LOCUS Exported 920 bp ds-DNA linear SYN 14-JUN-2021 DEFINITION empty vector (lacZ) for 2nd generation recipient assembly. ACCESSION . VERSION . KEYWORDS pJOG292 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 920) AUTHORS Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, Boch J, Stuttmann J TITLE Generation of chromosomal deletions in dicotyledonous plants employing a user-friendly genome editing toolkit. JOURNAL Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319. PUBMED 27579989 REFERENCE 2 (bases 1 to 920) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 14, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..920 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 27..51 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" CDS complement(233..556) /codon_start=1 /gene="lacZ fragment" /product="LacZ-alpha fragment of beta-galactosidase" /label=lacZ-alpha /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS DAA" primer_bind 238..257 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 451..473 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 465..482 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 466..482 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" misc_feature 483..539 /label=MCS /note="pUC18/19 multiple cloning site" primer_bind complement(552..568) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(552..568) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(565..587) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 576..592 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(600..630) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 645..666 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." misc_feature 771..795 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA" ORIGIN 1 ttctaataaa cgctcttttc tcttaggttt acccgccaat atatcctgtc aaacactgat 61 agtttaaact gaaggcggga aacgacaatc tgatccaagc tcaagctaag ctcgacgtcg 121 catgcctgca gaagcttgtc ttcgtcacag cttgtctgta agcggatgcc gggagcagac 181 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg 241 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg 301 taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag 361 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa 421 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca 481 gtgaattcga gctcggtacc cggggatcct ctagagtcga cctgcaggca tgcaagcttg 541 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac 601 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc 661 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 721 cgaagacaag ggatccgcaa tgtgttatta agttgtctaa gcgtcaattt gtttacacca 781 caatatatcc tgccaccagc cagccaacag ctccccgacc ggcagctcgg cacaaaatca 841 ccactcgata caggcagccc atcagtccgg gacggcgtca gcgggagagc cgttgtaagg 901 cggcagactt tgctcatgtt //