LOCUS Exported 6448 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Localization: Focal Adhesions, Excitation: 587, Emission: 610. ACCESSION . VERSION . KEYWORDS mCherry-Zyxin-C-13 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6448) TITLE Davidson lab - mCherry constructs REFERENCE 2 (bases 1 to 6448) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6448 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 68..371 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 372..575 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 526..546 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 614..623 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 620..1327 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /label=mCherry /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" primer_bind complement(765..783) /label=mCherry-R /note="mCherry, reverse primer" primer_bind 1033..1052 /label=mCherry-F /note="mCherry, forward primer" regulatory 1362..1371 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" polyA_signal 3243..3364 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3280..3299) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 3334..3353 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(3371..3826) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3508..3529) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 3720..3739 /label=F1ori-R /note="F1 origin, reverse primer" promoter 3853..3957 /gene="bla" /label=AmpR promoter promoter 3959..4316 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(3982..4002) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 4167..4302 /label=SV40 ori /note="SV40 origin of replication" primer_bind 4229..4248 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 4351..5145 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(4405..4424) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 5015..5034 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(5333..5352) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 5377..5424 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 5753..6341 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6242..6261 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg 61 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc 121 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt 181 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc 241 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 301 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 361 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 421 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 481 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 541 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct 601 agcgctaccg gtcgccacca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa 661 ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat 721 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac 781 caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc 841 caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga 901 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca 961 ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt 1021 cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg 1081 gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga 1141 cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct 1201 gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac 1261 catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct 1321 gtacaagtcc ggactcagat ctcgagctca agcttcgaat tccatggcgg ccccccgccc 1381 gtctcccgcg atccccgttt cggtctcggc tccggctttt tacgccccgc agaagaagtt 1441 cggccctgtg gtggccccaa agcccaaagt gaatcccttc cggcccaggg acagcgagcc 1501 tcccccggca cccggggccc agcgcgcaca gatgggccgg gtgggcgata ttcccccgcc 1561 gcccccggaa gactttcccc tgcctccacc tccccttgct ggggatggcg acgatgcaga 1621 gggtgctctg ggaggtgcct tcccgccgcc ccctcccccg atcgaggaat catttccccc 1681 tgcgcctctg gaggaggaga tcttcccttc cccgccgcct cctccggagg aggagggagg 1741 gcctgaggcc cccataccgc ccccaccaca gcccagggag aaggtgagca gtattgattt 1801 ggagatcgac tctctgtcct cactgctgga tgacatgacc aagaatgatc ctttcaaagc 1861 ccgggtgtca tctggatatg tgcccccacc agtggccact ccattcagtt ccaagtccag 1921 taccaagcct gcagccgggg gcacagcacc cctgcctcct tggaagtccc cttccagctc 1981 ccagcctctg ccccaggttc cggctccggc tcagagccag acacagttcc atgttcagcc 2041 ccagccccag cccaagcctc aggtccaact ccatgtccag tcccagaccc agcctgtgtc 2101 tttggctaac acccagcccc gagggccccc agcctcatct ccggctccag cccctaagtt 2161 ttctccagtg actcctaagt ttactcctgt ggcttccaag ttcagtcctg gagccccagg 2221 tggatctggg tcacaaccaa atcaaaaatt ggggcacccc gaagctcttt ctgctggcac 2281 aggctcccct caacctccca gcttcaccta tgcccagcag agggagaagc cccgagtgca 2341 ggagaagcag caccccgtgc ccccaccggc tcagaaccaa aaccaggtgc gctcccctgg 2401 ggccccaggg cccctgactc tgaaggaggt ggaggagctg gagcagctga cccagcagct 2461 aatgcaggac atggagcatc ctcagaggca gaatgtggct gtcaacgaac tctgcggccg 2521 atgccatcaa cccctggccc gggcgcagcc agccgtccgc gctctagggc agctgttcca 2581 catcgcctgc ttcacctgcc accagtgtgc gcagcagctc cagggccagc agttctacag 2641 tctggagggg gcgccgtact gcgagggctg ttacactgac accctggaga agtgtaacac 2701 ctgcggggag cccatcactg accgcatgct gagggccacg ggcaaggcct atcacccgca 2761 ctgcttcacc tgtgtggtct gcgcccgccc cctggagggc acctccttca tcgtggacca 2821 ggccaaccgg ccccactgtg tccccgacta ccacaagcag tacgccccga ggtgctccgt 2881 ctgctctgag cccatcatgc ctgagcctgg ccgagatgag actgtgcgag tggtcgccct 2941 ggacaagaac ttccacatga agtgttacaa gtgtgaggac tgcgggaagc ccctgtcgat 3001 tgaggcagat gacaatggct gcttccccct ggacggtcac gtgctctgtc ggaagtgcca 3061 cactgctaga gcccagacct aagaattctg cagtcgacgg taccgcgggc ccgggatcca 3121 ccggatctag ataactgatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt 3181 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg 3241 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca 3301 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat 3361 cttaacgcgt aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa 3421 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa 3481 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac 3541 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa 3601 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct 3661 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa 3721 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc 3781 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt 3841 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat 3901 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtcct 3961 gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct 4021 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa 4081 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa 4141 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt 4201 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 4261 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaagatc 4321 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt 4381 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc 4441 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag 4501 accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg 4561 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac 4621 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc 4681 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc 4741 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc 4801 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg 4861 ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat 4921 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc 4981 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa 5041 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat 5101 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt 5161 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg 5221 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc 5281 agcgcgggga tctcatgctg gagttcttcg cccaccctag ggggaggcta actgaaacac 5341 ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa 5401 cgcacggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg 5461 tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac 5521 cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc 5581 ctgccatagc ctcaggttac tcatatatac tttagattga tttaaaactt catttttaat 5641 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg 5701 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc 5761 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg 5821 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag 5881 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact 5941 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg 6001 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc 6061 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg 6121 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg 6181 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag 6241 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc 6301 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct 6361 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc 6421 ctgattctgt ggataaccgt attaccgc //