LOCUS Exported 9569 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Use with Addgene 40615 for blue light transcription control in yeast . ACCESSION . VERSION . KEYWORDS pDBTrp-LexABD-CRY2FL SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9569) AUTHORS Pathak GP, Strickland D, Vrana JD, Tucker CL TITLE Benchmarking of optical dimerizer systems. JOURNAL ACS Synth Biol. 2014 Nov 21;3(11):832-8. doi: 10.1021/sb500291r. Epub 2014 Nov 5. PUBMED 25350266 REFERENCE 2 (bases 1 to 9569) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9569 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(10..28) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" misc_feature 70..573 /label=CEN/ARS /note="S. cerevisiae CEN6 centromere fused to an autonomously replicating sequence" primer_bind complement(801..820) /label=pENTR-R /note="pENTR vectors, reverse primer" CDS 812..1627 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCWRPRLNSNMDADLYGYKWARDNVGQSGATIFRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIRGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHTLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(885..904) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" rep_origin 1764..2352 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2506..2523 /label=L4440 /note="L4440 vector, forward primer" promoter 2676..2706 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2714..2730 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 2719..2741 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 2738..2754 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" promoter 2775..2793 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" terminator 2824..3071 /gene="S. cerevisiae CYC1" /label=CYC1 terminator /note="transcription terminator for CYC1" primer_bind 3037..3055 /label=CYC1 /note="CYC1 transcription termination signal, reverse primer" promoter 5332..5733 /gene="S. cerevisiae ADH1" /label=ADH1 promoter /note="promoter for alcohol dehydrogenase 1" CDS 5761..6366 /codon_start=1 /gene="lexA" /product="E. coli DNA-binding protein that represses genes involved in the SOS response to DNA damage" /label=LexA /note="The N-terminal DNA-binding domain recognizes the 16-bp palindromic sequence 5'-CTGTATATATATACAG-3'." /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL" primer_bind 6304..6325 /label=LexA /note="LexA DNA binding domain, forward primer" regulatory 6388..6397 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 6460..8298 /codon_start=1 /product="Arabidopsis thaliana cryptochrome 2" /label=CRY2 /note="binds to CIB1 upon exposure to blue light (Kennedy et al., 2010)" /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAAPDEIVADSFEALGANTIKE PGLCPSVSSNDQQVPSAVRYNGSKRVKPEEEEERDMKKSRGFDERELFSTAESSSSSSV FFVSQSCSLASEGKNLEGIQDSSDQITTSLGKNGCK" terminator 8332..8519 /gene="S. cerevisiae ADH1" /label=ADH1 terminator /note="transcription terminator for the S. cerevisiae alcohol dehydrogenase 1 (ADH1) gene" primer_bind complement(8387..8411) /label=pHybLex-R /note="ADH terminator, reverse primer" CDS complement(8572..9246) /codon_start=1 /gene="S. cerevisiae TRP1" /product="phosphoribosylanthranilate isomerase, required for tryptophan biosynthesis" /label=TRP1 /note="yeast auxotrophic marker" /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA KK" promoter complement(9247..9348) /gene="S. cerevisiae TRP1" /label=TRP1 promoter primer_bind complement(9363..9379) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(9400..9419) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 9519..9541 /label=pGEX 3' /note="pGEX vectors, reverse primer" ORIGIN 1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 61 cttaggacgg atcgcttgcc tgtaacttac acgcgcctcg tatcttttaa tgatggaata 121 atttgggaat ttactctgtg tttatttatt tttatgtttt gtatttggat tttagaaagt 181 aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa aataaacaaa ggtttaaaaa 241 atttcaacaa aaagcgtact ttacatatat atttattaga caagaaaagc agattaaata 301 gatatacatt cgattaacga taagtaaaat gtaaaatcac aggattttcg tgtgtggtct 361 tctacacaga caagatgaaa caattcggca ttaatacctg agagcaggaa gagcaagata 421 aaaggtagta tttgttggcg atccccctag agtcttttac atcttcggaa aacaaaaact 481 attttttctt taatttcttt ttttactttc tatttttaat ttatatattt atattaaaaa 541 atttaaatta taattatttt tatagcacgt gatgaaaagg acccaggtgg cacttttcgg 601 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg 661 ctcatgagac aataaccctg ataaatgctt caataatctg cagctctggc ccgtgtctca 721 aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc 781 tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcttg 841 ctggaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg 901 cgataatgtc gggcaatcag gtgcgacaat ctttcgattg tatgggaagc ccgatgcgcc 961 agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt 1021 cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac 1081 tcctgatgat gcatggttac tcaccactgc gatccgcggg aaaacagcat tccaggtatt 1141 agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg 1201 gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc 1261 tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg 1321 taatggctgg cctgttgaac aagtctggaa agaaatgcat acgcttttgc cattctcacc 1381 ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa 1441 attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc 1501 catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa 1561 atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt 1621 tttctaatca gaattggtta attggttgta acactggcag agcattacgc tgacttgacg 1681 ggacggcgca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc 1741 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg 1801 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact 1861 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg 1921 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg 1981 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac 2041 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca 2101 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga 2161 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc 2221 ggaacaggag agcgcacgag ggagcttcca ggggggaacg cctggtatct ttatagtcct 2281 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggccg 2341 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct 2401 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc 2461 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc 2521 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat 2581 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt 2641 aatgtgagtt acctcactca ttaggcaccc caggctttac actttatgct tccggctcct 2701 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat 2761 tacgccaagc tcggaattaa ccctcactaa agggaacaaa agctggtacc gatcccgagc 2821 tttgcaaatt aaagccttcg agcgtcccaa aaccttctca agcaaggttt tcagtataat 2881 gttacatgcg tacacgcgtc tgtacagaaa aaaaagaaaa atttgaaata taaataacgt 2941 tcttaatact aacataacta taaaaaaata aatagggacc tagacttcag gttgtctaac 3001 tccttccttt tcggttagag cggatgtggg gggagggcgt gaatgtaagc gtgacataac 3061 taattacatg atatcgacaa aggaaaaggg gcctgtttac tcacaggctt ttttcaagta 3121 ggtaattaag tcgtttctgt ctttttcctt cttcaaccca ccaaaggcca tcttggtact 3181 tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt 3241 tttttttttt tttttttttt tttttttttt tttcatagaa ataatacaga agtagatgtt 3301 gaattagatt aaactgaaga tatataattt attggaaaat acatagagct ttttgttgat 3361 gcgcttaagc gatcaattca acaacaccac cagcagctct gattttttct tcagccaact 3421 tggagacgaa tctagctttg acgataactg gaacatttgg aattctaccc ttacccaaga 3481 tcttaccgta accggctgcc aaagtgtcaa taactggagc agtttcctta gaagcagatt 3541 tcaagtattg gtctctcttg tcttctggga tcaatgtcca caatttgtcc aagttcaaga 3601 ctggcttcca gaaatgagct tgttgcttgt ggaagtatct cataccaacc ttaccgaaat 3661 aacctggatg gtatttatcc atgttaattc tgtggtgatg ttgaccaccg gccatacctc 3721 taccaccggg gtgctttctg tgcttaccga tacgaccttt accggctgag acgtgacctc 3781 tgtgctttct agtcttagtg aatctggaag gcattcttga ttagttggat gattgttctg 3841 ggatttaatg caaaaatcac ttaagaagga aaatcaacgg agaaagcaaa cgccatctta 3901 aatatacggg atacagatga aagggtttga acctatctgg aaaatagcat taaacaagcg 3961 aaaaactgcg aggaaaattg tttgcgtctc tgcgggctat tcacgcgcca gaggaaaata 4021 ggaaaaataa cagggcatta gaaaaataat tttgattttg gtaatgtgtg ggtcctggtg 4081 tacagatgtt acattggtta cagtactctt gtttttgctg tgtttttcga tgaatctcca 4141 aaatggttgt tagcacatgg aagagtcacc gatgctaagt tatctctatg taagctacgt 4201 ggcgtgactt ttgatgaagc cgcacaagag atacaggatt ggcaactgca aatagaatct 4261 ggggatcccc cctcgagatc cgggatcgaa gaaatgatgg taaatgaaat aggaaatcaa 4321 ggagcatgaa ggcaaaagac aaatataagg gtcgaacgaa aaataaagtg aaaagtgttg 4381 atatgatgta tttggctttg cggcgccgaa aaaacgagtt tacgcaattg cacaatcatg 4441 ctgactctgt ggcggacccg cgctcttgcc ggcccggcga taacgctggg cgtgaggctg 4501 tgcccggcgg agttttttgc gcctgcattt tccaaggttt accctgcgct aaggggcgag 4561 attggagaag caataagaat gccggttggg gttgcgatga tgacgaccac gacaactggt 4621 gtcattattt aagttgccga aagaacctga gtgcatttgc aacatgagta tactagaaga 4681 atgagccaag acttgcgaga cgcgagtttg ccggtggtgc gaacaataga gcgaccatga 4741 ccttgaaggt gagacgcgca taaccgctag agtactttga agaggaaaca gcaatagggt 4801 tgctaccagt ataaatagac aggtacatac aacactggaa atggttgtct gtttgagtac 4861 gctttcaatt catttgggtg tgcactttat tatgttacaa tatggaaggg aactttacac 4921 ttctcctatg cacatatatt aattaaagtc caatgctagt agagaagggg ggtaacaccc 4981 ctccgcgctc ttttccgatt tttttctaaa ccgtggaata tttcggatat ccttttgttg 5041 tttccgggtg tacaatatgg acttcctctt ttctggcaac caaacccata catcgggatt 5101 cctataatac cttcgttggt ctccctaaca tgtaggtggc ggaggggaga tatacaatag 5161 aacagatacc agacaagaca taatgggcta aacaagacta caccaattac actgcctcat 5221 tgatggtggt acataacgaa ctaatactgt agccctagac ttgatagcca tcatcatatc 5281 gaagtttcac tacccttttt ccatttgcca tctattgaag taataatagg cgcatgcaac 5341 ttcttttctt tttttttctt ttctctctcc cccgttgttg tctcaccata tccgcaatga 5401 caaaaaaaat gatggaagac actaaaggaa aaaattaacg acaaagacag caccaacaga 5461 tgtcgttgtt ccagagctga tgaggggtat cttcgaacac acgaaacttt ttccttcctt 5521 cattcacgca cactactctc taatgagcaa cggtatacgg ccttccttcc agttacttga 5581 atttgaaata aaaaaagttt gccgctttgc tatcaagtat aaatagacct gcaattatta 5641 atcttttgtt tcctcgtcat tgttctcgtt ccctttcttc cttgtttctt tttctgcaca 5701 atatttcaag ctataccaag catacaatca actccaagct tgaagcaagc ctcctgaaag 5761 atgaaagcgt taacggccag gcaacaagag gtgtttgatc tcatccgtga tcacatcagc 5821 cagacaggta tgccgccgac gcgtgcggaa atcgcgcagc gtttggggtt ccgttcccca 5881 aacgcggctg aagaacatct gaaggcgctg gcacgcaaag gcgttattga aattgtttcc 5941 ggcgcatcac gcgggattcg tctgttgcag gaagaggaag aagggttgcc gctggtaggt 6001 cgtgtggctg ccggtgaacc acttctggcg caacagcata ttgaaggtca ttatcaggtc 6061 gatccttcct tattcaagcc gaatgctgat ttcctgctgc gcgtcagcgg gatgtcgatg 6121 aaagatatcg gcattatgga tggtgacttg ctggcagtgc ataaaactca ggatgtacgt 6181 aacggtcagg tcgttgtcgc acgtattgat gacgaggtta ccgttaagcg cctgaaaaaa 6241 cagggcaata aagtcgaact gttgccagaa aatagcgagt ttaaaccaat tgtcgtagat 6301 cttcgtcagc agagcttcac cattgaaggg ctggcggttg gggttattcg caacggcgac 6361 tggctggaat tcccggggat ccgtcgacca tggcggccgc tcgagtcgac ctgcagccaa 6421 gctaattccg ggcgaatttc ttatgattta ggcggcagca tgaagatgga caaaaagact 6481 atagtttggt ttagaagaga cctaaggatt gaggataatc ctgcattagc agcagctgct 6541 cacgaaggat ctgtttttcc tgtcttcatt tggtgtcctg aagaagaagg acagttttat 6601 cctggaagag cttcaagatg gtggatgaaa caatcacttg ctcacttatc tcaatccttg 6661 aaggctcttg gatctgacct cactttaatc aaaacccaca acacgatttc agcgatcttg 6721 gattgtatcc gcgttaccgg tgctacaaaa gtcgtcttta accacctcta tgatcctgtt 6781 tcgttagttc gggaccatac cgtaaaggag aagctggtgg aacgtgggat ctctgtgcaa 6841 agctacaatg gagatctatt gtatgaaccg tgggagatat actgcgaaaa gggcaaacct 6901 tttacgagtt tcaattctta ctggaagaaa tgcttagata tgtcgattga atccgttatg 6961 cttcctcctc cttggcggtt gatgccaata actgcagcgg ctgaagcgat ttgggcgtgt 7021 tcgattgaag aactagggct ggagaatgag gccgagaaac cgagcaatgc gttgttaact 7081 agagcttggt ctccaggatg gagcaatgct gataagttac taaatgagtt catcgagaag 7141 cagttgatag attatgcaaa gaacagcaag aaagttgttg ggaattctac ttcactactt 7201 tctccgtatc tccatttcgg ggaaataagc gtcagacacg ttttccagtg tgcccggatg 7261 aaacaaatta tatgggcaag agataagaac agtgaaggag aagaaagtgc agatcttttt 7321 cttaggggaa tcggtttaag agagtattct cggtatatat gtttcaactt cccgtttact 7381 cacgagcaat cgttgttgag tcatcttcgg tttttccctt gggatgctga tgttgataag 7441 ttcaaggcct ggagacaagg caggaccggt tatccgttgg tggatgccgg aatgagagag 7501 ctttgggcta ccggatggat gcataacaga ataagagtga ttgtttcaag ctttgctgtg 7561 aagtttcttc tccttccatg gaaatgggga atgaagtatt tctgggatac acttttggat 7621 gctgatttgg aatgtgacat ccttggctgg cagtatatct ctgggagtat ccccgatggc 7681 cacgagcttg atcgcttgga caatcccgcg ttacaaggcg ccaaatatga cccagaaggt 7741 gagtacataa ggcaatggct tcccgagctt gcgagattgc caactgaatg gatccatcat 7801 ccatgggacg ctcctttaac cgtactcaaa gcttctggtg tggaactcgg aacaaactat 7861 gcgaaaccca ttgtagacat cgacacagct cgtgagctac tagctaaagc tatttcaaga 7921 acccgtgaag cacagatcat gatcggagca gcacctgatg agattgtagc agatagcttc 7981 gaggccttag gggctaatac cattaaagaa cctggtcttt gcccatctgt gtcttctaat 8041 gaccaacaag taccttcggc tgttcgttac aacgggtcaa agagagtgaa acctgaggaa 8101 gaagaagaga gagacatgaa gaaatctagg ggattcgatg aaagggagtt gttttcgact 8161 gctgaatctt cttcttcttc gagtgtgttt ttcgtttcgc agtcttgctc gttggcatca 8221 gaagggaaga atctggaagg tattcaagat tcatctgatc agattactac aagtttggga 8281 aaaaatggtt gcaaatgagg atccgtcgac ctgcagccaa gctaattccg ggcgaatttc 8341 ttatgattta tgatttttat tattaaataa gttataaaaa aaataagtgt atacaaattt 8401 taaagtgact cttaggtttt aaaacgaaaa ttcttgttct tgagtaactc tttcctgtag 8461 gtcaggttgc tttctcaggt atagcatgag gtcgctctta ttgaccacac ctctaccggc 8521 atgccggcaa gtgcacaaac aatacttaaa taaatactac tcagtaataa cctatttctt 8581 agcatttttg acgaaatttg ctattttgtt agagtctttt acaccatttg tctccacacc 8641 tccgcttaca tcaacaccaa taacgccatt taatctaagc gcatcaccaa cattttctgg 8701 cgtcagtcca ccagctaaca taaaatgtaa gctttcgggg ctctcttgcc ttccaaccca 8761 gtcagaaatc gagttccaat ccaaaagttc acctgtccca cctgcttctg aatcaaacaa 8821 gggaataaac gaatgaggtt tctgtgaagc tgcactgagt agtatgttgc agtcttttgg 8881 aaatacgagt cttttaataa ctggcaaacc gaggaactct tggtattctt gccacgactc 8941 atctccatgc agttggacga tatcaatgcc gtaatcattg accagagcca aaacatcctc 9001 cttaggttga ttacgaaaca cgccaaccaa gtatttcgga gtgcctgaac tatttttata 9061 tgcttttaca agacttgaaa ttttccttgc aataaccggg tcaattgttc tctttctatt 9121 gggcacacat ataataccca gcaagtcagc atcggaatct agagcacatt ctgcggcctc 9181 tgtgctctgc aagccgcaaa ctttcaccaa tggaccagaa ctacctgtga aattaataac 9241 agacatactc caagctgcct ttgtgtgctt aatcacgtat actcacgtgc tcaatagtca 9301 ccaatgccct ccctcttggc cctctccttt tcttttttcg accgaattaa ttcgtaatca 9361 tggtcatagc tgtttcctgt cgatatggtg cactctcagt acaatctgct ctgatgccgc 9421 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct 9481 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag 9541 gttttcaccg tcatcaccga aacgcgcga //