LOCUS Exported 6416 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS Hsp68-CreER(TM) (DM#264) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6416) TITLE Other Melton Lab plasmids REFERENCE 2 (bases 1 to 6416) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6416 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 107..128 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 143..173 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 181..197 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 186..208 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 205..221 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 205..221 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 240..260 /label=T3 /note="T3 promoter, forward primer" promoter 242..260 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind 301..317 /label=pBluescriptKS /note="For pBluescript vector" primer_bind 302..318 /label=KS primer /note="common sequencing primer, one of multiple similar variants" CDS 1231..2259 /codon_start=1 /product="site-specific recombinase" /label=Cre /note="Cre is a site-specific recombinase from bacteriophage P1. Recombination occurs at loxP sequences." /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE DGD" polyA_signal 3640..3774 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3677..3696) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 3731..3750 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(3846..3865) /label=T7 /note="T7 promoter, forward primer" promoter complement(3847..3865) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(3872..3889) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(3872..3888) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3881..3903) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 4030..4485 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4117..4136) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 4327..4348 /label=F1ori-F /note="F1 origin, forward primer" promoter 4511..4615 /gene="bla" /label=AmpR promoter CDS 4616..5476 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(4834..4853) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 5647..6235 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6136..6155 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 6389..6406 /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 121 tcactcatta ggcaccccag gctttacact ttatgctccc ggctcgtatg ttgtgtggaa 181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg 241 caattaaccc tcactaaagg gaacaaaagc tgggtacggc cggccgtacc gggccccccc 301 tcgaggtcga cggtatcgat aagcttgata tcgaattcct gcagccccgc gggtaccgag 361 ctccaggaac atccaaactg agcagccggg gtccccccca ccccccaccc cgcccctccc 421 ggcaactttg agcctgtgct gggacagagc ctctagttcc taaattagtc catgaggtca 481 gaggcagcac tgccattgta acgcgattgg agaggatcac gtcaccggac acgcccccag 541 gcatctccct gggtctccta aacttggcgg ggagaagttt tagcccttaa gttttagcct 601 ttaaccccca tattcagaac tgtgcgagtt ggcgaaaccc cacaaatcac aacaaactgt 661 acacaacacc gagctagagg tgatctttct tgtccattcc acacaggcct tagtaatgcg 721 tcgccatagc aacagtgtca ctagtagcac cagcacttcc ccacaccctc cccctcagga 781 atccgtactc tccagtgaac cccagaaacc tctggagagt tctggacaag ggcggaaccc 841 acaactccga ttactcaagg gaggcgggga agctccacca gacgcgaaac tgctggaaga 901 ttcctggccc caaggcctcc tccggctcgc tgattggccc agcggagagt gggcggggcc 961 ggtgaagact ccttaaaggc gcagggcggc gagcaggtca ccagacgctg acagctactc 1021 agaaccaaat ctggttccat ccagagacaa gcgaagacaa gagaagcaga gcgagcggcg 1081 cgttcccgat cctcggccag gaccagcctt ccccagagca tccctgccgc ggagcgcaac 1141 cttcccagga gcatccctgc cgcggagcgc aactttcccc ggagcatcca cgccgcggag 1201 cgcagccttc cagaagcaga gcgcggcgcc atgtccaatt tactgaccgt acaccaaaat 1261 ttgcctgcat taccggtcga tgcaacgagt gatgaggttc gcaagaacct gatggacatg 1321 ttcagggatc gccaggcgtt ttctgagcat acctggaaaa tgcttctgtc cgtttgccgg 1381 tcgtgggcgg catggtgcaa gttgaataac cggaaatggt ttcccgcaga acctgaagat 1441 gttcgcgatt atcttctata tcttcaggcg cgcggtctgg cagtaaaaac tatccagcaa 1501 catttgggcc agctaaacat gcttcatcgt cggtccgggc tgccacgacc aagtgacagc 1561 aatgctgttt cactggttat gcggcggatc cgaaaagaaa acgttgatgc cggtgaacgt 1621 gcaaaacagg ctctagcgtt cgaacgcact gatttcgacc aggttcgttc actcatggaa 1681 aatagcgatc gctgccagga tatacgtaat ctggcatttc tggggattgc ttataacacc 1741 ctgttacgta tagccgaaat tgccaggatc agggttaaag atatctcacg tactgacggt 1801 gggagaatgt taatccatat tggcagaacg aaaacgctgg ttagcaccgc aggtgtagag 1861 aaggcactta gcctgggggt aactaaactg gtcgagcgat ggatttccgt ctctggtgta 1921 gctgatgatc cgaataacta cctgttttgc cgggtcagaa aaaatggtgt tgccgcgcca 1981 tctgccacca gccagctatc aactcgcgcc ctggaaggga tttttgaagc aactcatcga 2041 ttgatttacg gcgctaagga tgactctggt cagagatacc tggcctggtc tggacacagt 2101 gcccgtgtcg gagccgcgcg agatatggcc cgcgctggag tttcaatacc ggagatcatg 2161 caagctggtg gctggaccaa tgtaaatatt gtcatgaact atatccgtaa cctggatagt 2221 gaaacagggg caatggtgcg cctgctggaa gatggggatc aggctggtgc catgggcgat 2281 ccacgaaatg aaatgggtgc ttcaggagac atgagggctg ccaacctttg gccaagccct 2341 cttgtgatta agcacactaa gaagaatagc cctgccttgt ccttgacagc tgaccagatg 2401 gtcagtgcct tgttggatgc tgaaccgccc atgatctatt ctgaatatga tccttctaga 2461 cccttcagtg aagcctcaat gatgggctta ttgaccaacc tagcagatag ggagctggtt 2521 catatgatca actgggcaaa gagagtgcca ggctttgggg acttgaatct ccatgatcag 2581 gtccaccttc tcgagtgtgc ctggctggag attctgatga ttggtctcgt ctggcgctcc 2641 atggaacacc cggggaagct cctgtttgct cctaacttgc tcctggacag gaatcaaggt 2701 aaatgtgtgg aaggcatggt ggagatcttt gacatgttgc ttgctacgtc aagtcggttc 2761 cgcatgatga acctgcaggg tgaagagttt gtgtgcctca aatccatcat tttgcttaat 2821 tccggagtgt acacgtttct gtccagcacc ttgaagtctc tggaagagaa ggaccacatc 2881 caccgtgtcc tggacaagat cacagacact ttgatccacc tgatggccaa agctggcctg 2941 actctgcagc agcagcatcg ccgcctagct cagctccttc tcattctttc ccatatccgg 3001 catatgagta acaaacgcat ggagcatctc tacaacatga aatgcaagaa cgtggtaccc 3061 ctctatgacc tgctcctgga gatgttggat gcccaccgcc ttcatgcccc agccagtcgc 3121 atgggagtgc ccccagagga gcccagccag acccagctgg ccaccaccag ctccacttca 3181 gcacattcct tacaaaccta ctacataccc ccggaagcag agggcttccc caacacgatc 3241 tgagagctcc tactagtcta gcttgggatc tttgtgaagg aaccttactt ctgtggtgtg 3301 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta 3361 agtgtataat gtgttaaact agctgcatat gcttgctgct tgagagtttt gcttactgag 3421 tatgatttat gaaaatatta tacacaggag ctagtgattc taattgtttg tgtattttag 3481 attcacagtc ccaaggctca tttcaggccc ctcagtcctc acagtctgtt catgatcata 3541 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc 3601 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat 3661 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg 3721 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcgatctg 3781 cggccgccac cgcggtggag ctgtttaaac agctgtttaa acagctgttt aaacgctcca 3841 attcgcccta tagtgagtcg tattacaatt cactggccgt cgttttacaa cgtcgtgact 3901 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct 3961 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg 4021 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca 4081 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct 4141 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt 4201 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac 4261 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct 4321 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt 4381 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac 4441 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg 4501 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc 4561 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag 4621 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt 4681 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt 4741 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga 4801 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat 4861 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga 4921 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag 4981 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg 5041 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg 5101 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt 5161 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg 5221 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc 5281 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg 5341 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac 5401 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact 5461 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa 5521 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa 5581 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg 5641 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc 5701 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac 5761 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca 5821 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt 5881 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc 5941 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg 6001 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc 6061 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac 6121 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct 6181 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc 6241 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt 6301 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac 6361 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaag //