LOCUS Exported 12344 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS myc mTOR kinase dead SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12344) TITLE myc mTOR kinase dead REFERENCE 2 (bases 1 to 12344) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12344 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 107..128 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 143..173 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 181..197 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 186..208 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 205..221 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 205..221 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" enhancer 250..629 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 630..833 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 784..804 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 830..854 /label=LNCX /note="Human CMV promoter, forward primer" promoter 1064..1082 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind 1064..1081 /label=SP6 /note="SP6 promoter, forward primer" primer_bind 1095..1114 /label=pMT2-F /note="Synthetic intron, forward primer" regulatory 1160..1169 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 1169..1198 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" regulatory 4053..4062 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 7274..7546 /codon_start=1 /product="FKBP-rapamycin binding domain of human FRAP" /label=FRB /translation="EMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETS FNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQL" regulatory 8880..8889 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" polyA_signal 8893..9027 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(8930..8949) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 8984..9003 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" promoter 9096..9453 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(9119..9139) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 9304..9439 /label=SV40 ori /note="SV40 origin of replication" primer_bind 9366..9385 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(9473..9490) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(9473..9489) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(9482..9504) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 9702..10157 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(9789..9808) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 9999..10020 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(10174..10193) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 10293..10315 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(10353..10371) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 10439..10543 /gene="bla" /label=AmpR promoter CDS 10544..11404 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(10762..10781) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 11575..12163 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 12064..12083 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 12317..12334 /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 181 ttgtgagcgg ataacaattt cacacaggaa acagctatga catgattacg aattaattcg 241 agctcgcccg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag 301 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct 361 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc 421 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg 481 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat 541 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 601 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 661 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 721 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 781 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag 841 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc 901 gggaccgatc cagcctccgc ggccgggaac ggtgcattgg aacgcggatt ccccgtgcca 961 agagtgacgt aagtaccgcc tatagagtct ataggcccac ccccttggct tcgttagaac 1021 gcggctacaa ttaatacata accttatgta tcatacacat acgatttagg tgacactata 1081 gaataacatc cactttgcct ttctctccac aggtgtccac tcccaggtcc aactgcacct 1141 cggttctatc gataagcttg ccaccatgga acaaaagctc attagcgagg aagaccttaa 1201 catgcttggg acaggccctg ccacggccac cgccggtgcc gccacatcta gcaacgtgag 1261 cgttctgcag cagttcgcca gtgggctgaa gagccggaat gaggagacca gggccaaagc 1321 agccaaggag ctccagcact atgtcaccat ggaacttcga gagatgagtc aggaggagtc 1381 tactcgcttc tatgaccagc tgaaccatca catttttgaa ctggtttcca gctcagacgc 1441 caatgagagg aagggtggca tcttggccat tgccagcctc attggagtgg aaggtgggaa 1501 ttccaccagg attggcagat ttgccaacta ccttcggaac ctcctcccct caagtgatcc 1561 agttgtcatg gagatggcat ccaaggccat tggccgcctt gcaatggcag gggacacttt 1621 cactgctgag tatgtggagt ttgaagtgaa gcgagccttg gagtggctgg gtgctgaccg 1681 aaatgagggc cggagacatg cagctgtcct cgttctccgt gagctggcca tcagcgtccc 1741 caccttcttc ttccagcaag ttcagccctt ctttgacaac atttttgtgg ctgtgtggga 1801 ccccaagcag gccatccgtg aaggagctgt ggctgccctt cgtgcctgtc tgattcttac 1861 cacgcagcgg gagccgaagg agatgcagaa gcctcagtgg tacaggcaca catttgaaga 1921 agcagagaaa ggttttgatg agaccctggc caaagagaag ggtatgaacc gagatgatcg 1981 aatccacggg gccttgctga tcctcaacga gctcgtccga atcagcagca tggagggaga 2041 gcgtctgaga gaggagatgg aggaaatcac ccagcagcag ctggtacatg acaagtactg 2101 caaagaccta atgggctttg ggacaaagcc tcggcacatc actcccttca ccagcttcca 2161 ggctgtgcag ccccagcagt caaacgcctt ggtgggactg ctggggtaca gctcccacca 2221 aggcctaatg gggtttgggg cttcccccag ccctacaaag tccactctgg tggaaagccg 2281 ttgttgcaga gacttgatgg aagagaaatt tgatcaggtg tgccagtggg tgctgaaatg 2341 taggagcagc aagaactcac tgatccaaat gacaatcctt aatctgttgc cccgcttggc 2401 tgcattccga ccgtctgcct tcacagatac ccagtacctg caagacacca tgaaccatgt 2461 cctgagctgt gtcaagaagg aaaaggaacg gaccgcagcg ttccaggccc tagggctgct 2521 ttctgtggcg gtgaggtccg agtttaaggt ctacctgccc cgagtacttg acatcatccg 2581 agcagccctg cctccaaagg actttgccca caagaggcag aaaactgtgc aggtggatgc 2641 cacagtgttc acgtgcatca gcatgctggc gcgggccatg gggccaggca tccagcagga 2701 catcaaggag ctgctggagc ccatgttggc agtgggcctg agccctgcgc tcactgctgt 2761 gctctatgac ctgagccggc agattccgca gctgaagaaa gatattcagg acgggcttct 2821 gaagatgctg tccctggtcc ttatgcacaa acccctgcgg cacccgggca tgcccaaagg 2881 cctggcccac cagctggcct ccccaggtct taccaccctc cctgaggcca gcgacgtggc 2941 cagcatcact cttgcccttc gaactctcgg cagctttgaa tttgaaggcc actctctgac 3001 ccagttcgtc cgacactgcg cagatcattt cctgaacagt gagcacaagg agatccgcat 3061 ggaagcagcc cgcacctgct cccgcctgct cacaccctcc atccacctca tcagcggcca 3121 tgcccatgtg gttagccaga ccgcagtgca agtggtagca gatgtgctca gcaagctgct 3181 tgtggtcggc ataacagatc ctgatcctga tatccgctac tgtgtcttgg cctccctgga 3241 tgagcgcttc gatgcccact tggcccaggc agaaaactta caagctctgt ttgtggctct 3301 gaatgaccag gtctttgaga tccgagagct ggccatctgc actgtgggcc gactcagtag 3361 catgaaccca gcctttgtca tgcctttcct gcgcaagatg ctcatccaga ttttgacaga 3421 gctggagcac agtggcattg ggagaatcaa ggagcagagt gcccgcatgc tggggcacct 3481 ggtctccaat gccccccgcc tcatccgccc ctatatggag cctattctga aggctttaat 3541 tttgaaactc aaagatccag accctgaccc aaacccgggc gtgatcaata acgtgttggc 3601 cactatagga gaactggctc aggttagcgg cctggagatg aggaagtggg tggacgagct 3661 ctttgtcatc atcatggaca tgctgcagga ctcctctctt ctggccaaaa gacaggtggc 3721 tttgtggacc ctgggacagt tggtggccag tactggctac gtggtggagc cctacaggaa 3781 gtaccccact ctgcttgaag tgctgctgaa ttttctgaag acggagcaga accagggcac 3841 tcggagagag gccatccgag tgttagggct cctcggggct ttggacccct acaagcacaa 3901 agtgaacatc ggcatgattg accagtcccg agatgcttct gctgtcagcc tgtcagaatc 3961 caagtcaagt caagattcct ctgactacag caccagtgaa atgctggtca acatgggaaa 4021 cctgccactg gacgagttct accccgccgt gtccatggtg gccttgatgc ggatcttccg 4081 agaccagtcc ctctctcacc accacaccat ggtggttcag gccatcacct tcatcttcaa 4141 gtccctgggg ctcaagtgtg tgcagttcct gccccaggtc atgcccacgt tccttaacgt 4201 catccgagtc tgtgatgggg ccatccggga atttctgttc cagcagctgg gaatgctggt 4261 gtcctttgtg aagagccaca tccgtcccta catggatgaa atagtcaccc tcatgagaga 4321 attttgggtc atgaacacct caatccagag cacaatcatt cttctcattg agcaaatcgt 4381 ggtggctctt ggaggtgaat ttaagctcta cctgccccag ctgatcccac acatgctgcg 4441 tgtcttcatg catgacaaca gccagggccg catagtctcc atcaagctgt tagcagcgat 4501 ccagctgttt ggcgccaacc tggatgacta tctgcacttg ttgttgcctc cgatcgtgaa 4561 attgtttgat gcccctgaag ttccactgcc gtcgagaaag gcagcgttgg agacagtgga 4621 ccgcctgaca gagtccctgg atttcactga ctatgcctcc cgcatcattc acccgattgt 4681 tcgcacgcta gaccagagcc cagagctgcg ctccacagcc atggacaccc tgtcttcact 4741 tgtgtttcaa ctagggaaga agtaccagat cttcattcca atggtgaata aagtccttgt 4801 gcgacaccgg atcaatcacc agcgctacga cgtgctgatc tgcagaatcg tcaaggggta 4861 cacgcttgct gatgaagaag aagacccttt gatttaccag catcgaatgc taaggagcag 4921 ccagggagat gccctggcca gtggaccagt tgaaacagga cccatgaaga aactgcatgt 4981 cagcaccatc aacctccaaa aggcctgggg agctgccaga agggtctcca aggacgactg 5041 gctggagtgg ctgcgacgct tgagtctgga gctgctgaag gattcctcat caccttccct 5101 gcgctcatgc tgggccctgg cccaggccta caaccccatg gccagggatc tcttcaacgc 5161 tgcgtttgtg tcctgctggt ctgaactgaa tgaagaccaa caagatgagc tcatcaggag 5221 cattgagttg gctctcactt ctcaagacat tgctgaagtc acacaaaccc tcttgaactt 5281 ggctgagttc atggagcaca gtgacaaggg ccccctacca ctgagagatg acaatggcat 5341 cgtgctgttg ggtgagagag ctgccaagtg ccgggcatat gccaaagcac tacactacaa 5401 agagctggag ttccagaagg ggcccacgcc tgccatactt gagtccctca tcagcattaa 5461 taataaactg cagcagcctg aggcagcgtc cggggtgtta gagtacgcca tgaaacactt 5521 cggagagctg gagatccagg ccacctggta tgagaagttg catgagtggg aggacgccct 5581 tgtggcctac gacaagaaga tggacacgaa caaggatgac ccagagctga tgctgggccg 5641 catgcgctgt ctcgaggcct tgggagaatg gggccagctt catcagcagt gctgtgaaaa 5701 gtggactctg gttaatgacg agacccaggc taagatggcc cggatggctg ctgcagcagc 5761 atggggttta ggtcagtggg acagcatgga ggagtacacc tgtatgattc ctcgggatac 5821 tcacgatgga gcattctaca gagcagtgtt ggcactgcat caggatctct tctccttggc 5881 tcaacagtgc attgacaagg ccagggacct gctggacgcc gagctgactg ccatggcagg 5941 ggagagctac agccgagcct atggggccat ggtttcttgc cacatgctgt ccgagctgga 6001 ggaggttatc cagtacaaac tcgtcccgga gcgacgggag atcatccgcc agatctggtg 6061 ggagagactg cagggctgcc agcgtattgt agaggactgg cagaaaatcc tcatggtccg 6121 gtcccttgtg gtcagccctc acgaggacat gagaacctgg ctcaagtacg caagcctgtg 6181 tggcaagagc ggcagactgg ctcttgctca taaaacctta gtgttgctct tgggagttga 6241 tccatctcgg caacttgacc atcctctgcc aacagttcac cctcaagtga cctatgccta 6301 catgaaaaac atgtggaaaa gcgctcggaa gattgatgcc ttccagcaca tgcagcactt 6361 tgtgcagacc atgcagcagc aggcccagca cgccattgcc acagaggacc agcagcacaa 6421 gcaggagctg cataagctca tggccaggtg ttttctgaaa cttggggagt ggcagctgaa 6481 cctccagggc atcaacgaga gcaccatccc caaggtgcta cagtactaca gtgctgccac 6541 agagcatgac cgcagctggt ataaggcttg gcacgcatgg gcagtgatga actttgaagc 6601 cgtgctacac tacaaacatc agaaccaagc ccgcgatgag aagaagaaac tgcgccatgc 6661 cagcggggcc aacatcacca atgccaccac cactgccacc accgctgcct ccgctgccgc 6721 tgccaccagc acagagggca gcaacagtga aagtgaagcc gagagcaatg agagcagccc 6781 caccccgtcc cctctgcaga agaaggtcac tgaggatttg tccaaaaccc tcttgttgta 6841 cactgtccct gctgtccaag gcttcttccg ttctatctcc ttgtcgagag gcaacaacct 6901 ccaggataca ctcagagtcc tcaccttgtg gtttgattat ggtcactggc cagatgtcaa 6961 tgaagccctg gtggaagggg tgaaggccat acagattgac acttggttac aggttatacc 7021 tcagctcatt gcaagaattg acacgcccag acccttggtg ggccggctca ttcaccagct 7081 cctcacagat attggtcggt accacccaca ggccctcatc taccccctga cggtggcttc 7141 taagtctacc accacagccc gtcacaatgc agccaacaag atcctgaaga acatgtgcga 7201 gcacagcaac acgctagtcc agcaggccat gatggtgagt gaagagctga ttcgagtagc 7261 catcctctgg catgagatgt ggcatgaagg cctagaagag gcctctcgct tgtactttgg 7321 ggagaggaac gtcaaaggca tgtttgaggt gctggagccc ctgcatgcta tgatggaacg 7381 cggtccccag accctgaagg aaacgtcctt taatcaggca tatggtcgag atttaatgga 7441 ggcacaagaa tggtgccgaa agtacatgaa atcagggaac gtcaaggacc tcacccaagc 7501 ctgggacctc tactatcacg tgttcagacg gatctccaag cagctaccac agctcacatc 7561 cctggagctg cagtatgtgt cccccaaact tttgatgtgc agagaccttg aattggctgt 7621 gccaggaaca tatgacccca accagccaat cattcgcatt cagtccatag ccccgtcttt 7681 gcaagtcatc acatccaagc agaggcctcg gaagctgacc ctgatgggca gcaatgggca 7741 cgagtttgtt ttcctcctga aaggccatga agatctgcgg caggacgagc gagtgatgca 7801 gctctttggc ctggtgaaca cactcctagc caatgaccca acttctcttc gaaagaacct 7861 cagcatccag agatacgccg tcattcctct gtccaccaac tcgggcctga ttggctgggt 7921 gccccactgt gacacgctgc atgccctcat ccgggactac agagagaaga agaagatcct 7981 gctgaacatc gagcaccgca tcatgctgcg gatggctcct gactatgacc acctgactct 8041 gatgcagaag gtggaggtgt ttgagcatgc tgtcaacaac acagccgggg atgacctggc 8101 caagctgctg tggctgaaaa gccccagctc agaggtgtgg tttgaccgaa gaaccaatta 8161 tactcgctcc ctggctgtca tgtccatggt tggatacatt ttaggccttg gagacaggca 8221 cccatccaac ctgatgctgg accggctgag tggaaagatc ctgcacattg agtttgggga 8281 ctgctttgag attgctatga ccagagagaa atttccagaa aagattccat ttagactaac 8341 aagaatgttg accaatgcta tggaggttac cggtctcgat ggcaactata gaaccacatg 8401 ccacacagtg atggaggtgc ttcgggagca caaggacagt gtcatggctg tgctagaagc 8461 ctttgtctat gaccctctgc tgaattggag gctgatggac acaaatgcca aaggcaacaa 8521 gcggtcccga accaggacag actcctattc tgcaggccag tcagtagaaa ttttggacgg 8581 tgtagaactt ggagaaccag cccataagaa aacagggacc actgtgccag aatccatcca 8641 ttctttcatt ggagatggtt tggtgaaacc agaagcctta aacaagaaag ctattcagat 8701 tattaacagg gttcgagata agctcactgg tcgggatttc tctcatgatg acactttgga 8761 tgttccaacc caagtggaac tgcttatcaa gcaagcgaca tctcatgaga acctctgcca 8821 gtgctacatt ggctggtgtc ctttctggta atagtctaga gtcgacctgc agaagcttgg 8881 ccgccatggc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc 8941 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc 9001 atcaatgtat cttatcatgt ctggatcggg aattaattcg gcgcagcacc atggcctgaa 9061 ataacctctg aaagaggaac ttggttaggt accttctgag gcggaaagaa ccagctgtgg 9121 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa 9181 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc 9241 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg 9301 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt 9361 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga 9421 ggaggctttt ttggaggcct aggcttttgc aaaaagctgt taacagcttg gcactggccg 9481 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag 9541 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc 9601 aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc 9661 tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc 9721 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct 9781 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg 9841 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga 9901 ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt 9961 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg 10021 aacaacactc aaccctatct cgggctattc ttttgattta taagggattt tgccgatttc 10081 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat 10141 attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt 10201 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc 10261 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc 10321 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt 10381 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg 10441 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca 10501 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt 10561 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga 10621 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga 10681 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat 10741 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca 10801 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt 10861 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac 10921 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct 10981 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga 11041 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac 11101 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat 11161 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg 11221 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc 11281 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc 11341 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg 11401 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta 11461 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg 11521 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga 11581 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt 11641 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag 11701 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa 11761 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag 11821 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca 11881 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac 11941 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa 12001 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc 12061 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg 12121 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc 12181 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc 12241 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag 12301 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaag //