LOCUS       Exported                 880 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  Lentiviral shRNA vector (pGIPz based) for inducible knockdown.
ACCESSION   .
VERSION     .
KEYWORDS    pInducer10-mir-RUP-PheS
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Meerbrey KL, Hu G, Kessler JD, Roarty K, Li MZ, Fang JE, 
            Herschkowitz JI, Burrows AE, Ciccia A, Sun T, Schmitt EM, Bernardi 
            RJ, Fu X, Bland CS, Cooper TA, Schiff R, Rosen JM, Westbrook TF, 
            Elledge SJ
  TITLE     The pINDUCER lentiviral toolkit for inducible RNA interference in 
            vitro and in vivo.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Mar 1;108(9):3665-70. doi: 
            10.1073/pnas.1019736108. Epub 2011 Feb 9.
  PUBMED    21307310
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..880
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     ncRNA           89..182
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     182..198
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     183..199
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             220..237
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             238..261
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     regulatory      271..280
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
ORIGIN
        1 agtactgcga cctccctagc aaactggggc acagatgatg cggccgcaag ccttgttaag
       61 tgctcgcttc ggcagcacat atactatgtt gaatgaggct tcagtacttt acagaatcgt
      121 tgcctgcaca tcttggaaac acttgctggg attacttctt caggttaacc caacagaagg
      181 ctcgaggtcg acggtatcga taagcttgac gtccatatgc tggtaccccg tggaagtctg
      241 gaagttctct tccaggggcc cgaaggagac gccaccatgg gtctagatag ggataacagg
      301 gtaatggtac cggatcgatc ccccaacttc caggcccgcc atagtgattt gatttgccag
      361 cgcatcgcta tcaatcgccg ggttcaccca ttcgcgtaac cacagttcac tgaatttcat
      421 aatctattcc tgccttattt aaactgtttg aggaaacgca gatcgttttc gaagaatgaa
      481 cgcaggtcgg tgacgccgta acgcaacata gtcagacgct ccatccccat cccgaagccg
      541 aaaccagagt aaacttccgg gtcgatgcca acgttacgca acacgttcgg atgcaccatc
      601 ccgcagccca gcacttccag ccatttaccg tttttaccca tgacgtccac ttctgcagaa
      661 ggttcggtaa acgggaagta ggaaggacgg aagcgaatct gcaaatcttc ctcaaagaag
      721 ttacgcagga agtcgtgcag cgtgcctttc aggttggtaa agctgatgtt ggtatcaaca
      781 atcagacctt ccatctgatg gaacatcggc gtgtgagtct ggtcgtagtc gttacgataa
      841 acacggccag gcgcgatgat acgaatcggt ggctgctggg
//
