LOCUS       Exported               15464 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  recipient plasmid [single activator]; p35S:Cas9 D10A/N863A-Hax3CT, 
            BASTA.
ACCESSION   .
VERSION     .
KEYWORDS    pJOG253
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15464)
  AUTHORS   Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, 
            Boch J, Stuttmann J
  TITLE     Generation of chromosomal deletions in dicotyledonous plants 
            employing a user-friendly genome editing toolkit.
  JOURNAL   Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319.
  PUBMED    27579989
REFERENCE   2  (bases 1 to 15464)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..15464
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    266..290
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      333..585
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(605..1156)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(1199..1378)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     polyA_signal    1468..1642
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(2545..2565)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(2578..6681)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the N863A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKARGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(6688..6708)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(6805..7150)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(6842..6862)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        7354..7456
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             7457..8116
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(7523..7542)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             8462..8767
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     8646..8665
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     misc_RNA        8805..8880
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    9194..9218
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             10519..11148
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             11582..12649
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      12715..12909
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(13145..13167)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    13253..13393
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     13308..13327
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(13408..13425)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13579..14167)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13659..13678)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(14411..15202)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ttatgtccac tgggttcgtg cccgaattga tcacaggcag caacgctctg tcatcgttac
       61 aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa acccggcagc ttagttgccg
      121 ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg gctctcccgc
      181 tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc
      241 ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac aaattgacgc
      301 ttagacaact taataacaca ttgcggaatt ccgatctagt aacatagatg acaccgcgcg
      361 cgataattta tcctagtttg cgcgctatat tttgttttct atcgcgtatt aaatgtataa
      421 ttgcgggact ctaatcataa aaacccatct cataaataac gtcatgcatt acatgttaat
      481 tattacatgc ttaacgtaat tcaacagaaa ttatatgata atcatcgcaa gaccggcaac
      541 aggattcaat cttaagaaac tttattgcca aatgtttgaa cgatctgctt gactctaggg
      601 gtcatcagat ttcggtgacg ggcaggaccg gacggggcgg caccggcagg ctgaagtcca
      661 gctgccagaa acccacgtca tgccagttcc cgtgcttgaa gccggccgcc cgcagcatgc
      721 cgcggggggc atatccgagc gcctcgtgca tgcgcacgct cgggtcgttg ggcagcccga
      781 tgacagcgac cacgctcttg aagccctgtg cctccaggga cttcagcagg tgggtgtaga
      841 gcgtggagcc cagtcccgtc cgctggtggc ggggggagac gtacacggtc gactcggccg
      901 tccagtcgta ggcgttgcgt gccttccagg ggcccgcgta ggcgatgccg gcgacctcgc
      961 cgtccacctc ggcgacgagc cagggatagc gctcccgcag acggacgagg tcgtccgtcc
     1021 actcctgcgg ttcctgcggc tcggtacgga agttgaccgt gcttgtctcg atgtagtggt
     1081 tgacgatggt gcagaccgcc ggcatgtccg cctcggtggc acggcggatg tcggccgggc
     1141 gtcgttctgg gctcatggta gatccccctc gatcgagttg agagtgaata tgagactcta
     1201 attggatacc gaggggaatt tatggaacgt cagtggagca tttttgacaa gaaatatttg
     1261 ctagctgata gtgaccttag gcgacttttg aacgcgcaat aatggtttct gacgtatgtg
     1321 cttagctcat taaactccag aaacccgcgg ctgagtggct ccttcaacgt tgcggttctg
     1381 tcagttccaa acgtaaaacg gcttgtcccg cgtcatcggc gggggtcata acgtgactcc
     1441 cttaattctc cgctcatgat tagtagcgat ctggatttta gtactggatt ttggttttag
     1501 gaattagaaa ttttattgat agaagtattt tacaaataca aatacatact aagggtttct
     1561 tatatgctca acacatgagc gaaaccctat aggaacccta attcccttat ctgggaacta
     1621 ctcacacatt attatggaga aactcgagct tgtcgatcga ctctagctag agaagcttca
     1681 ctgaggcaat agctccatca accatgcgag ctcctcttcg ttgaatgccg ggaaatcatc
     1741 cgctgtccct gcgaaggggt ccgcatcttg ttcccaaacc acggtgctgg acgctaccag
     1801 gtcggcatcc atgggcgtac caggatccag gaggccgccg atcctggtac gcgggcgttt
     1861 tacaccccag ctgaggaggg gcaaatgcaa cgcatcgcgc tgttcgggaa cgcgcacctc
     1921 gaacgattgc tgtgcggagg gaccggtgac agcacgatcc gatcgggacc gtttacggct
     1981 gcttgcccgc gtctgatctc cctcgtgcat tgggctaggc gcatcaaggt cacgctccag
     2041 cgaatcggcg aatgcatgca aagacgcctg atccggcgtt tgagttgaag taggggacgg
     2101 tttggccctt ttcatccctg atgcctggag gatacggtcc caacgctgcg aggctggggg
     2161 gagcgttcca ctgcgggctt cgagttcggt gacgcccact ctgcgaaaga gctgtaacaa
     2221 cccgtgcctg ctcatcccga actgcgtcat ggcgtcatca aatgcttgcg ctgggtggga
     2281 gtggcactgg aaaaaaccca gcacgcgaac cacttgcgcg tggtcggcaa cgcgatggga
     2341 tgtgcgttcg ggaatacggc gattggttct tttgatcaag gccggcgcgt gcggcaatcc
     2401 ctttttcact gcatccagcg caggacgtcc gccgaggcag gccaaggcga cgaggtggtc
     2461 gttggtcaac gcggccaacg ccggatcagg gcgagataac tgggcaacaa tgcttccacc
     2521 gccaccacta ccgccacccg aacccacctt cctcttcttc ttggggtcag ccctgctgtc
     2581 tccaccgagc tgagagaggt cgattcttgt ttcatagagc cccgtaattg actgatgaat
     2641 cagtgtggcg tccaggacct cctttgtaga ggtgtaccgc tttctgtcta tggtggtgtc
     2701 gaagtacttg aaggctgcag gcgcgcccaa gttggtcaga gtaaacaagt ggataatgtt
     2761 ttctgcctgc tccctgatgg gcttatccct gtgcttattg taagcagaaa gcaccttatc
     2821 gaggttagcg tcggcgagga tcactctttt ggagaattcg cttatttgct cgatgatctc
     2881 atcaaggtag tgtttgtgtt gttccacgaa cagctgcttc tgctcattat cttcgggaga
     2941 tcctttgagc ttttcatagt ggctggccag atacaagaaa ttaacgtatt tagagggcag
     3001 tgccagctcg ttacctttct gcagctcgcc cgcactagcg agcattcgtt tccggccgtt
     3061 ttcaagctca aagagagagt acttgggaag cttaatgatg aggtcttttt tgacctcttt
     3121 atatcctttc gcctcgagaa agtcgatggg gtttttttcg aagcttgatc gctccatgat
     3181 tgtgatgccc agcagttcct tgacgctttt gagtttttta gacttccctt tctccacttt
     3241 ggccacaacc agtacactgt aagcgactgt aggagaatcg aatccgccgt atttcttggg
     3301 gtcccaatct tttttgcgtg cgatcagctt gtcgctgttc cttttcggga ggatactttc
     3361 cttggagaag cctccggtct gtacttcggt ctttttaacg atgttcacct gcggcatgga
     3421 caggaccttc cggactgtcg cgaaatccct acccttgtcc cacacgattt ctcctgtttc
     3481 tccgtttgtt tcgataagtg gtcgcttccg aatctctcca ttggccagtg taatctcggt
     3541 cttgaaaaaa ttcataatat tgctgtaaaa gaagtactta gcggtggcct tgcctatttc
     3601 ctgctcagac tttgcgatca ttttcctaac atcgtacact ttatagtctc cgtaaacaaa
     3661 ttcagattca agcttgggat attttttgat aagtgcagtg cctaccactg cattcaggta
     3721 ggcatcatgc gcatggtggt aattgttgat ctctctcacc ttataaaact gaaagtcctt
     3781 tctgaaatct gaaaccagct tagacttcag agtaataact ttcacctctc gaatcagttt
     3841 gtcattttca tcgtacttgg tgttcatgcg tgaatcgaga atttgggcca cgtgcttggt
     3901 gatctggcgt gtctcaacaa gctgcctttt gatgaagccg gctttatcca actcagacag
     3961 gccacctcgt tcagccttag tcagattatc gaacttccgt tgtgtgatca gtttggcgtt
     4021 cagcagctgc cgccaataat ttttcatttt cttgacaact tcttctgagg ggacgttatc
     4081 actcttccct ctagctttat cggatcttgt caacacttta ttatcaatag aatcatcttt
     4141 gagaaaagac tggggcacga tatgatccac gtcgtagtcg gagagccgat tgatgtccag
     4201 ttcctgatcc acgtacatgt ccctgccgtt ctgcaggtag tacaggtaga gcttctcatt
     4261 ctgaagctgg gtgttttcaa ctgggtgttc cttaaggatt tgggacccca gttcttttat
     4321 accctcttca atcctcttca tcctttccct actgttcttc tgtcccttct gggtagtttg
     4381 gttctctcgg gccatctcga taacgatatt ctcgggctta tgccttccca ttactttgac
     4441 gagttcatcc acgaccttaa cggtctgcag tattcccttt ttgatagctg ggctacctgc
     4501 aagattagcg atgtgctcgt ggagactgtc cccctggcca gaaacttgtg ctttctggat
     4561 gtcctcctta aaggtgagag agtcatcatg gatcaactgc atgaagttcc ggttggcaaa
     4621 tccatcggac ttaagaaaat ccaggattgt ctttccactc tgcttgtctc ggatcccatt
     4681 gatcagtttt cttgacagcc gcccccatcc tgtatatcgg cgcctcttga gctgtttcat
     4741 gactttgtcg tcgaagagat gagcgtaagt tttcaagcgt tcttcaatca tctccctatc
     4801 ttcaaacaac gtaagggtga ggacaatgtc ctcaagaatg tcctcgttct cctcattgtc
     4861 caggaagtcc ttgtctttaa tgattttcag gagatcgtga tacgttccca gggatgcgtt
     4921 gaagcgatcc tccactccgc tgatttcaac agagtcgaaa cattcaatct ttttgaaata
     4981 atcttctttg agctgtttca cggtaacttt ccggttcgtc ttgaagagga ggtccacgat
     5041 agctttcttc tgctctccag acaggaatgc tggctttctc atcccttctg tgacgtattt
     5101 gaccttggtg agctcgttat aaactgtgaa gtactcgtac agcagagagt gtttaggaag
     5161 caccttttcg ttaggcagat ttttatcaaa gttagtcatc ctttcgatga aggactgggc
     5221 agaggccccc ttatccacga cttcctcgaa gttccaggga gtgatagtct cttctgattt
     5281 gcgagtcatc cacgcgaatc tggaatttcc ccgggcgagg gggcctacat agtagggtat
     5341 ccgaaatgtg aggattttct caatcttttc cctgttatct ttcaaaaagg ggtagaaatc
     5401 ctcttgccgc ctgaggatag cgtgcagttc gcccaggtga atctggtggg ggatgcttcc
     5461 attgtcgaaa gtgcgctgtt tgcgcaacag atcttctctg ttaagcttta ccagcagctc
     5521 ctcggtgccg tccatttttt ccaagatggg cttaataaat ttgtaaaatt cctcctggct
     5581 tgctccgccg tcaatgtatc cggcgtagcc atttttagac tgatcgaaga aaatttcctt
     5641 gtacttctca ggcagttgct gtctgacaag ggccttcagc aaagtcaagt cttggtggtg
     5701 ctcatcatag cgcttgatca tactagcgct cagcggagct ttggtgatct ccgtgttcac
     5761 tcgcagaata tcactcagca gaatggcgtc tgacaggttc tttgccgcca aaaaaaggtc
     5821 tgcgtactgg tcgccgatct gggccagcag attgtcgaga tcatcatcgt aggtgtcttt
     5881 gctcagttga agcttggcat cttcggccag gtcgaagtta gatttaaagt tgggggtcag
     5941 cccgagtgac agggcgataa gattaccaaa caggccgttc ttcttctccc cagggagctg
     6001 tgcgatgagg ttttcgagcc gccgggattt ggacagccta gcgctcagga ttgctttggc
     6061 gtcaactccg gatgcgttga tcgggttctc ttcgaaaagc tgattgtaag tctgaaccag
     6121 ttggataaag agtttgtcga catcgctgtt gtctgggttc aggtccccct cgatgaggaa
     6181 gtgtccccga aatttgatca tatgcgccag cgcgagatag atcaaccgca agtcagcctt
     6241 atcagtactg tctacaagct tcttcctcag atgatatatg gttgggtact tttcatggta
     6301 cgccacctcg tccacgatat tgccaaagat tgggtggcgc tcgtgctttt tatcctcctc
     6361 caccaaaaag gactcctcca gcctatggaa gaaagagtca tccaccttag ccatctcatt
     6421 actaaagatc tcctgcaggt agcagatccg attctttctg cgggtatatc tgcgccgtgc
     6481 tgttcttttg agccgcgtgg cttcggccgt ctccccggag tcgaacagga gggcgccaat
     6541 gaggttcttc tttatgctgt ggcgatcggt attgcccaga actttgaatt ttttgctcgg
     6601 caccttgtac tcgtccgtaa tgacggccca gccgacgctg tttgtgccga tagcgagccc
     6661 aatggagtac ttcttgtcca tgccaccaac cttccgcttc ttcttaggag ccattgtatc
     6721 gataattgta aatgtaattg taatgttgtt tgttgtttgt tgttgttggt aattgttgta
     6781 aaaatgagct cttatactcg agcgtgtcct ctccaaatga aatgaacttc cttatataga
     6841 ggaagggtct tgcgaaggat agtgggattg tgcgtcatcc cttacgtcag tggagatgtc
     6901 acatcaatcc acttgctttg tagacgtggt tggaacctct tctttttcca cgatgctcct
     6961 cgtgggtggg ggtccatctt tgggaccact gtcggcagag agatcttgaa tgatagcctt
     7021 tcctttatcg caatgatggc atttgtagga gccaccttcc ttttctactg tcctttcgat
     7081 gaagtgacag atagctgggc aatggaatcc gaggaggttt cccgaaatta tcctttgttg
     7141 aaaagtctca atagcccttt gatcttctga gactgtatct ttgacatttt tggagtagac
     7201 cagagtgtcg tgctccacca tgttgacctc caagcttgaa ttcttgctaa gttgaaaaca
     7261 atcttcaaaa gtcccacatc gcttagataa gaaaacgaag ctgagtttat atacagctag
     7321 agtcgaagta gtgattgaag tcttctgaga cgttgatcgg cacgtaagag gttccaactt
     7381 tcaccataat gaaataagat cactaccggg cgtatttttt gagttatcga gattttcagg
     7441 agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca
     7501 atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca
     7561 gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt
     7621 ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat
     7681 ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt
     7741 ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca
     7801 gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc
     7861 taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag
     7921 ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa
     7981 atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt
     8041 ctgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg
     8101 gcaggggggg gcgtaaacgc tcgcgtggat ccggcttact aaaagccaga taacagtatg
     8161 cgtatttgcg cgctgatttt tgcggtataa gaatatatac tgatatgtat acccgaagta
     8221 tgtcaaaaag aggtatgcta tgaagcagcg tattacagtg acagttgaca gcgacagcta
     8281 tcagttgctc aaggcatata tgatgtcaat atctccggtc tggtaagcac aaccatgcag
     8341 aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg aaaatcagga agggatggct
     8401 gaggtcgccc ggtttattga aatgaacggc tcttttgctg acgagaacag gggctggtga
     8461 aatgcagttt aaggtttaca cctataaaag agagagccgt tatcgtctgt ttgtggatgt
     8521 acagagtgat attattgaca cgcccgggcg acggatggtg atccccctgg ccagtgcacg
     8581 tctgctgtca gataaagtct cccgtgaact ttacccggtg gtgcatatcg gggatgaaag
     8641 ctggcgcatg atgaccaccg atatggccag tgtgccggtt tccgttatcg gggaagaagt
     8701 ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg
     8761 aatataaatg tcaggctccc ttatacacag ccagtcgaag acttgtttta gagctagaaa
     8821 tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc
     8881 ttttttttgc ttccatgcta gagtccgcaa aaatcaccag tctctctcta caaatctatc
     8941 tctctctatt tttctccaga ataatgtgtg agtagttccc agataaggga attagggttc
     9001 ttatagggtt tcgctcatgt gttgagcata taagaaaccc ttagtatgta tttgtatttg
     9061 taaaatactt ctatcaataa aatttctaat tcctaaaacc aaaatccagt gacctgcagg
     9121 catgcgacgt cgagcttagc ttgagcttgg atcagattgt cgtttcccgc cttcagttta
     9181 aactatcagt gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt
     9241 agaataatcg gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg
     9301 catgccaacc acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct
     9361 atagtgcagt cggcttctga cgttcagtgc agccgtgttc tgaaaacgac atgtcgcaca
     9421 agtcctaagt tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt
     9481 gttttagtcg cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca
     9541 agagcgccgc cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga
     9601 ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca
     9661 ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg
     9721 acgttgtgac agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca
     9781 ttgccgagcg catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg
     9841 acaccaccac gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg
     9901 agcgttccct aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg
     9961 tgaagtttgg cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga
    10021 tcgaccagga aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga
    10081 ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg
    10141 gtgccttccg tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac
    10201 gccaagagga acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac
    10261 cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt
    10321 ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg
    10381 gccggccagc ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt
    10441 tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca
    10501 aatacgcaag gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc
    10561 aagacgacca tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg
    10621 ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa
    10681 ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc
    10741 cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg
    10801 atcaaggcag ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc
    10861 accgccgacc tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa
    10921 gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag
    10981 gcgctggccg ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac
    11041 ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc
    11101 cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta
    11161 aagagaaaat gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca
    11221 gcaaggctgc aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc
    11281 agttgccggc ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca
    11341 ttaccgagct gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa
    11401 atgagtagat gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc
    11461 accgacgccg tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc
    11521 tgggttgtct gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga
    11581 cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga
    11641 gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg
    11701 tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc
    11761 cggtgcgccg tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc
    11821 gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg
    11881 tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca
    11941 cgtagaggtt tccgcagggc cggccggcat ggccagtgtg tgggattacg acctggtact
    12001 gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa
    12061 gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga
    12121 tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt
    12181 tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga
    12241 agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga
    12301 gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct
    12361 gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct
    12421 ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg
    12481 cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc
    12541 aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt
    12601 catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat gtacggagca
    12661 gatgctaggg caaattgccc tagcagggga aaaaggtcga aaaggtgtct ttcctgtgga
    12721 tagcacgtac attgggaacc caaagccgta cattgggaac cggaacccgt acattgggaa
    12781 cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa aagagaaaaa
    12841 aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa cccgcctggc
    12901 ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc ctacccttcg
    12961 gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc
    13021 aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg cgccgtcgcc
    13081 actcgaccgc cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg
    13141 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
    13201 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
    13261 tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca
    13321 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
    13381 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
    13441 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
    13501 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
    13561 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
    13621 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
    13681 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
    13741 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
    13801 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
    13861 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
    13921 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
    13981 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
    14041 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
    14101 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
    14161 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
    14221 acgttaaggg attttggtca tgcatgatat atctcccaat ttgtgtaggg cttattatgc
    14281 acgcttaaaa ataataaaag cagacttgac ctgatagttt ggctgtgagc aattatgtgc
    14341 ttagtgcatc taatcgcttg agttaacgcc ggcgaagcgg cgtcggcttg aacgaatttc
    14401 tagctagaca ttatttgccg actaccttgg tgatctcgcc tttcacgtag tggacaaatt
    14461 cttccaactg atctgcgcgc gaggccaagc gatcttcttc ttgtccaaga taagcctgtc
    14521 tagcttcaag tatgacgggc tgatactggg ccggcaggcg ctccattgcc cagtcggcag
    14581 cgacatcctt cggcgcgatt ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa
    14641 gcactacatt tcgctcatcg ccagcccagt cgggcggcga gttccatagc gttaaggttt
    14701 catttagcgc ctcaaataga tcctgttcag gaaccggatc aaagagttcc tccgccgctg
    14761 gacctaccaa ggcaacgcta tgttctcttg cttttgtcag caagatagcc agatcaatgt
    14821 cgatcgtggc tggctcgaag atacctgcaa gaatgtcatt gcgctgccat tctccaaatt
    14881 gcagttcgcg cttagctgga taacgccacg gaatgatgtc gtcgtgcaca acaatggtga
    14941 cttctacagc gcggagaatc tcgctctctc caggggaagc cgaagtttcc aaaaggtcgt
    15001 tgatcaaagc tcgccgcgtt gtttcatcaa gccttacggt caccgtaacc agcaaatcaa
    15061 tatcactgtg tggcttcagg ccgccatcca ctgcggagcc gtacaaatgt acggccagca
    15121 acgtcggttc gagatggcgc tcgatgacgc caactacctc tgatagttga gtcgatactt
    15181 cggcgatcac cgcttccccc atgatgttta actttgtttt agggcgactg ccctgctgcg
    15241 taacatcgtt gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct
    15301 tggatgcccg aggcatagac tgtaccccaa aaaaacatgt cataacaaga agccatgaaa
    15361 accgccactg cgccgttacc accgctgcgt tcggtcaagg ttctggacca gttgcgtgac
    15421 ggcagttacg ctacttgcat tacagcttac gaaccgaacg aggc
//
