgttggc tggctggtgg
     6841 caggatatat tgtggtgtaa acaaattgac gcttagacaa cttaataaca cattgcggaa
     6901 ttccgatcta gtaacataga tgacaccgcg cgcgataatt tatcctagtt tgcgcgctat
     6961 attttgtttt ctatcgcgta ttaaatgtat aattgcggga ctctaatcat aaaaacccat
     7021 ctcataaata acgtcatgca ttacatgtta attattacat gcttaacgta attcaacaga
     7081 aattatatga taatcatcgc aagaccggca acaggattca atcttaagaa actttattgc
     7141 caaatgtttg aacgatctgc ttgactctag gggtcatcag atttcggtga cgggcaggac
     7201 cggacggggc ggcaccggca ggctgaagtc cagctgccag aaacccacgt catgccagtt
     7261 cccgtgcttg aagccggccg cccgcagcat gccgcggggg gcatatccga gcgcctcgtg
     7321 catgcgcacg ctcgggtcgt tgggcagccc gatgacagcg accacgctct tgaagccctg
     7381 tgcctccagg gacttcagca ggtgggtgta gagcgtggag cccagtcccg tccgctggtg
     7441 gcggggggag acgtacacgg tcgactcggc cgtccagtcg taggcgttgc gtgccttcca
     7501 ggggcccgcg taggcgatgc cggcgacctc gccgtccacc tcggcgacga gccagggata
     7561 gcgctcccgc agacggacga ggtcgtccgt ccactcctgc ggttcctgcg gctcggtacg
     7621 gaagttgacc gtgcttgtct cgatgtagtg gttgacgatg gtgcagaccg ccggcatgtc
     7681 cgcctcggtg gcacggcgga tgtcggccgg gcgtcgttct gggctcatgg tagatccccc
     7741 tcgatcgagt tgagagtgaa tatgagactc taattggata ccgaggggaa tttatggaac
     7801 gtcagtggag catttttgac aagaaatatt tgctagctga tagtgacctt aggcgacttt
     7861 tgaacgcgca ataatggttt ctgacgtatg tgcttagctc attaaactcc agaaacccgc
     7921 ggctgagtgg ctccttcaac gttgcggttc tgtcagttcc aaacgtaaaa cggcttgtcc
     7981 cgcgtcatcg gcgggggtca taacgtgact cccttaattc tccgctcatg attagtagcg
     8041 tcgatctagt aacatagatg acaccgcgcg cgataattta tcctagtttg cgcgctatat
     8101 tttgttttct atcgcgtatt aaatgtataa ttgcgggact ctaatcataa aaacccatct
     8161 cataaataac gtcatgcatt acatgttaat tattacatgc ttaacgtaat tcaacagaaa
     8221 ttatatgata atcatcgcaa gaccggcaac aggattcaat cttaagaaac tttattgcca
     8281 aatgtttgaa cgatctgctt gacaagctca caccttcctc ttcttcttgg ggtcagccct
     8341 gctgtctcca ccgagctgag agaggtcgat tcttgtttca tagagccccg taattgactg
     8401 atgaatcagt gtggcgtcca ggacctcctt tgtagaggtg taccgctttc tgtctatggt
     8461 ggtgtcgaag tacttgaagg ctgcaggcgc gcccaagttg gtcagagtaa acaagtggat
     8521 aatgttttct gcctgctccc tgatgggctt atccctgtgc ttattgtaag cagaaagcac
     8581 cttatcgagg ttagcgtcgg cgaggatcac tcttttggag aattcgctta tttgctcgat
     8641 gatctcatca aggtagtgtt tgtgttgttc cacgaacagc tgcttctgct cattatcttc
     8701 gggagatcct ttgagctttt catagtggct ggccagatac aagaaattaa cgtatttaga
     8761 gggcagtgcc agctcgttac ctttctgcag ctcgcccgca ctagcgagca ttcgtttccg
     8821 gccgttttca agctcaaaga gagagtactt gggaagctta atgatgaggt cttttttgac
     8881 ctctttatat cctttcgcct cgagaaagtc gatggggttt ttttcgaagc ttgatcgctc
     8941 catgattgtg atgcccagca gttccttgac gcttttgagt tttttagact tccctttctc
     9001 cactttggcc acaaccagta cactgtaagc gactgtagga gaatcgaatc cgccgtattt
     9061 cttggggtcc caatcttttt tgcgtgcgat cagcttgtcg ctgttccttt tcgggaggat
     9121 actttccttg gagaagcctc cggtctgtac ttcggtcttt ttaacgatgt tcacctgcgg
     9181 catggacagg accttccgga ctgtcgcgaa atccctaccc ttgtcccaca cgatttctcc
     9241 tgtttctccg tttgtttcga taagtggtcg cttccgaatc tctccattgg ccagtgtaat
     9301 ctcggtcttg aaaaaattca taatattgct gtaaaagaag tacttagcgg tggccttgcc
     9361 tatttcctgc tcagactttg cgatcatttt cctaacatcg tacactttat agtctccgta
     9421 aacaaattca gattcaagct tgggatattt tttgataagt gcagtgccta ccactgcatt
     9481 caggtaggca tcatgcgcat ggtggtaatt gttgatctct ctcaccttat aaaactgaaa
     9541 gtcctttctg aaatctgaaa ccagcttaga cttcagagta ataactttca cctctcgaat
     9601 cagtttgtca ttttcatcgt acttggtgtt catgcgtgaa tcgagaattt gggccacgtg
     9661 cttggtgatc tggcgtgtct caacaagctg ccttttgatg aagccggctt tatccaactc
     9721 agacaggcca cctcgttcag ccttagtcag attatcgaac ttccgttgtg tgatcagttt
     9781 ggcgttcagc agctgccgcc aataattttt cattttcttg acaacttctt ctgaggggac
     9841 gttatcactc ttccctctat ttttatcgga tcttgtcaac actttattat caatagaatc
     9901 atctttgaga aaagactggg gcacgatatg atccacgtcg tagtcggaga gccgattgat
     9961 gtccagttcc tgatccacgt acatgtccct gccgttctgc aggtagtaca ggtagagctt
    10021 ctcattctga agctgggtgt tttcaactgg gtgttcctta aggatttggg accccagttc
    10081 ttttataccc tcttcaatcc tcttcatcct ttccctactg ttcttctgtc ccttctgggt
    10141 agtttggttc tctcgggcca tctcgataac gatattctcg ggcttatgcc ttcccattac
    10201 tttgacgagt tcatccacga ccttaacggt ctgcagtatt ccctttttga tagctgggct
    10261 acctgcaaga ttagcgatgt gctcgtggag actgtccccc tggccagaaa cttgtgcttt
    10321 ctggatgtcc tccttaaagg tgagagagtc atcatggatc aactgcatga agttccggtt
    10381 ggcaaatcca tcggacttaa gaaaatccag gattgtcttt ccactctgct tgtctcggat
    10441 cccattgatc agttttcttg acagccgccc ccatcctgta tatcggcgcc tcttgagctg
    10501 tttcatgact ttgtcgtcga agagatgagc gtaagttttc aagcgttctt caatcatctc
    10561 cctatcttca aacaacgtaa gggtgaggac aatgtcctca agaatgtcct cgttctcctc
    10621 attgtccagg aagtccttgt ctttaatgat tttcaggaga tcgtgatacg ttcccaggga
    10681 tgcgttgaag cgatcctcca ctccgctgat ttcaacagag tcgaaacatt caatcttttt
    10741 gaaataatct tctttgagct gtttcacggt aactttccgg ttcgtcttga agaggaggtc
    10801 cacgatagct ttcttctgct ctccagacag gaatgctggc tttctcatcc cttctgtgac
    10861 gtatttgacc ttggtgagct cgttataaac tgtgaagtac tcgtacagca gagagtgttt
    10921 aggaagcacc ttttcgttag gcagattttt atcaaagtta gtcatccttt cgatgaagga
    10981 ctgggcagag gcccccttat ccacgacttc ctcgaagttc cagggagtga tagtctcttc
    11041 tgatttgcga gtcatccacg cgaatctgga atttccccgg gcgagggggc ctacatagta
    11101 gggtatccga aatgtgagga ttttctcaat cttttccctg ttatctttca aaaaggggta
    11161 gaaatcctct tgccgcctga ggatagcgtg cagttcgccc aggtgaatct ggtgggggat
    11221 gcttccattg tcgaaagtgc gctgtttgcg caacagatct tctctgttaa gctttaccag
    11281 cagctcctcg gtgccgtcca ttttttccaa gatgggctta ataaatttgt aaaattcctc
    11341 ctggcttgct ccgccgtcaa tgtatccggc gtagccattt ttagactgat cgaagaaaat
    11401 ttccttgtac ttctcaggca gttgctgtct gacaagggcc ttcagcaaag tcaagtcttg
    11461 gtggtgctca tcatagcgct tgatcatact agcgctcagc ggagctttgg tgatctccgt
    11521 gttcactcgc agaatatcac tcagcagaat ggcgtctgac aggttctttg ccgccaaaaa
    11581 aaggtctgcg tactggtcgc cgatctgggc cagcagattg tcgagatcat catcgtaggt
    11641 gtctttgctc agttgaagct tggcatcttc ggccaggtcg aagttagatt taaagttggg
    11701 ggtcagcccg agtgacaggg cgataagatt accaaacagg ccgttcttct tctccccagg
    11761 gagctgtgcg atgaggtttt cgagccgccg ggatttggac agcctagcgc tcaggattgc
    11821 tttggcgtca actccggatg cgttgatcgg gttctcttcg aaaagctgat tgtaagtctg
    11881 aaccagttgg ataaagagtt tgtcgacatc gctgttgtct gggttcaggt ccccctcgat
    11941 gaggaagtgt ccccgaaatt tgatcatatg cgccagcgcg agatagatca accgcaagtc
    12001 agccttatca gtactgtcta caagcttctt cctcagatga tatatggttg ggtacttttc
    12061 atggtacgcc acctcgtcca cgatattgcc aaagattggg tggcgctcgt gctttttatc
    12121 ctcctccacc aaaaaggact cctccagcct atggaagaaa gagtcatcca ccttagccat
    12181 ctcattacta aagatctcct gcaggtagca gatccgattc tttctgcggg tatatctgcg
    12241 ccgtgctgtt cttttgagcc gcgtggcttc ggccgtctcc ccggagtcga acaggagggc
    12301 gccaatgagg ttcttcttta tgctgtggcg atcggtattg cccagaactt tgaatttttt
    12361 gctcggcacc ttgtactcgt ccgtaatgac ggcccagccg acgctgtttg tgccgatagc
    12421 gagcccaatg gagtacttct tgtccatgcc accaaccttc cgcttcttct taggagccat
    12481 tgtatcgata attgtaaatg taattgtaat gttgtttgtt gtttgttgtt gttggtaatt
    12541 gttgtaaaaa tgagctctta tactcgagcg tgtcctctcc aaatgaaatg aacttcctta
    12601 tatagaggaa gggtcttgcg aaggatagtg ggattgtgcg tcatccctta cgtcagtgga
    12661 gatgtcacat caatccactt gctttgtaga cgtggttgga acctcttctt tttccacgat
    12721 gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagagagat cttgaatgat
    12781 agcctttcct ttatcgcaat gatggcattt gtaggagcca ccttcctttt ctactgtcct
    12841 ttcgatgaag tgacagatag ctgggcaatg gaatccgagg aggtttcccg aaattatcct
    12901 ttgttgaaaa gtctcaatag ccctttgatc ttctgagact gtatctttga catttttgga
    12961 gtagaccaga gtgtcgtgct ccaccatgtt atcacatcaa tccacttgct ttgtagacgt
    13021 ggttggaacc tcttcttttt ccacgatgct cctcgtgggt gggggtccat ctttgggacc
    13081 actgtcggca gagagatctt gaatgatagc ctttccttta tcgcaatgat ggcatttgta
    13141 ggagccacct tccttttcta ctgtcctttc gatgaagtga cagatagctg ggcaatggaa
    13201 tccgaggagg tttcccgaaa ttatcctttg ttgaaaagtc tcaatagccc tttgatcttc
    13261 tgagactgta tctttgacat ttttggagta gaccagagtg tcgtgctcca ccatgttgac
    13321 ctccaagctt gaattcttgc agagacctga gacgttgatc ggcacgtaag aggttccaac
    13381 tttcaccata atgaaataag atcactaccg ggcgtatttt ttgagttatc gagattttca
    13441 ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc
    13501 caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac
    13561 cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag
    13621 ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc ggaattccgt
    13681 atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt
    13741 ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg
    13801 cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc
    13861 cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt gagtttcacc
    13921 agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc
    13981 aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc
    14041 gtctgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag
    14101 tggcagggcg gggcgtaatc gcgtggatcc ggcttactaa aagccagata acagtatgcg
    14161 tatttgcgcg ctgatttttg cggtataaga atatatactg atatgtatac ccgaagtatg
    14221 tcaaaaagag gtatgctatg aagcagcgta ttacagtgac agttgacagc gacagctatc
    14281 agttgctcaa ggcatatatg atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa
    14341 tgaagcccgt cgtctgcgtg ccgaacgctg gaaagcggaa aatcaggaag ggatggctga
    14401 ggtcgcccgg tttattgaaa tgaacggctc ttttgctgac gagaacaggg gctggtgaaa
    14461 tgcagtttaa ggtttacacc tataaaagag agagccgtta tcgtctgttt gtggatgtac
    14521 agagtgatat tattgacacg cccgggcgac ggatggtgat ccccctggcc agtgcacgtc
    14581 tgctgtcaga taaagtctcc cgtgaacttt acccggtggt gcatatcggg gatgaaagct
    14641 ggcgcatgat gaccaccgat atggccagtg tgccggtttc cgttatcggg gaagaagtgg
    14701 ctgatctcag ccaccgcgaa aatgacatca aaaacgccat taacctgatg ttctggggaa
    14761 tataaatgtc aggctccctt atacacagcc agtcggtctc agctt
//
