LOCUS Exported 5514 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION LC-Myosin labeling with near-infrared fluorescent protein miRFP703. ACCESSION . VERSION . KEYWORDS pmiRFP703-Myosin SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5514) AUTHORS Shcherbakova DM, Baloban M, Emelyanov AV, Brenowitz M, Guo P, Verkhusha VV TITLE Bright monomeric near-infrared fluorescent proteins as tags and biosensors for multiscale imaging. JOURNAL Nat Commun. 2016 Aug 19;7:12405. doi: 10.1038/ncomms12405. PUBMED 27539380 REFERENCE 2 (bases 1 to 5514) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5514 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 61..364 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 365..568 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 519..539 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 601..610 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 607..1551 /codon_start=1 /product="bright monomeric near-infrared fluorescent protein engineered from a bacterial phytochrome, with an emission maximum of 703 nm (Shcherbakova et al., 2016)" /label=miRFP703 /label=ORF frame 1 /translation="MVAGHASGSPAFGTASHSNCEHEEIHLAGSIQPHGALLVVSEHDH RVIQASANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGIPVAVRCRIGNPSTE YCGLMHRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTG YDRVMVYRFDEQGHGLVFSECHVPGLESYFGNRYPSSLVPQMARQLYVRQRVRVLVDVT YQPVPLEPRLSPLTGRDLDMSGCFLRSMSPIHLQFLKDMGVRATLAVSLVVGGKLWGLV VCHHYLPRFIRFELRAICKRLAERIATRITALES" polyA_signal 2302..2423 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2339..2358) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 2393..2412 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(2430..2885) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2567..2588) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 2779..2798 /label=F1ori-R /note="F1 origin, reverse primer" promoter 2912..3016 /gene="bla" /label=AmpR promoter promoter 3018..3375 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(3041..3061) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 3226..3361 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3288..3307 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 3410..4204 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(3464..3483) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 4074..4093 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(4392..4411) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 4436..4483 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 4812..5400 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5301..5320 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcggtc 601 gccaccatgg tagcaggtca tgcctctggc agccccgcat tcgggaccgc ctctcattcg 661 aattgcgaac atgaagagat ccacctcgcc ggctcgatcc agccgcatgg cgcgcttctg 721 gtcgtcagcg aacatgatca tcgcgtcatc caggccagcg ccaacgccgc ggaatttctg 781 aatctcggaa gcgtactcgg cgttccgctc gccgagatcg acggcgatct gttgatcaag 841 atcctgccgc atctcgatcc caccgccgaa ggcatcccgg tcgcggtgcg ctgccggatc 901 ggcaatccct ctacggagta ctgcggtctg atgcatcggc ctccggaagg cgggctgatc 961 atcgaactcg aacgtgccgg cccgtcgatc gatctgtcag gcacgctggc gccggcgctg 1021 gagcggatcc gcacggcggg ttcactgcgc gcgctgtgcg atgacaccgt gctgctgttt 1081 cagcagtgca ccggctacga ccgggtgatg gtgtatcgtt tcgatgagca aggccacggc 1141 ctggtattct ccgagtgcca tgtgcctggg ctcgaatcct atttcggcaa ccgctatccg 1201 tcgtcgctgg tcccgcagat ggcgcggcag ctgtacgtgc ggcagcgcgt ccgcgtgctg 1261 gtcgacgtca cctatcagcc ggtgccgctg gagccgcggc tgtcgccgct gaccgggcgc 1321 gatctcgaca tgtcgggctg cttcctgcgc tcgatgtcgc cgatccatct gcagttcctg 1381 aaggacatgg gcgtgcgcgc caccctggcg gtgtcgctgg tggtcggcgg caagctgtgg 1441 ggcctggttg tctgtcacca ttatctgccg cgcttcatcc gtttcgagct gcgggcgatc 1501 tgcaaacggc tcgccgaaag gatcgcgacg cggatcaccg cgcttgagag cggaggtggt 1561 ggatcaggtg gaggaggttc tggaggtggt ggatcaggag gaggtggttc tggaggtggt 1621 ggatcaggag gaagatctcg agctcaagct tcgatgtcga gcaagagagc caaggccaag 1681 accaccaaga agaggcccca gagggctacg tccaatgtct tcgccatgtt tgaccagtcc 1741 cagatccagg agtttaagga ggccttcaac atgattgatc agaaccgcga tggcttcatt 1801 gataaggagg acctgcacga catgttggcc tctctgggga agaaccccac agacgagtac 1861 ctggagggca tgatgaacga ggcgccaggg cccatcaact tcaccatgtt cctcacaatg 1921 tttggggaga agctgaacgg cacagacccc gaggatgtga tccgcaatgc ctttgcctgc 1981 tttgatgagg aggcctcagg cttcatccac gaggaccacc tgagggagct gctcaccacc 2041 atgggcgacc gattcacgga tgaggaggtg gacgagatgt accgcgaggc acccattgat 2101 aagaagggca acttcaacta cgtggagttc actcgcatcc tcaaacacgg cgccaaggac 2161 aaggacgact aaggatccac cggatctaga taactgatca taatcagcca taccacattt 2221 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa 2281 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc 2341 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg 2401 tccaaactca tcaatgtatc ttaacgcgta aattgtaagc gttaatattt tgttaaaatt 2461 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat 2521 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa 2581 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg 2641 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa 2701 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc 2761 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag 2821 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg 2881 cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta 2941 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata 3001 ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg 3061 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag 3121 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg 3181 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact 3241 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag 3301 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc 3361 ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca tgattgaaca 3421 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg 3481 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg 3541 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc 3601 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt 3661 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc 3721 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca 3781 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc 3841 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg 3901 gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct 3961 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc 4021 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc 4081 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta 4141 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt 4201 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga 4261 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac 4321 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccctagg 4381 gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca 4441 ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg 4501 tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc 4561 gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc 4621 aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact ttagattgat 4681 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg 4741 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc 4801 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa 4861 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag 4921 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta 4981 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta 5041 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag 5101 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg 5161 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg 5221 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag 5281 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc 5341 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa 5401 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg 5461 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat gcat //