LOCUS       Exported               10021 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Cas9, TagBFP, and sgRNA expression.
ACCESSION   .
VERSION     .
KEYWORDS    pCAS9-BFP empty
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10021)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 10021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10021
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             8..477
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             938..1354
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1322..1338
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        1365..1605
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     primer_bind     1365..1385
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     1536..1555
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     enhancer        1712..2091
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2092..2295
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2246..2266
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2350..3048
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             3055..3108
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3109..7212
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7225..7245
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     LTR             7302..7895
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(8096..8113)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8267..8855)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8347..8366)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9033..9893)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9656..9675
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9894..9998)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
       61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
      121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
      181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
      241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
      301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
      361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
      421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
      481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
      541 taagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta
      601 tgcgcctgcg tcggtactag ctagttagct aactagctct gtatctggcg gacccgtggt
      661 ggaactgacg agttctgaac acccggccgc aaccctggga gacgtcccag ggactttggg
      721 ggccgttttt gtggcccgac ctgaggaagg gagtcgatgt ggaatccgac cccgtcagga
      781 tatgtggttc tggtaggaga cgagaaccta aaacagttcc cgcctccgtc tgaatttttg
      841 ctttcggttt ggaaccgaag ccgcgcgtct tgtctgctgc agcatcgttc tgtgttgtct
      901 ctgtctgact gtgtttctgt atttgtctga aaattagggc cagactgtta ccactccctt
      961 aagtttgacc ttagatcact ggaaagatgt cgagcggctc gctcacaacc agtcggtaga
     1021 tgtcaagaag agacgttggg ttaccttctg ctctgcagaa tggccaacct ttaacgtcgg
     1081 atggccgcga gacggcacct ttaaccgaga cctcatcacc caggttaaga tcaaggtctt
     1141 ttcacctggc ccgcatggac acccagacca ggtcccctac atcgtgacct gggaagcctt
     1201 ggcttttgac ccccctccct gggtcaagcc ctttgtacac cctaagcctc cgcctcctct
     1261 tcttccatcc gcgccgtctc tcccccttga acctcctctt tcgaccccgc ctcaatcctc
     1321 cctttatcca gccctcactc cttctctagg cgccggccgg atccgagggc ctatttccca
     1381 tgattccttc atatttgcat atacgataca aggctgttag agagataatt ggaattaatt
     1441 tgactgtaaa cacaaagata ttagtacaaa atacgtgacg tagaaagtaa taatttcttg
     1501 ggtagtttgc agttttaaaa ttatgtttta aaatggacta tcatatgctt accgtaactt
     1561 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaaac accgggccct
     1621 cgatatcgac tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg ctttttttcg
     1681 aattccgatg tacgggccag atatacgcgt tgacattgat tattgtctag ttattaatag
     1741 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
     1801 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
     1861 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
     1921 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
     1981 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
     2041 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
     2101 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
     2161 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
     2221 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
     2281 tatataagca gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt
     2341 aagcttccca tgagcgagct gattaaggag aacatgcaca tgaagctgta catggagggc
     2401 accgtggaca accatcactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc
     2461 acccagacca tgagaatcaa ggtggtcgag ggcggccctc tccccttcgc cttcgacatc
     2521 ctggctacta gcttcctcta cggcagcaag accttcatca accacaccca gggcatcccc
     2581 gacttcttca agcagtcctt ccctgagggc ttcacatggg agagagtcac cacatacgaa
     2641 gacgggggcg tgctgaccgc tacccaggac accagcctcc aggacggctg cctcatctac
     2701 aacgtcaaga tcagaggggt gaacttcaca tccaacggcc ctgtgatgca gaagaaaaca
     2761 ctcggctggg aggccttcac cgagacgctg taccccgctg acggcggcct ggaaggcaga
     2821 aacgacatgg ccctgaagct cgtgggcggg agccatctga tcgcaaacat caagaccaca
     2881 tatagatcca agaaacccgc taagaacctc aagatgcctg gcgtctacta tgtggactac
     2941 agactggaaa gaatcaagga ggccaacaac gagacctacg tcgagcagca cgaggtggca
     3001 gtggccagat actgcgacct ccctagcaaa ctggggcaca agctaaattc cggagagggc
     3061 agaggaagtc tgctaacatg cggtgacgtc gaggagaatc ctggcccaat ggacaagaag
     3121 tacagcatcg gcctggacat cggcaccaac agcgtgggct gggccgtgat caccgacgag
     3181 tacaaggtgc ccagcaagaa gttcaaggtg ctgggcaaca ccgaccgcca cagcatcaag
     3241 aagaacctga tcggcgccct gctgttcgac agcggcgaga ccgccgaggc cacccgcctg
     3301 aagcgcaccg cccgccgccg ctacacccgc cgcaagaacc gcatctgcta cctgcaggag
     3361 atcttcagca acgagatggc caaggtggac gacagcttct tccaccgcct ggaggagagc
     3421 ttcctggtgg aggaggacaa gaagcacgag cgccacccca tcttcggcaa catcgtggac
     3481 gaggtggcct accacgagaa gtaccccacc atctaccacc tgcgcaagaa gctggtggac
     3541 agcaccgaca aggccgacct gcgcctgatc tacctggccc tggcccacat gatcaagttc
     3601 cgcggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt ggacaagctg
     3661 ttcatccagc tggtgcagac ctacaaccag ctgttcgagg agaaccccat caacgccagc
     3721 ggcgtggacg ccaaggccat cctgagcgcc cgcctgagca agagccgccg cctggagaac
     3781 ctgatcgccc agctgcccgg cgagaagaag aacggcctgt tcggcaacct gatcgccctg
     3841 agcctgggcc tgacccccaa cttcaagagc aacttcgacc tggccgagga cgccaagctg
     3901 cagctgagca aggacaccta cgacgacgac ctggacaacc tgctggccca gatcggcgac
     3961 cagtacgccg acctgttcct ggccgccaag aacctgagcg acgccatcct gctgagcgac
     4021 atcctgcgcg tgaacaccga gatcaccaag gcccccctga gcgccagcat gatcaagcgc
     4081 tacgacgagc accaccagga cctgaccctg ctgaaggccc tggtgcgcca gcagctgccc
     4141 gagaagtaca aggagatctt cttcgaccag agcaagaacg gctacgccgg ctacatcgac
     4201 ggcggcgcca gccaggagga gttctacaag ttcatcaagc ccatcctgga gaagatggac
     4261 ggcaccgagg agctgctggt gaagctgaac cgcgaggacc tgctgcgcaa gcagcgcacc
     4321 ttcgacaacg gcagcatccc ccaccagatc cacctgggcg agctgcacgc catcctgcgc
     4381 cgccaggagg acttctaccc cttcctgaag gacaaccgcg agaagatcga gaagatcctg
     4441 accttccgca tcccctacta cgtcggcccc ctggcccgcg gcaacagccg cttcgcctgg
     4501 atgacccgca agagcgagga gaccatcacc ccctggaact tcgaggaggt ggtggacaag
     4561 ggcgccagcg cccagagctt catcgagcgc atgaccaact tcgacaagaa cctgcccaac
     4621 gagaaggtgc tgcccaagca cagcctgctg tacgagtact tcaccgtgta caacgagctg
     4681 accaaggtga agtacgtgac cgagggcatg cgcaagcccg ccttcctgag cggcgagcag
     4741 aagaaggcca tcgtggacct gctgttcaag accaaccgca aggtgaccgt gaagcagctg
     4801 aaggaggact acttcaagaa gatcgagtgc ttcgacagcg tggagatcag cggcgtggag
     4861 gaccgcttca acgccagcct gggcacctac cacgacctgc tgaagatcat caaggacaag
     4921 gacttcctgg acaacgagga gaacgaggac atcctggagg acatcgtgct gaccctgacc
     4981 ctgttcgagg accgcgagat gatcgaggag cgcctgaaga cctacgccca cctgttcgac
     5041 gacaaggtga tgaagcagct gaagcgccgc cgctacaccg gctggggccg cctgagccgc
     5101 aagctgatca acggcatccg cgacaagcag agcggcaaga ccatcctgga cttcctgaag
     5161 agcgacggct tcgccaaccg caacttcatg cagctgatcc acgacgacag cctgaccttc
     5221 aaggaggaca tccagaaggc ccaggtgagc ggccagggcg acagcctgca cgagcacatc
     5281 gccaacctgg ccggcagccc cgccatcaag aagggcatcc tgcagaccgt gaaggtggtg
     5341 gacgagctgg tgaaggtgat gggccgccac aagcccgaga acatcgtgat cgagatggcc
     5401 cgcgagaacc agaccaccca gaagggccag aagaacagcc gcgagcgcat gaagcgcatc
     5461 gaggagggca tcaaggagct gggcagccag atcctgaagg agcaccccgt ggagaacacc
     5521 cagctgcaga acgagaagct gtacctgtac tacctgcaga acggccgcga catgtacgtg
     5581 gaccaggagc tggacatcaa ccgcctgagc gactacgacg tggaccacat cgtgccccag
     5641 agcttcctga aggacgacag catcgacaac aaggtgctga cccgcagcga caagaaccgc
     5701 ggcaagagcg acaacgtgcc cagcgaggag gtggtgaaga agatgaagaa ctactggcgc
     5761 cagctgctga acgccaagct gatcacccag cgcaagttcg acaacctgac caaggccgag
     5821 cgcggcggcc tgagcgagct ggacaaggcc ggcttcatca agcgccagct ggtggagacc
     5881 cgccagatca ccaagcacgt ggcccagatc ctggacagcc gcatgaacac caagtacgac
     5941 gagaacgaca agctgatccg cgaggtgaag gtgatcaccc tgaagagcaa gctggtgagc
     6001 gacttccgca aggacttcca gttctacaag gtgcgcgaga tcaacaacta ccaccacgcc
     6061 cacgacgcct acctgaacgc cgtggtgggc accgccctga tcaagaagta ccccaagctg
     6121 gagagcgagt tcgtgtacgg cgactacaag gtgtacgacg tgcgcaagat gatcgccaag
     6181 agcgagcagg agatcggcaa ggccaccgcc aagtacttct tctacagcaa catcatgaac
     6241 ttcttcaaga ccgagatcac cctggccaac ggcgagatcc gcaagcgccc cctgatcgag
     6301 accaacggcg agaccggcga gatcgtgtgg gacaagggcc gcgacttcgc caccgtgcgc
     6361 aaggtgctga gcatgcccca ggtgaacatc gtgaagaaga ccgaggtgca gaccggcggc
     6421 ttcagcaagg agagcatcct gcccaagcgc aacagcgaca agctgatcgc ccgcaagaag
     6481 gactgggacc ccaagaagta cggcggcttc gacagcccca ccgtggccta cagcgtgctg
     6541 gtggtggcca aggtggagaa gggcaagagc aagaagctga agagcgtgaa ggagctgctg
     6601 ggcatcacca tcatggagcg cagcagcttc gagaagaacc ccatcgactt cctggaggcc
     6661 aagggctaca aggaggtgaa gaaggacctg atcatcaagc tgcccaagta cagcctgttc
     6721 gagctggaga acggccgcaa gcgcatgctg gccagcgccg gcgagctgca gaagggcaac
     6781 gagctggccc tgcccagcaa gtacgtgaac ttcctgtacc tggccagcca ctacgagaag
     6841 ctgaagggca gccccgagga caacgagcag aagcagctgt tcgtggagca gcacaagcac
     6901 tacctggacg agatcatcga gcagatcagc gagttcagca agcgcgtgat cctggccgac
     6961 gccaacctgg acaaggtgct gagcgcctac aacaagcacc gcgacaagcc catccgcgag
     7021 caggccgaga acatcatcca cctgttcacc ctgaccaacc tgggcgcccc cgccgccttc
     7081 aagtacttcg acaccaccat cgaccgcaag cgctacacca gcaccaagga ggtgctggac
     7141 gccaccctga tccaccagag catcaccggc ctgtacgaga cccgcatcga cctgagccag
     7201 ctgggcggcg acagccgcgc cgaccccaag aagaagcgca aggtgtgagc ggccgcatcg
     7261 ataaaataaa agattttatt tagtctccag aaaaaggggg gaatgaaaga ccccacctgt
     7321 aggtttggca agctagctta agtaacgcca ttttgcaagg catggaaaaa tacataactg
     7381 agaatagaga agttcagatc aaggtcagga acagatggaa cagctgaata tgggccaaac
     7441 aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggaacagct
     7501 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
     7561 acagatggtc cccagatgcg gtccagccct cagcagtttc tagagaacca tcagatgttt
     7621 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
     7681 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
     7741 ctcactcggg gcgccagtcc tccgattgac tgagtcgccc gggtacccgt gtatccaata
     7801 aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg
     7861 agtgattgac tacccgtcag cgggggtctt tcatttccga cttgtggtct cgctgccttg
     7921 ggagggtctc ctctgagtga ttgactaccc gtcagcgggg gtcttcacat gcagcatgta
     7981 tcaaaattaa tttggttttt tttcttaagt atttacatta aatggccata gttgcattaa
     8041 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg gcgctcttcc gcttcctcgc
     8101 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     8161 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     8221 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     8281 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     8341 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     8401 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     8461 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     8521 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     8581 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     8641 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     8701 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     8761 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     8821 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     8881 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     8941 aaaggatctt cacctagatc cttttaaatt aaaaatgaag tttgcggccg caaatcaatc
     9001 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     9061 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     9121 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     9181 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     9241 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     9301 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     9361 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     9421 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     9481 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     9541 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     9601 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat
     9661 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     9721 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     9781 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     9841 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     9901 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     9961 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    10021 c
//
