LOCUS Exported 1017 bp ds-DNA linear SYN 24-MAY-2021 DEFINITION Transcriptional unit of (human codon optimized) inactivated Cas9 fused to the BRD Transcriptional Repressor. ACCESSION . VERSION . KEYWORDS pEGB 35s:dCas:BRD:tNos (GB1172) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1017) AUTHORS Vazquez-Vilar M, Bernabe-Orts JM, Fernandez-Del-Carmen A, Ziarsolo P, Blanca J, Granell A, Orzaez D TITLE A modular toolbox for gRNA-Cas9 genome engineering in plants based on the GoldenBraid standard. JOURNAL Plant Methods. 2016 Feb 1;12:10. doi: 10.1186/s13007-016-0101-2. eCollection 2016. PUBMED 26839579 REFERENCE 2 (bases 1 to 1017) AUTHORS . TITLE Direct Submission JOURNAL Exported May 24, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1017 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 96..116 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" ORIGIN 1 catttcattt ggagaggact ccggtatttt tacaacaatt accacaacaa aacaaacaac 61 aaacaacatt acaatttact attctagtcg aaatgcccaa gaagaagagg aaggtggaca 121 agaagtactc cattgggctc gctatcggca caaacagcgt cggctgggcc gtcattacgg 181 acgagtacaa ggtgccgagc aaaaaattca aagttctggg caataccgat cgccacagca 241 taaagaagaa cctcattggc gccctcctgt tcgactccgg ggaaacggcc gaagccacgc 301 ggctcaaaag aacagcacgg cgcagatata cccgcagaaa gaatcggatc tgctacctgc 361 aggagatctt tagtaatgag atggctaagg tggatgactc tttcttccat aggctggagg 421 agtccttttt ggtggaggag gataaaaagc acgagcgcca cccaatcttt ggcaatatcg 481 tggacgaggt ggcgtaccat gaaaagtacc caaccatata tcatctgagg aagaagcttg 541 tagacagtac tgataaggct gacttgcggt tgatctatct cgcgctggcg catatgatca 601 aatttcgggg acacttcctc atcgaggggg acctgaaccc agacaacagc gatgtcgaca 661 aactctttat ccaactggtt cagacttaca atcagctttt cgaagagaac ccgatcaacg 721 catccggagt tgacgccaaa gcaatcctga gcgctaggct gtccaaatcc cggcggctcg 781 aaaacctcat cgcacagctc cctggggaga agaagaacgg cctgtttggt aatcttatcg 841 ccctgtcact cgggctgacc cccaacttta aatctaactt cgacctggcc gaagatgcca 901 agcttcaact gagcaaagac acctacgatg atgatctcga caatctgctg gcccagatcg 961 gcgaccagta cgcagacctt tttttggcgg caaagaacct gtcagacgcc attctgc //