LOCUS       Exported                 180 bp ds-DNA     linear   SYN 13-JUN-2021
DEFINITION  pBIG2ab - Step2 vector ab (biGBac system for expression of protein 
            complexes in insect cells).
ACCESSION   .
VERSION     .
KEYWORDS    pBIG2ab
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 180)
  AUTHORS   Weissmann F, Petzold G, VanderLinden R, Huis In 't Veld PJ, Brown 
            NG, Lampert F, Westermann S, Stark H, Schulman BA, Peters JM
  TITLE     biGBac enables rapid gene assembly for the expression of large 
            multisubunit protein complexes.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 May 10;113(19):E2564-9. doi: 
            10.1073/pnas.1604935113. Epub 2016 Apr 25.
  PUBMED    27114506
REFERENCE   2  (bases 1 to 180)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..180
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    1..34
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
ORIGIN
        1 ataacttcgt atagcataca ttatacgaag ttatctggcc taggttaatt aaacgctcta
       61 tggtctaaag tttaaactgg atactattgc acgtttcaat caggagatcc gaaccagata
      121 agtgaaatct agttccaaac tattttgtca tttttaattt tcgtattagc ttacgacgct
//
