LOCUS Exported 15284 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION recipient plasmid [multiplexing, activator]; p35S:Cas9 D10A/N863A-Hax3CT, BASTA. ACCESSION . VERSION . KEYWORDS pJOG251 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 15284) AUTHORS Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, Boch J, Stuttmann J TITLE Generation of chromosomal deletions in dicotyledonous plants employing a user-friendly genome editing toolkit. JOURNAL Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319. PUBMED 27579989 REFERENCE 2 (bases 1 to 15284) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..15284 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 266..290 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA" terminator 333..585 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" CDS complement(605..1156) /codon_start=1 /gene="Streptomyces hygroscopicus bar" /product="phosphinothricin acetyltransferase" /label=BlpR /note="confers resistance to bialophos or phosphinothricin" /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF WQLDFSLPVPPRPVLPVTEI" promoter complement(1199..1378) /label=NOS promoter /note="nopaline synthase promoter" polyA_signal 1468..1642 /label=CaMV poly(A) signal /note="cauliflower mosaic virus polyadenylation signal" CDS complement(2545..2565) /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS complement(2578..6681) /codon_start=1 /product="catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=dCas9 /note="RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the N863A mutation in the HNH catalytic domain" /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKARGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" CDS complement(6688..6708) /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" promoter complement(6805..7150) /label=CaMV 35S promoter /note="strong constitutive promoter from cauliflower mosaic virus" primer_bind complement(6842..6862) /label=35S promoter /note="CaMV 35S promoter, forward primer" promoter 7263..7365 /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" CDS 7366..8025 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind complement(7432..7451) /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" CDS 8367..8672 /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /label=ccdB /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" primer_bind 8551..8570 /label=ccdB-fwd /note="ccdB gene, forward primer" misc_feature 9014..9038 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" CDS 10339..10968 /codon_start=1 /product="stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 StaA /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI" CDS 11402..12469 /codon_start=1 /product="replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 RepA /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV MRYRNLIEGEASAGS" rep_origin 12535..12729 /label=pVS1 oriV /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" primer_bind complement(12965..12987) /label=pGEX 3' /note="pGEX vectors, reverse primer" misc_feature 13073..13213 /label=bom /note="basis of mobility region from pBR322" primer_bind 13128..13147 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind complement(13228..13245) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(13399..13987) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(13479..13498) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(14231..15022) /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" ORIGIN 1 ttatgtccac tgggttcgtg cccgaattga tcacaggcag caacgctctg tcatcgttac 61 aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa acccggcagc ttagttgccg 121 ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg gctctcccgc 181 tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc 241 ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac aaattgacgc 301 ttagacaact taataacaca ttgcggaatt ccgatctagt aacatagatg acaccgcgcg 361 cgataattta tcctagtttg cgcgctatat tttgttttct atcgcgtatt aaatgtataa 421 ttgcgggact ctaatcataa aaacccatct cataaataac gtcatgcatt acatgttaat 481 tattacatgc ttaacgtaat tcaacagaaa ttatatgata atcatcgcaa gaccggcaac 541 aggattcaat cttaagaaac tttattgcca aatgtttgaa cgatctgctt gactctaggg 601 gtcatcagat ttcggtgacg ggcaggaccg gacggggcgg caccggcagg ctgaagtcca 661 gctgccagaa acccacgtca tgccagttcc cgtgcttgaa gccggccgcc cgcagcatgc 721 cgcggggggc atatccgagc gcctcgtgca tgcgcacgct cgggtcgttg ggcagcccga 781 tgacagcgac cacgctcttg aagccctgtg cctccaggga cttcagcagg tgggtgtaga 841 gcgtggagcc cagtcccgtc cgctggtggc ggggggagac gtacacggtc gactcggccg 901 tccagtcgta ggcgttgcgt gccttccagg ggcccgcgta ggcgatgccg gcgacctcgc 961 cgtccacctc ggcgacgagc cagggatagc gctcccgcag acggacgagg tcgtccgtcc 1021 actcctgcgg ttcctgcggc tcggtacgga agttgaccgt gcttgtctcg atgtagtggt 1081 tgacgatggt gcagaccgcc ggcatgtccg cctcggtggc acggcggatg tcggccgggc 1141 gtcgttctgg gctcatggta gatccccctc gatcgagttg agagtgaata tgagactcta 1201 attggatacc gaggggaatt tatggaacgt cagtggagca tttttgacaa gaaatatttg 1261 ctagctgata gtgaccttag gcgacttttg aacgcgcaat aatggtttct gacgtatgtg 1321 cttagctcat taaactccag aaacccgcgg ctgagtggct ccttcaacgt tgcggttctg 1381 tcagttccaa acgtaaaacg gcttgtcccg cgtcatcggc gggggtcata acgtgactcc 1441 cttaattctc cgctcatgat tagtagcgat ctggatttta gtactggatt ttggttttag 1501 gaattagaaa ttttattgat agaagtattt tacaaataca aatacatact aagggtttct 1561 tatatgctca acacatgagc gaaaccctat aggaacccta attcccttat ctgggaacta 1621 ctcacacatt attatggaga aactcgagct tgtcgatcga ctctagctag agaagcttca 1681 ctgaggcaat agctccatca accatgcgag ctcctcttcg ttgaatgccg ggaaatcatc 1741 cgctgtccct gcgaaggggt ccgcatcttg ttcccaaacc acggtgctgg acgctaccag 1801 gtcggcatcc atgggcgtac caggatccag gaggccgccg atcctggtac gcgggcgttt 1861 tacaccccag ctgaggaggg gcaaatgcaa cgcatcgcgc tgttcgggaa cgcgcacctc 1921 gaacgattgc tgtgcggagg gaccggtgac agcacgatcc gatcgggacc gtttacggct 1981 gcttgcccgc gtctgatctc cctcgtgcat tgggctaggc gcatcaaggt cacgctccag 2041 cgaatcggcg aatgcatgca aagacgcctg atccggcgtt tgagttgaag taggggacgg 2101 tttggccctt ttcatccctg atgcctggag gatacggtcc caacgctgcg aggctggggg 2161 gagcgttcca ctgcgggctt cgagttcggt gacgcccact ctgcgaaaga gctgtaacaa 2221 cccgtgcctg ctcatcccga actgcgtcat ggcgtcatca aatgcttgcg ctgggtggga 2281 gtggcactgg aaaaaaccca gcacgcgaac cacttgcgcg tggtcggcaa cgcgatggga 2341 tgtgcgttcg ggaatacggc gattggttct tttgatcaag gccggcgcgt gcggcaatcc 2401 ctttttcact gcatccagcg caggacgtcc gccgaggcag gccaaggcga cgaggtggtc 2461 gttggtcaac gcggccaacg ccggatcagg gcgagataac tgggcaacaa tgcttccacc 2521 gccaccacta ccgccacccg aacccacctt cctcttcttc ttggggtcag ccctgctgtc 2581 tccaccgagc tgagagaggt cgattcttgt ttcatagagc cccgtaattg actgatgaat 2641 cagtgtggcg tccaggacct cctttgtaga ggtgtaccgc tttctgtcta tggtggtgtc 2701 gaagtacttg aaggctgcag gcgcgcccaa gttggtcaga gtaaacaagt ggataatgtt 2761 ttctgcctgc tccctgatgg gcttatccct gtgcttattg taagcagaaa gcaccttatc 2821 gaggttagcg tcggcgagga tcactctttt ggagaattcg cttatttgct cgatgatctc 2881 atcaaggtag tgtttgtgtt gttccacgaa cagctgcttc tgctcattat cttcgggaga 2941 tcctttgagc ttttcatagt ggctggccag atacaagaaa ttaacgtatt tagagggcag 3001 tgccagctcg ttacctttct gcagctcgcc cgcactagcg agcattcgtt tccggccgtt 3061 ttcaagctca aagagagagt acttgggaag cttaatgatg aggtcttttt tgacctcttt 3121 atatcctttc gcctcgagaa agtcgatggg gtttttttcg aagcttgatc gctccatgat 3181 tgtgatgccc agcagttcct tgacgctttt gagtttttta gacttccctt tctccacttt 3241 ggccacaacc agtacactgt aagcgactgt aggagaatcg aatccgccgt atttcttggg 3301 gtcccaatct tttttgcgtg cgatcagctt gtcgctgttc cttttcggga ggatactttc 3361 cttggagaag cctccggtct gtacttcggt ctttttaacg atgttcacct gcggcatgga 3421 caggaccttc cggactgtcg cgaaatccct acccttgtcc cacacgattt ctcctgtttc 3481 tccgtttgtt tcgataagtg gtcgcttccg aatctctcca ttggccagtg taatctcggt 3541 cttgaaaaaa ttcataatat tgctgtaaaa gaagtactta gcggtggcct tgcctatttc 3601 ctgctcagac tttgcgatca ttttcctaac atcgtacact ttatagtctc cgtaaacaaa 3661 ttcagattca agcttgggat attttttgat aagtgcagtg cctaccactg cattcaggta 3721 ggcatcatgc gcatggtggt aattgttgat ctctctcacc ttataaaact gaaagtcctt 3781 tctgaaatct gaaaccagct tagacttcag agtaataact ttcacctctc gaatcagttt 3841 gtcattttca tcgtacttgg tgttcatgcg tgaatcgaga atttgggcca cgtgcttggt 3901 gatctggcgt gtctcaacaa gctgcctttt gatgaagccg gctttatcca actcagacag 3961 gccacctcgt tcagccttag tcagattatc gaacttccgt tgtgtgatca gtttggcgtt 4021 cagcagctgc cgccaataat ttttcatttt cttgacaact tcttctgagg ggacgttatc 4081 actcttccct ctagctttat cggatcttgt caacacttta ttatcaatag aatcatcttt 4141 gagaaaagac tggggcacga tatgatccac gtcgtagtcg gagagccgat tgatgtccag 4201 ttcctgatcc acgtacatgt ccctgccgtt ctgcaggtag tacaggtaga gcttctcatt 4261 ctgaagctgg gtgttttcaa ctgggtgttc cttaaggatt tgggacccca gttcttttat 4321 accctcttca atcctcttca tcctttccct actgttcttc tgtcccttct gggtagtttg 4381 gttctctcgg gccatctcga taacgatatt ctcgggctta tgccttccca ttactttgac 4441 gagttcatcc acgaccttaa cggtctgcag tattcccttt ttgatagctg ggctacctgc 4501 aagattagcg atgtgctcgt ggagactgtc cccctggcca gaaacttgtg ctttctggat 4561 gtcctcctta aaggtgagag agtcatcatg gatcaactgc atgaagttcc ggttggcaaa 4621 tccatcggac ttaagaaaat ccaggattgt ctttccactc tgcttgtctc ggatcccatt 4681 gatcagtttt cttgacagcc gcccccatcc tgtatatcgg cgcctcttga gctgtttcat 4741 gactttgtcg tcgaagagat gagcgtaagt tttcaagcgt tcttcaatca tctccctatc 4801 ttcaaacaac gtaagggtga ggacaatgtc ctcaagaatg tcctcgttct cctcattgtc 4861 caggaagtcc ttgtctttaa tgattttcag gagatcgtga tacgttccca gggatgcgtt 4921 gaagcgatcc tccactccgc tgatttcaac agagtcgaaa cattcaatct ttttgaaata 4981 atcttctttg agctgtttca cggtaacttt ccggttcgtc ttgaagagga ggtccacgat 5041 agctttcttc tgctctccag acaggaatgc tggctttctc atcccttctg tgacgtattt 5101 gaccttggtg agctcgttat aaactgtgaa gtactcgtac agcagagagt gtttaggaag 5161 caccttttcg ttaggcagat ttttatcaaa gttagtcatc ctttcgatga aggactgggc 5221 agaggccccc ttatccacga cttcctcgaa gttccaggga gtgatagtct cttctgattt 5281 gcgagtcatc cacgcgaatc tggaatttcc ccgggcgagg gggcctacat agtagggtat 5341 ccgaaatgtg aggattttct caatcttttc cctgttatct ttcaaaaagg ggtagaaatc 5401 ctcttgccgc ctgaggatag cgtgcagttc gcccaggtga atctggtggg ggatgcttcc 5461 attgtcgaaa gtgcgctgtt tgcgcaacag atcttctctg ttaagcttta ccagcagctc 5521 ctcggtgccg tccatttttt ccaagatggg cttaataaat ttgtaaaatt cctcctggct 5581 tgctccgccg tcaatgtatc cggcgtagcc atttttagac tgatcgaaga aaatttcctt 5641 gtacttctca ggcagttgct gtctgacaag ggccttcagc aaagtcaagt cttggtggtg 5701 ctcatcatag cgcttgatca tactagcgct cagcggagct ttggtgatct ccgtgttcac 5761 tcgcagaata tcactcagca gaatggcgtc tgacaggttc tttgccgcca aaaaaaggtc 5821 tgcgtactgg tcgccgatct gggccagcag attgtcgaga tcatcatcgt aggtgtcttt 5881 gctcagttga agcttggcat cttcggccag gtcgaagtta gatttaaagt tgggggtcag 5941 cccgagtgac agggcgataa gattaccaaa caggccgttc ttcttctccc cagggagctg 6001 tgcgatgagg ttttcgagcc gccgggattt ggacagccta gcgctcagga ttgctttggc 6061 gtcaactccg gatgcgttga tcgggttctc ttcgaaaagc tgattgtaag tctgaaccag 6121 ttggataaag agtttgtcga catcgctgtt gtctgggttc aggtccccct cgatgaggaa 6181 gtgtccccga aatttgatca tatgcgccag cgcgagatag atcaaccgca agtcagcctt 6241 atcagtactg tctacaagct tcttcctcag atgatatatg gttgggtact tttcatggta 6301 cgccacctcg tccacgatat tgccaaagat tgggtggcgc tcgtgctttt tatcctcctc 6361 caccaaaaag gactcctcca gcctatggaa gaaagagtca tccaccttag ccatctcatt 6421 actaaagatc tcctgcaggt agcagatccg attctttctg cgggtatatc tgcgccgtgc 6481 tgttcttttg agccgcgtgg cttcggccgt ctccccggag tcgaacagga gggcgccaat 6541 gaggttcttc tttatgctgt ggcgatcggt attgcccaga actttgaatt ttttgctcgg 6601 caccttgtac tcgtccgtaa tgacggccca gccgacgctg tttgtgccga tagcgagccc 6661 aatggagtac ttcttgtcca tgccaccaac cttccgcttc ttcttaggag ccattgtatc 6721 gataattgta aatgtaattg taatgttgtt tgttgtttgt tgttgttggt aattgttgta 6781 aaaatgagct cttatactcg agcgtgtcct ctccaaatga aatgaacttc cttatataga 6841 ggaagggtct tgcgaaggat agtgggattg tgcgtcatcc cttacgtcag tggagatgtc 6901 acatcaatcc acttgctttg tagacgtggt tggaacctct tctttttcca cgatgctcct 6961 cgtgggtggg ggtccatctt tgggaccact gtcggcagag agatcttgaa tgatagcctt 7021 tcctttatcg caatgatggc atttgtagga gccaccttcc ttttctactg tcctttcgat 7081 gaagtgacag atagctgggc aatggaatcc gaggaggttt cccgaaatta tcctttgttg 7141 aaaagtctca atagcccttt gatcttctga gactgtatct ttgacatttt tggagtagac 7201 cagagtgtcg tgctccacca tgttgacctc caagcttgaa ttcttgcaga gacctgagac 7261 gttgatcggc acgtaagagg ttccaacttt caccataatg aaataagatc actaccgggc 7321 gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc 7381 actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt 7441 cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta 7501 aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc 7561 ctgatgaatg ctcatccgga attccgtatg gcaatgaaag acggtgagct ggtgatatgg 7621 gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc 7681 tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg 7741 tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc 7801 tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac 7861 ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg 7921 ccgctggcga ttcaggttca tcatgccgtc tgtgatggct tccatgtcgg cagaatgctt 7981 aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaatcgcg tggatccggc 8041 ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg tataagaata 8101 tatactgata tgtatacccg aagtatgtca aaaagaggta tgctatgaag cagcgtatta 8161 cagtgacagt tgacagcgac agctatcagt tgctcaaggc atatatgatg tcaatatctc 8221 cggtctggta agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa 8281 agcggaaaat caggaaggga tggctgaggt cgcccggttt attgaaatga acggctcttt 8341 tgctgacgag aacaggggct ggtgaaatgc agtttaaggt ttacacctat aaaagagaga 8401 gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccc gggcgacgga 8461 tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtctcccgt gaactttacc 8521 cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg gccagtgtgc 8581 cggtttccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa 8641 acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata cacagccagt 8701 cggtctcagc ttccatgcta gagtccgcaa aaatcaccag tctctctcta caaatctatc 8761 tctctctatt tttctccaga ataatgtgtg agtagttccc agataaggga attagggttc 8821 ttatagggtt tcgctcatgt gttgagcata taagaaaccc ttagtatgta tttgtatttg 8881 taaaatactt ctatcaataa aatttctaat tcctaaaacc aaaatccagt gacctgcagg 8941 catgcgacgt cgagcttagc ttgagcttgg atcagattgt cgtttcccgc cttcagttta 9001 aactatcagt gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt 9061 agaataatcg gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg 9121 catgccaacc acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct 9181 atagtgcagt cggcttctga cgttcagtgc agccgtgttc tgaaaacgac atgtcgcaca 9241 agtcctaagt tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt 9301 gttttagtcg cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca 9361 agagcgccgc cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga 9421 ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca 9481 ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg 9541 acgttgtgac agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca 9601 ttgccgagcg catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg 9661 acaccaccac gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg 9721 agcgttccct aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg 9781 tgaagtttgg cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga 9841 tcgaccagga aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga 9901 ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg 9961 gtgccttccg tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac 10021 gccaagagga acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac 10081 cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt 10141 ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg 10201 gccggccagc ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt 10261 tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca 10321 aatacgcaag gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc 10381 aagacgacca tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg 10441 ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa 10501 ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc 10561 cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg 10621 atcaaggcag ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc 10681 accgccgacc tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa 10741 gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag 10801 gcgctggccg ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac 10861 ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc 10921 cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta 10981 aagagaaaat gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca 11041 gcaaggctgc aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc 11101 agttgccggc ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca 11161 ttaccgagct gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa 11221 atgagtagat gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc 11281 accgacgccg tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc 11341 tgggttgtct gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga 11401 cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga 11461 gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg 11521 tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc 11581 cggtgcgccg tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc 11641 gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg 11701 tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca 11761 cgtagaggtt tccgcagggc cggccggcat ggccagtgtg tgggattacg acctggtact 11821 gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa 11881 gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga 11941 tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt 12001 tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga 12061 agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga 12121 gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct 12181 gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct 12241 ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg 12301 cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc 12361 aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt 12421 catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat gtacggagca 12481 gatgctaggg caaattgccc tagcagggga aaaaggtcga aaaggtgtct ttcctgtgga 12541 tagcacgtac attgggaacc caaagccgta cattgggaac cggaacccgt acattgggaa 12601 cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa aagagaaaaa 12661 aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa cccgcctggc 12721 ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc ctacccttcg 12781 gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc 12841 aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg cgccgtcgcc 12901 actcgaccgc cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg 12961 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg 13021 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca 13081 tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca 13141 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa 13201 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 13261 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 13321 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 13381 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 13441 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 13501 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 13561 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 13621 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 13681 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 13741 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 13801 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc 13861 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 13921 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 13981 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 14041 acgttaaggg attttggtca tgcatgatat atctcccaat ttgtgtaggg cttattatgc 14101 acgcttaaaa ataataaaag cagacttgac ctgatagttt ggctgtgagc aattatgtgc 14161 ttagtgcatc taatcgcttg agttaacgcc ggcgaagcgg cgtcggcttg aacgaatttc 14221 tagctagaca ttatttgccg actaccttgg tgatctcgcc tttcacgtag tggacaaatt 14281 cttccaactg atctgcgcgc gaggccaagc gatcttcttc ttgtccaaga taagcctgtc 14341 tagcttcaag tatgacgggc tgatactggg ccggcaggcg ctccattgcc cagtcggcag 14401 cgacatcctt cggcgcgatt ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa 14461 gcactacatt tcgctcatcg ccagcccagt cgggcggcga gttccatagc gttaaggttt 14521 catttagcgc ctcaaataga tcctgttcag gaaccggatc aaagagttcc tccgccgctg 14581 gacctaccaa ggcaacgcta tgttctcttg cttttgtcag caagatagcc agatcaatgt 14641 cgatcgtggc tggctcgaag atacctgcaa gaatgtcatt gcgctgccat tctccaaatt 14701 gcagttcgcg cttagctgga taacgccacg gaatgatgtc gtcgtgcaca acaatggtga 14761 cttctacagc gcggagaatc tcgctctctc caggggaagc cgaagtttcc aaaaggtcgt 14821 tgatcaaagc tcgccgcgtt gtttcatcaa gccttacggt caccgtaacc agcaaatcaa 14881 tatcactgtg tggcttcagg ccgccatcca ctgcggagcc gtacaaatgt acggccagca 14941 acgtcggttc gagatggcgc tcgatgacgc caactacctc tgatagttga gtcgatactt 15001 cggcgatcac cgcttccccc atgatgttta actttgtttt agggcgactg ccctgctgcg 15061 taacatcgtt gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct 15121 tggatgcccg aggcatagac tgtaccccaa aaaaacatgt cataacaaga agccatgaaa 15181 accgccactg cgccgttacc accgctgcgt tcggtcaagg ttctggacca gttgcgtgac 15241 ggcagttacg ctacttgcat tacagcttac gaaccgaacg aggc //