LOCUS Exported 9805 bp ds-DNA circular SYN 03-MAR-2023 DEFINITION 3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Neo. ACCESSION . VERSION . KEYWORDS pLenti CMV Neo DEST (705-1) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9805) AUTHORS Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, Campisi J, Yaswen P, Cooper PK, Kaufman PD. TITLE A versatile viral system for expression and depletion of proteins in mammalian cells. JOURNAL PLoS One. 2009 Aug 6;4(8):e6529. PUBMED 19657394 REFERENCE 2 (bases 1 to 9805) AUTHORS . TITLE Direct Submission JOURNAL Exported Mar 3, 2023 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9805 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 27..131 /gene="bla" /label=AmpR promoter CDS 132..992 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(350..369) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1163..1751 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1652..1671 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 1905..1922 /label=L4440 /note="L4440 vector, forward primer" protein_bind 2039..2060 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 2075..2105 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2113..2129 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 2118..2140 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 2137..2153 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 2137..2153 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 2172..2192 /label=T3 /note="T3 promoter, forward primer" promoter 2174..2192 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" LTR 2448..2628 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 2672..2797 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 3290..3523 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 3707..3751 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 4012..4129 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" enhancer 4152..4455 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 4456..4659 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 4610..4630 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 4656..4680 /label=LNCX /note="Human CMV promoter, forward primer" protein_bind 4764..4888 /gene="mutant version of attR" /label=attR1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" promoter 4913..4943 /label=lac UV5 promoter /note="E. coli lac promoter with an ""up"" mutation" CDS 4997..5656 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind complement(5063..5082) /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" CDS 5998..6303 /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /label=ccdB /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" primer_bind 6182..6201 /label=ccdB-fwd /note="ccdB gene, forward primer" protein_bind complement(6344..6468) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" misc_feature 6499..7087 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(6552..6572) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(6970..6981) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" promoter 7130..7629 /label=PGK promoter /note="mouse phosphoglycerate kinase 1 promoter" primer_bind complement(7165..7188) /label=MSCV-rev /note="Murine stem cell virus, reverse primer" primer_bind 7562..7581 /label=mPGK-F /note="Mouse PGK promoter, forward primer" CDS 7643..8446 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(7706..7725) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 8316..8335 /label=Neo-F /note="Neomycin resistance gene, forward primer" LTR 8537..8770 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 8842..8963 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(8879..8898) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 8933..8952 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 9003..9138 /label=SV40 ori /note="SV40 origin of replication" primer_bind 9065..9084 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(9158..9177) /label=T7 /note="T7 promoter, forward primer" promoter complement(9159..9177) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(9187..9204) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(9187..9203) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(9196..9218) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 9348..9803 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(9435..9454) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 9645..9666 /label=F1ori-F /note="F1 origin, forward primer" ORIGIN 1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac 61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa 121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat 181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga 301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg 361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc 421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag 481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc 541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg 601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg 661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac 721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac 781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg 841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg 901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg 961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac 1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt 1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct 1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta 2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta 2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt 2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat 2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc 2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg 2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc 2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt 2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc 2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta 2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa 2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga 2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag 2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag 2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca 2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac 2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga 3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga 3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac 3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt 3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca 3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt 3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg 3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta 3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa 3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct 3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc 3601 tggaacagat tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac 3661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga 3721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct 3781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt 3841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac 3901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga 3961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggttaa cttttaaaag 4021 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga 4081 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta tcgataagct 4141 tgggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac 4201 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc 4261 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg 4321 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat 4381 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc 4441 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt 4501 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac 4561 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc 4621 ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc 4681 gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccgact ctagtccagt 4741 gtggtggaat tctgcagata tcaacaagtt tgtacaaaaa agctgaacga gaaacgtaaa 4801 atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact 4861 gtaaaacaca acatatccag tcactatggc ggccgcatta ggcaccccag gctttacact 4921 ttatgcttcc ggctcgtata atgtgtggat tttgagttag gatccggcga gattttcagg 4981 agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca 5041 atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca 5101 gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt 5161 ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat 5221 ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt 5281 ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca 5341 gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc 5401 taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag 5461 ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa 5521 atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt 5581 ctgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg 5641 gcagggcggg gcgtaaagat ctggatccgg cttactaaaa gccagataac agtatgcgta 5701 tttgcgcgct gatttttgcg gtataagaat atatactgat atgtataccc gaagtatgtc 5761 aaaaagaggt gtgctatgaa gcagcgtatt acagtgacag ttgacagcga cagctatcag 5821 ttgctcaagg catatatgat gtcaatatct ccggtctggt aagcacaacc atgcagaatg 5881 aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg atggctgagg 5941 tcgcccggtt tattgaaatg aacggctctt ttgctgacga gaacagggac tggtgaaatg 6001 cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt ggatgtacag 6061 agtgatatta ttgacacgcc cgggcgacgg atggtgatcc ccctggccag tgcacgtctg 6121 ctgtcagata aagtctcccg tgaactttac ccggtggtgc atatcgggga tgaaagctgg 6181 cgcatgatga ccaccgatat ggccagtgtg ccggtctccg ttatcgggga agaagtggct 6241 gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt ctggggaata 6301 taaatgtcag gctccgttat acacagccag tctgcaggtc gaccatagtg actggatatg 6361 ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat 6421 atttatatca ttttacgttt ctcgttcagc tttcttgtac aaagtggttg atatccagca 6481 cagtggcggc cgctcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt 6541 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat 6601 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg 6661 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt 6721 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact 6781 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc 6841 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg 6901 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc 6961 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg 7021 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc 7081 tccccgcctg gaattctgca gatatccgga ctagtgatct aattctaccg ggtaggggag 7141 gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc 7201 tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct 7261 ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc 7321 cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt 7381 ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc 7441 caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg 7501 ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg 7561 gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg 7621 ggcctttcga cctgcagcca atatgggatc ggccattgaa caagatggat tgcacgcagg 7681 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg 7741 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa 7801 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct 7861 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga 7921 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc 7981 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac 8041 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc 8101 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact 8161 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga 8221 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg 8281 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga 8341 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga 8401 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagggg atccgtcgag 8461 ggcccggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta 8521 aaagaaaagg ggggactgga agggctagct cactcccaac gaagacaaga tctgcttttt 8581 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta 8641 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc 8701 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa 8761 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa 8821 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc 8881 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg 8941 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc 9001 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt 9061 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag 9121 gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc 9181 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact 9241 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac 9301 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcgacg cgccctgtag 9361 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag 9421 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt 9481 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca 9541 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata 9601 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca 9661 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc 9721 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa 9781 caaaatatta acgtttacaa tttcc //