LOCUS       Exported                1006 bp ds-DNA     linear   SYN 14-JUN-2021
DEFINITION  empty vector (lacZ) for 2nd generation recipient assembly.
ACCESSION   .
VERSION     .
KEYWORDS    pJOG292
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, 
            Boch J, Stuttmann J
  TITLE     Generation of chromosomal deletions in dicotyledonous plants 
            employing a user-friendly genome editing toolkit.
  JOURNAL   Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319.
  PUBMED    27579989
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 14, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    714..738
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    843..864
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        879..909
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    917..933
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     922..944
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     941..957
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     941..957
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ctcgccgcgt tgtttcatca agccttacgg tcaccgtaac cagcaaatca atatcactgt
       61 gtggcttcag gccgccatcc actgcggagc cgtacaaatg tacggccagc aacgtcggtt
      121 cgagatggcg ctcgatgacg ccaactacct ctgatagttg agtcgatact tcggcgatca
      181 ccgcttcccc catgatgttt aactttgttt tagggcgact gccctgctgc gtaacatcgt
      241 tgctgctcca taacatcaaa catcgaccca cggcgtaacg cgcttgctgc ttggatgccc
      301 gaggcataga ctgtacccca aaaaaacagt cataacaagc catgaaaacc gccactgcgc
      361 cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt gcgtgagcgc atacgctact
      421 tgcattacag cttacgaacc gaacaggctt atgtccactg ggttcgtgcc cgaattgatc
      481 acaggcagca acgctctgtc atcgttacaa tcagcatgct accctccgcg agatcatccg
      541 tgtttaaaac ccggcagctt agttgccgtt cttccgaata gcatcggtaa catgagcaaa
      601 gtctgccgcc ttacaacggc tctcccgctg acgccgtccc ggactgatgg gctgcctgta
      661 tcgagtggtg attttgtgcc gagctgccgg tcggggagct gttggctggc tggtggcagg
      721 atatattgtg gtgtaaacaa attgacgctt agacaactta ataacacatt gcggatccct
      781 tgtcttcgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
      841 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
      901 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
      961 gattacgcca agcttgcatg cctgcaggtc gactctagag gatccc
//
