LOCUS Exported 798 bp ds-DNA linear SYN 25-MAY-2021 DEFINITION For generating adeno-associated virus to express RandE3 under the EF1a promoter in a 'Cre-on' dependent manner.. ACCESSION . VERSION . KEYWORDS pAAV-Ef1a-DIO_EGFP-RandE3 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 798) AUTHORS Gross GG, Straub C, Perez-Sanchez J, Dempsey WP, Junge JA, Roberts RW, Trinh LA, Fraser SE, De Koninck Y, De Koninck P, Sabatini BL, Arnold DB TITLE An E3-ligase-based method for ablating inhibitory synapses. JOURNAL Nat Methods. 2016 Jun 6. doi: 10.1038/nmeth.3894. PUBMED 27271196 REFERENCE 2 (bases 1 to 798) AUTHORS . TITLE Direct Submission JOURNAL Exported May 25, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..798 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 48..81 /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." protein_bind 132..165 /label=lox2272 /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GGATACTT). lox2272 sites are compatible with each other, but incompatible with loxP or loxN sites (Lee and Saito, 1988)." CDS 175..696 /codon_start=1 /product="N-terminal fragment of mVenus for use in bimolecular fluorescence complementation (BiFC) (Kodama and Hu, 2010)" /label=VN173 /translation="MGVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKD DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGI KVNFKIRHNIE" primer_bind complement(223..244) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(484..503) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" ORIGIN 1 attttgtaat ccagaggttg attatcgata agcttgatat cgaattcata acttcgtata 61 gcatacatta tacgaagtta ttctttgcac cattctaaag aataacagtg ataatttctg 121 ggttaaggca aataacttcg tataggatac tttatacgaa gttatgctag gcatatgggg 181 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc 241 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc 301 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc 361 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag 421 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc 481 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg 541 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag 601 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc 661 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac 721 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac 781 ctgagcaccc agtccgcc //