LOCUS Exported 10657 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pLOX-gfp-iresTK SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10657) AUTHORS Salmon P, Oberholzer J, Occhiodoro T, Morel P, Lou J, Trono D TITLE Reversible immortalization of human primary cells by lentivector-mediated transfer of specific genes. JOURNAL Mol Ther. 2000 Oct . 2(4):404-14. PUBMED 11020357 REFERENCE 2 (bases 1 to 10657) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10657 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 4..637 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 684..809 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1302..1535 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1720..1764 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" enhancer 2043..2346 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 2347..2550 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 2501..2521 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 2547..2571 /label=LNCX /note="Human CMV promoter, forward primer" regulatory 2651..2660 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 2657..3373 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 2657..3373 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(2702..2723) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(2963..2982) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 3310..3331 /label=EGFP-C /note="EGFP, forward primer" misc_feature 3469..3930 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(3525..3542) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 3752..3771 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 3935..5065 /codon_start=1 /product="herpes simplex virus thymidine kinase" /label=HSV TK /note="confers sensitivity to nucleoside analogs such as acyclovir or ganciclovir" /translation="MASYPCHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRLEQK MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQVLGASETIANIYTTQ HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR PGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAVPPQGAEPQSNAGPR PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDAL LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN" protein_bind 5221..5254 /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." LTR 5274..5454 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" promoter complement(5479..5497) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(5480..5497) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(5787..5806) /label=pBRrevBam /note="pBR322 vectors, tet region, downstream of BamHI, reverse primer" primer_bind complement(6020..6039) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 6139..6161 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(6199..6217) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 6285..6389 /gene="bla" /label=AmpR promoter CDS 6390..7250 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(6608..6627) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 7421..8009 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 7910..7929 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 8163..8180 /label=L4440 /note="L4440 vector, forward primer" primer_bind complement(8250..8270) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 8255..8584 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 8435..8570 /label=SV40 ori /note="SV40 origin of replication" primer_bind 8497..8516 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" intron 9718..9783 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" CDS 9913..9933 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" polyA_signal 10358..10492 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(10395..10414) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 10449..10468 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 agttggaagg gctaattcac tcccaaagaa gacaagatat ccttgatctg tggatctacc 61 acacacaagg ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc 121 cactgacctt tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg 181 ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc 241 cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc 301 gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt 361 ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca 421 gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc 481 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg 541 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc 601 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga 661 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca 721 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct 781 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat 841 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat 901 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag 961 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta 1021 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag 1081 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac 1141 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt 1201 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca 1261 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg 1321 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc 1381 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg 1441 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg 1501 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa 1561 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag 1621 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta 1681 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg 1741 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat 1801 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta 1861 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc 1921 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac 1981 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgataagc ttgggagttc 2041 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca 2101 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt 2161 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg 2221 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag 2281 tacatgacct tacgggactt tcctacttgg cagtacatct acgtattagt catcgctatt 2341 accatggtga tgcggttttg gcagtacacc aatgggcgtg gatagcggtt tgactcacgg 2401 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa 2461 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt 2521 gtacggtggg aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga 2581 cgccatccac gctgttttga cctccataga agacaccgac tctagctaga ggatcctagc 2641 gctaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat 2701 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga 2761 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc 2821 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta 2881 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca 2941 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt 3001 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg 3061 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc 3121 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg 3181 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct 3241 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa 3301 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga 3361 cgagctgtac aagtccggac tcagatctcg agctcaagct tcgaattaat tctgcagtcg 3421 acattaattc cggttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa 3481 acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat 3541 gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac 3601 aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg 3661 cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt 3721 tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg 3781 gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac 3841 atgctttaca tgtgtttagt cgaggttaaa aaacgtctag gccccccgaa ccacggggac 3901 gtggttttcc tttgaaaaac acgatgataa taccatggct tcgtacccct gccatcaaca 3961 cgcgtctgcg ttcgaccagg ctgcgcgttc tcgcggccat agcaaccgac gtacggcgtt 4021 gcgccctcgc cggcagcaag aagccacgga agtccgcctg gagcagaaaa tgcccacgct 4081 actgcgggtt tatatagacg gtcctcacgg gatggggaaa accaccacca cgcaactgct 4141 ggtggccctg ggttcgcgcg acgatatcgt ctacgtaccc gagccgatga cttactggca 4201 ggtgctgggg gcttccgaga caatcgcgaa catctacacc acacaacacc gcctcgacca 4261 gggtgagata tcggccgggg acgcggcggt ggtaatgaca agcgcccaga taacaatggg 4321 catgccttat gccgtgaccg acgccgttct ggctcctcat atcggggggg aggctgggag 4381 ctcacatgcc ccgcccccgg ccctcaccct catcttcgac cgccatccca tcgccgccct 4441 cctgtgctac ccggccgcgc gataccttat gggcagcatg accccccagg ccgtgctggc 4501 gttcgtggcc ctcatcccgc cgaccttgcc cggcacaaac atcgtgttgg gggcccttcc 4561 ggaggacaga cacatcgacc gcctggccaa acgccagcgc cccggcgagc ggcttgacct 4621 ggctatgctg gccgcgattc gccgcgttta cgggctgctt gccaatacgg tgcggtatct 4681 gcagggcggc gggtcgtggc gggaggattg gggacagctt tcggggacgg ccgtgccgcc 4741 ccagggtgcc gagccccaga gcaacgcggg cccacgaccc catatcgggg acacgttatt 4801 taccctgttt cgggcccccg agttgctggc ccccaacggc gacctgtaca acgtgtttgc 4861 ctgggccttg gacgtcttgg ccaaacgcct ccgtcccatg cacgtcttta tcctggatta 4921 cgaccaatcg cccgccggct gccgggacgc cctgctgcaa cttacctccg ggatggtcca 4981 gacccacgtc accacccccg gctccatacc gacgatctgc gacctggcgc gcacgtttgc 5041 ccgggagatg ggggaggcta actgaaacac ggaaggggta ccgaattcct cgacggtacc 5101 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg 5161 gggactggaa gggctaattc actcccaacg aagacaagat gggatcaatt caccatggga 5221 ataacttcgt atagcataca ttatacgaag ttatgctgct ttttgcttgt actgggtctc 5281 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 5341 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 5401 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagcatct 5461 agaattaatt ccgtgtattc tatagtgtca cctaaatcgt atgtgtatga tacataaggt 5521 tatgtattaa ttgtagccgc gttctaacga caatatgtac aagcctaatt gtgtagcatc 5581 tggcttactg aagcagaccc tatcatctct ctcgtaaact gccgtcagag tcggtttggt 5641 tggacgaacc ttctgagttt ctggtaacgc cgtcccgcac ccggaaatgg tcagcgaacc 5701 aatcagcagg gtcatcgcta gccagatcct ctacgccgga cgcatcgtgg ccggcatcac 5761 cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg 5821 ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt 5881 ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct 5941 caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg 6001 tcgaatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac 6061 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca 6121 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga 6181 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa 6241 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt 6301 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa 6361 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta 6421 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag 6481 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca 6541 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta 6601 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc 6661 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc 6721 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca 6781 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc 6841 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca 6901 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac 6961 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg 7021 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg 7081 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg 7141 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac 7201 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc 7261 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct 7321 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc 7381 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc 7441 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg 7501 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa 7561 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc 7621 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt 7681 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa 7741 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc 7801 tacagcgtga gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc 7861 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct 7921 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat 7981 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc 8041 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg 8101 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc 8161 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg 8221 cgcgttggcc gattcattaa tgcagctgtg gaatgtgtgt cagttagggt gtggaaagtc 8281 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag 8341 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta 8401 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc 8461 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc 8521 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg 8581 caaaaagctt ggacacaaga caggcttgcg agatatgttt gagaatacca ctttatcccg 8641 cgtcagggag aggcagtgcg taaaaagacg cggactcatg tgaaatactg gtttttagtg 8701 cgccagatct ctataatctc gcgcaaccta ttttcccctc gaacactttt taagccgtag 8761 ataaacaggc tgggacactt cacatgagcg aaaaatacat cgtcacctgg gacatgttgc 8821 agatccatgc acgtaaactc gcaagccgac tgatgccttc tgaacaatgg aaaggcatta 8881 ttgccgtaag ccgtggcggt ctgtaccggg tgcgttactg gcgcgtgaac tgggtattcg 8941 tcatgtcgat accgtttgta tttccagcta cgatcacgac aaccagcgcg agcttaaagt 9001 gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt attgatgacc tggtggatac 9061 cggtggtact gcggttgcga ttcgtgaaat gtatccaaaa gcgcactttg tcaccatctt 9121 cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt gttgatatcc cgcaagatac 9181 ctggattgaa cagccgtggg atatgggcgt cgtattcgtc ccgccaatct ccggtcgcta 9241 atcttttcaa cgcctggcac tgccgggcgt tgttcttttt aacttcaggc gggttacaat 9301 agtttccagt aagtattctg gaggctgcat ccatgacaca ggcaaacctg agcgaaaccc 9361 tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct agtccgccgc tttaatcacg 9421 gcgcacaacc gcctgtgcag tcggcccttg atggtaaaac catccctcac tggtatcgca 9481 tgattaaccg tctgatgtgg atctggcgcg gcattgaccc acgcgaaatc ctcgacgtcc 9541 aggcacgtat tgtgatgagc gatgccgaac gtaccgacga tgatttatac gatacggtga 9601 ttggctaccg tggcggcaac tggatttatg agtgggcccc ggatctttgt gaaggaacct 9661 tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag ctctaaggta 9721 aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt ttgtgtattt 9781 tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa 9841 aacctgtttt gctcagaaga aatgccatct agtgatgatg aggctactgc tgactctcaa 9901 cattctactc ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt tccttcagaa 9961 ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt 10021 tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa atattctgta 10081 acctttataa gtaggcataa cagttataat cataacatac tgttttttct tactccacac 10141 aggcatagag tgtctgctat taataactat gctcaaaaat tgtgtacctt tagcttttta 10201 atttgtaaag gggttaataa ggaatatttg atgtatagtg ccttgactag agatcataat 10261 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct 10321 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa 10381 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 10441 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat 10501 aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa 10561 ttacctagtg gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa 10621 gaaattgtat ttgttaaata tgtactacaa acttagt //