LOCUS Exported 7732 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION cpstFRET actin sensor for mechanical force measurements in cytoskeleton. ACCESSION . VERSION . KEYWORDS Actin-cpstFRET-Actin SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7732) AUTHORS Guo J, Wang Y, Sachs F, Meng F TITLE Actin stress in cell reprogramming. JOURNAL Proc Natl Acad Sci U S A. 2014 Dec 9;111(49):E5252-61. doi: 10.1073/pnas.1411683111. Epub 2014 Nov 24. PUBMED 25422450 REFERENCE 2 (bases 1 to 7732) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7732 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 61..364 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 365..568 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 519..539 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" CDS 1965..2480 /codon_start=1 /product="N-terminal fragment of mVenus for use in bimolecular fluorescence complementation (BiFC) (Kodama and Hu, 2010)" /label=VN173 /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA NFKIRHNIE" polyA_signal 4520..4641 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(4557..4576) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 4611..4630 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(4648..5103) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4785..4806) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 4997..5016 /label=F1ori-R /note="F1 origin, reverse primer" promoter 5130..5234 /gene="bla" /label=AmpR promoter promoter 5236..5593 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(5259..5279) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 5444..5579 /label=SV40 ori /note="SV40 origin of replication" primer_bind 5506..5525 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 5628..6422 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(5682..5701) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 6292..6311 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(6610..6629) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 6654..6701 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 7030..7618 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 7519..7538 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcatgg 601 atgatgatat cgccgcgctc gtcgtcgaca acggctccgg catgtgcaag gccggcttcg 661 cgggcgacga tgccccccgg gccgtcttcc cctccatcgt ggggcgcccc aggcaccagg 721 gcgtgatggt gggcatgggt cagaaggatt cctatgtggg cgacgaggcc cagagcaaga 781 gaggcatcct caccctgaag taccccatcg agcacggcat cgtcaccaac tgggacgaca 841 tggagaaaat ctggcaccac accttctaca atgagctgcg tgtggctccc gaggagcacc 901 ccgtgctgct gaccgaggcc cccctgaacc ccaaggccaa ccgcgagaag atgacccaga 961 tcatgtttga gaccttcaac accccagcca tgtacgttgc tatccaggct gtgctatccc 1021 tgtacgcctc tggccgtacc actggcatcg tgatggactc cggtgacggg gtcacccaca 1081 ctgtgcccat ctacgagggg tatgccctcc cccatgccat cctgcgtctg gacctggctg 1141 gccgggacct gactgactac ctcatgaaga tcctcaccga gcgcggctac agcttcacca 1201 ccacggccga gcgggaaatc gtgcgtgaca ttaaggagaa gctgtgctac gtcgccctgg 1261 acttcgagca agagatggcc acggctgctt ccagctcctc cctggagaag agctacgagc 1321 tgcctgacgg ccaggtcatc accattggca atgagcggtt ccgctgccct gaggcactct 1381 tccagccttc cttcctgggc atggagtcct gtggcatcca cgaaactacc ttcaactcca 1441 tcatgaagtg tgacgtggac atccgcaaag acctgtacgc caacacagtg ctgtctggcg 1501 gcaccaccat gtaccctggc attgccgaca ggatgcagaa ggagatcact gccctggcac 1561 ccagcacaat gaagatcaag atcattgctc ctcctgagcg caagtactcc gtgtggatcg 1621 gcggctccat cctggcctcg ctgtccacct tccagcagat gtggatcagc aagcaggagt 1681 atgacgagtc cggcccctcc atcgtccacc gcaaatgctt cggaggaggc ggggtaccag 1741 gatccatggg cggcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg 1801 gccccgtgct gctgcccgac aaccactacc tgagctacca gtccaaactg agcaaagacc 1861 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc 1921 tcggcatgga cgagctgtac aaggaattcg gcggcggtgg cggggtgagc aagggcgagg 1981 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca 2041 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagc 2101 tgatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgggct 2161 acggcctgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt 2221 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact 2281 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga 2341 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca 2401 acagccacaa cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca 2461 agatccgcca caacatcgag gacggcggca gatctatggg cagcgtgcag ctcgccgacc 2521 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc 2581 tgagcaccca gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc 2641 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aaggaattcg 2701 gcggcggtgg cggggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg 2761 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg 2821 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc 2881 cctggcccac cctcgtgacc accctgacct ggggcgtgca gtgcttcgcc cgctaccccg 2941 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc 3001 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg 3061 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca 3121 tcctggggca caagctggag tacaacgcca tcagcgacaa cgtctatatc accgccgaca 3181 agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag gacgagctcg 3241 gtgggggagg aggtatggat gatgatatcg ccgcgctcgt cgtcgacaac ggctccggca 3301 tgtgcaaggc cggcttcgcg ggcgacgatg ccccccgggc cgtcttcccc tccatcgtgg 3361 ggcgccccag gcaccagggc gtgatggtgg gcatgggtca gaaggattcc tatgtgggcg 3421 acgaggccca gagcaagaga ggcatcctca ccctgaagta ccccatcgag cacggcatcg 3481 tcaccaactg ggacgacatg gagaaaatct ggcaccacac cttctacaat gagctgcgtg 3541 tggctcccga ggagcacccc gtgctgctga ccgaggcccc cctgaacccc aaggccaacc 3601 gcgagaagat gacccagatc atgtttgaga ccttcaacac cccagccatg tacgttgcta 3661 tccaggctgt gctatccctg tacgcctctg gccgtaccac tggcatcgtg atggactccg 3721 gtgacggggt cacccacact gtgcccatct acgaggggta tgccctcccc catgccatcc 3781 tgcgtctgga cctggctggc cgggacctga ctgactacct catgaagatc ctcaccgagc 3841 gcggctacag cttcaccacc acggccgagc gggaaatcgt gcgtgacatt aaggagaagc 3901 tgtgctacgt cgccctggac ttcgagcaag agatggccac ggctgcttcc agctcctccc 3961 tggagaagag ctacgagctg cctgacggcc aggtcatcac cattggcaat gagcggttcc 4021 gctgccctga ggcactcttc cagccttcct tcctgggcat ggagtcctgt ggcatccacg 4081 aaactacctt caactccatc atgaagtgtg acgtggacat ccgcaaagac ctgtacgcca 4141 acacagtgct gtctggcggc accaccatgt accctggcat tgccgacagg atgcagaagg 4201 agatcactgc cctggcaccc agcacaatga agatcaagat cattgctcct cctgagcgca 4261 agtactccgt gtggatcggc ggctccatcc tggcctcgct gtccaccttc cagcagatgt 4321 ggatcagcaa gcaggagtat gacgagtccg gcccctccat cgtccaccgc aaatgcttct 4381 aaagggcccg ggatccaccg gatctagata actgatcata atcagccata ccacatttgt 4441 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat 4501 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa 4561 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc 4621 caaactcatc aatgtatctt aaggcgtaaa ttgtaagcgt taatattttg ttaaaattcg 4681 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc 4741 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga 4801 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg 4861 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag 4921 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga 4981 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg 5041 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg 5101 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa 5161 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt 5221 gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg 5281 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc 5341 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca 5401 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc 5461 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc 5521 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct 5581 aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag 5641 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg 5701 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc 5761 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag 5821 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca 5881 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat 5941 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata 6001 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac 6061 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc 6121 tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg 6181 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg 6241 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta 6301 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg 6361 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct 6421 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga 6481 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc 6541 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg 6601 gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat 6661 aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc 6721 ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt 6781 ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa 6841 cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt 6901 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac 6961 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa 7021 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc 7081 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt 7141 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg 7201 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc 7261 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt 7321 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga 7381 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct 7441 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg 7501 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca 7561 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa 7621 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt 7681 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgccatgc at //