LOCUS Exported 957 bp ds-DNA linear SYN 18-MAY-2021 DEFINITION 3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Neo. ACCESSION . VERSION . KEYWORDS pLenti CMV Neo DEST (705-1) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 957) AUTHORS Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, Campisi J, Yaswen P, Cooper PK, Kaufman PD. TITLE A versatile viral system for expression and depletion of proteins in mammalian cells. JOURNAL PLoS One. 2009 Aug 6;4(8):e6529. PUBMED 19657394 REFERENCE 2 (bases 1 to 957) AUTHORS . TITLE Direct Submission JOURNAL Exported May 18, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..957 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin 38..173 /label=SV40 ori /note="SV40 origin of replication" primer_bind complement(92..111) /label=SV40pro-F /note="SV40 promoter/origin, forward primer" polyA_signal 213..334 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(224..243) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 278..297 /label=SV40pA-R /note="SV40 polyA, reverse primer" LTR 406..639 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" primer_bind complement(841..860) /label=Neo-F /note="Neomycin resistance gene, forward primer" ORIGIN 1 atacgactca ctatagggcg aattgggtac gtccctaggc ctccaaaaaa gcctcctcac 61 tacttctgga atagctcaga ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt 121 agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg gatgggcgga 181 gttaggggcg ggatagctag agccagacat gataagatac attgatgagt ttggacaaac 241 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt 301 atttgtaacc attataagct gcaataaaca agttcctctc actctctgat attcatttct 361 ttgcaagtta taaatactga ataataagat gacatgaact actactgcta gagattttcc 421 acactgacta aaagggtctg agggatctct agttaccaga gtcacacaac agacgggcac 481 acactacttg aagcactcaa ggcaagcttt attgaggctt aagcagtggg ttccctagtt 541 agccagagag ctcccaggct cagatctggt ctaaccagag agacccagta caagcaaaaa 601 gcagatcttg tcttcgttgg gagtgagcta gcccttccag tccccccttt tcttttaaaa 661 agtggctaag atctacagct gccttgtaag tcattggtct taaaggtacc gggccctcga 721 cggatcccct cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg 781 agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc 841 aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca 901 gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatc //